Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells
Background/purpose: Naringenin, a naturally occurring flavanone in citrus fruits, regulates bone formation by bone marrow-derived mesenchymal stem cells. The purpose of this study was to characterize the effects of naringenin on some biological behaviors of human dental pulp stem cells (HDPSCs). Mat...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790222002124 |
| _version_ | 1797862166429171712 |
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| author | Yeon Kim Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Jacques E. Nör Moon-Kyoung Bae |
| author_facet | Yeon Kim Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Jacques E. Nör Moon-Kyoung Bae |
| author_sort | Yeon Kim |
| collection | DOAJ |
| description | Background/purpose: Naringenin, a naturally occurring flavanone in citrus fruits, regulates bone formation by bone marrow-derived mesenchymal stem cells. The purpose of this study was to characterize the effects of naringenin on some biological behaviors of human dental pulp stem cells (HDPSCs). Materials and methods: HDPSCs were cultured in osteogenic differentiation medium and osteo/odontogenic differentiation and mineralization were analyzed by alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining. The migration of HDPSCs was evaluated by transwell chemotactic migration assays and scratch wound healing migration assay. Using tooth slice/scaffold model, we assessed the in vivo odontogenic differentiation potential of HDPSCs. Results: We have demonstrated that naringenin increases the osteogenic/odontogenic differentiation of HDPSCs through regulation of osteogenic-related proteins and the migratory ability of HDPSCs through stromal cell derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4) axis. Moreover, naringenin promotes the expression of dentin matrix acidic phosphoprotein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) in HDPSCs seeded on tooth slice/scaffolds that are subcutaneously implanted into immunodeficient mice. Conclusion: Our present study suggests that naringenin promotes migration and osteogenic/odontogenic differentiation of HDPSCs and may serve as a promising candidate in dental tissue engineering and bone regeneration. |
| first_indexed | 2024-04-09T22:14:50Z |
| format | Article |
| id | doaj.art-3458e25b87a2422c93ad98cd22870fb4 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-09T22:14:50Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-3458e25b87a2422c93ad98cd22870fb42023-03-23T04:34:59ZengElsevierJournal of Dental Sciences1991-79022023-04-01182577585Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cellsYeon Kim0Hyun-Joo Park1Mi-Kyoung Kim2Yong-Il Kim3Hyung Joon Kim4Soo-Kyung Bae5Jacques E. Nör6Moon-Kyoung Bae7Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South KoreaDepartment of Orthodontics, School of Dentistry, Pusan National University, Yangsan, South KoreaDepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South KoreaPeriodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea; Department of Dental Pharmacology, School of Dentistry, Pusan National University, Yangsan, South KoreaDepartment of Cariology, Restorative Sciences, Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI, USADepartment of Oral Physiology, School of Dentistry, Pusan National University, Yangsan, South Korea; Periodontal Disease Signaling Network Research Center (MRC), Pusan National University, Yangsan, South Korea; Dental and Life Science Institute, Pusan National University, Yangsan, South Korea; Corresponding author. Department of Oral Physiology, School of Dentistry, Pusan National University, 49 Busandaehak-ro, Yangsan 50612, South Korea.Background/purpose: Naringenin, a naturally occurring flavanone in citrus fruits, regulates bone formation by bone marrow-derived mesenchymal stem cells. The purpose of this study was to characterize the effects of naringenin on some biological behaviors of human dental pulp stem cells (HDPSCs). Materials and methods: HDPSCs were cultured in osteogenic differentiation medium and osteo/odontogenic differentiation and mineralization were analyzed by alkaline phosphatase (ALP) staining and Alizarin Red S (ARS) staining. The migration of HDPSCs was evaluated by transwell chemotactic migration assays and scratch wound healing migration assay. Using tooth slice/scaffold model, we assessed the in vivo odontogenic differentiation potential of HDPSCs. Results: We have demonstrated that naringenin increases the osteogenic/odontogenic differentiation of HDPSCs through regulation of osteogenic-related proteins and the migratory ability of HDPSCs through stromal cell derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4) axis. Moreover, naringenin promotes the expression of dentin matrix acidic phosphoprotein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) in HDPSCs seeded on tooth slice/scaffolds that are subcutaneously implanted into immunodeficient mice. Conclusion: Our present study suggests that naringenin promotes migration and osteogenic/odontogenic differentiation of HDPSCs and may serve as a promising candidate in dental tissue engineering and bone regeneration.http://www.sciencedirect.com/science/article/pii/S1991790222002124NaringeninHuman dental pulp stem cellsDifferentiationMigration |
| spellingShingle | Yeon Kim Hyun-Joo Park Mi-Kyoung Kim Yong-Il Kim Hyung Joon Kim Soo-Kyung Bae Jacques E. Nör Moon-Kyoung Bae Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells Journal of Dental Sciences Naringenin Human dental pulp stem cells Differentiation Migration |
| title | Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells |
| title_full | Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells |
| title_fullStr | Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells |
| title_full_unstemmed | Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells |
| title_short | Naringenin stimulates osteogenic/odontogenic differentiation and migration of human dental pulp stem cells |
| title_sort | naringenin stimulates osteogenic odontogenic differentiation and migration of human dental pulp stem cells |
| topic | Naringenin Human dental pulp stem cells Differentiation Migration |
| url | http://www.sciencedirect.com/science/article/pii/S1991790222002124 |
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