Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology
The early detection of infectious diseases and microorganisms is critical for effective disease treatment, control, and prevention. Currently, nucleic acid testing and antigen–antibody serum reaction are the two methods most commonly used for the detection of infectious diseases. The former is highl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/13/13/2255 |
| _version_ | 1797591888380821504 |
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| author | Shiu-Jau Chen Chung-I Rai Shao-Cheng Wang Yuan-Chuan Chen |
| author_facet | Shiu-Jau Chen Chung-I Rai Shao-Cheng Wang Yuan-Chuan Chen |
| author_sort | Shiu-Jau Chen |
| collection | DOAJ |
| description | The early detection of infectious diseases and microorganisms is critical for effective disease treatment, control, and prevention. Currently, nucleic acid testing and antigen–antibody serum reaction are the two methods most commonly used for the detection of infectious diseases. The former is highly accurate, specific, and sensitive, but it is time-consuming, expensive, and has special technician and instrument requirements. The latter is rapid and economical, but it may not be accurate and sensitive enough. Therefore, it is necessary to develop a quick and on-site diagnostic test for point-of-care testing (POCT) to enable the clinical detection of infectious diseases that is accurate, sensitive, convenient, cheap, and portable. Here, CRISPR/Cas-based detection methods are detailed and discussed in depth. The powerful capacity of these methods will facilitate the development of diagnostic tools for POCT, though they still have some limitations. This review explores and highlights POCT based on the class 2 CRISPR/Cas assay, such as Cas12 and Cas13 proteins, for the detection of infectious diseases. We also provide an outlook on perspectives, multi-application scenarios, clinical applications, and limitations for POCT based on class 2 CRISPR/Cas technology. |
| first_indexed | 2024-03-11T01:43:48Z |
| format | Article |
| id | doaj.art-34592b7c027244c7a03242ba1c027c73 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T01:43:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-34592b7c027244c7a03242ba1c027c732023-11-18T16:22:08ZengMDPI AGDiagnostics2075-44182023-07-011313225510.3390/diagnostics13132255Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas TechnologyShiu-Jau Chen0Chung-I Rai1Shao-Cheng Wang2Yuan-Chuan Chen3Department of Neurosurgery, Mackay Memorial Hospital, Taipei 10449, TaiwanHealth Care Business Group, Foxconn Technology Co., Ltd., New Taipei City 23680, TaiwanDepartment of Psychiatric, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan 33004, TaiwanDepartment of Nursing, Jenteh Junior College of Medicine, Nursing and Management, Miaoli County 35664, TaiwanThe early detection of infectious diseases and microorganisms is critical for effective disease treatment, control, and prevention. Currently, nucleic acid testing and antigen–antibody serum reaction are the two methods most commonly used for the detection of infectious diseases. The former is highly accurate, specific, and sensitive, but it is time-consuming, expensive, and has special technician and instrument requirements. The latter is rapid and economical, but it may not be accurate and sensitive enough. Therefore, it is necessary to develop a quick and on-site diagnostic test for point-of-care testing (POCT) to enable the clinical detection of infectious diseases that is accurate, sensitive, convenient, cheap, and portable. Here, CRISPR/Cas-based detection methods are detailed and discussed in depth. The powerful capacity of these methods will facilitate the development of diagnostic tools for POCT, though they still have some limitations. This review explores and highlights POCT based on the class 2 CRISPR/Cas assay, such as Cas12 and Cas13 proteins, for the detection of infectious diseases. We also provide an outlook on perspectives, multi-application scenarios, clinical applications, and limitations for POCT based on class 2 CRISPR/Cas technology.https://www.mdpi.com/2075-4418/13/13/2255CRISPRCas9Cas12aCas13DETECTRSHERLOCK |
| spellingShingle | Shiu-Jau Chen Chung-I Rai Shao-Cheng Wang Yuan-Chuan Chen Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology Diagnostics CRISPR Cas9 Cas12a Cas13 DETECTR SHERLOCK |
| title | Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology |
| title_full | Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology |
| title_fullStr | Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology |
| title_full_unstemmed | Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology |
| title_short | Point-of-Care Testing for Infectious Diseases Based on Class 2 CRISPR/Cas Technology |
| title_sort | point of care testing for infectious diseases based on class 2 crispr cas technology |
| topic | CRISPR Cas9 Cas12a Cas13 DETECTR SHERLOCK |
| url | https://www.mdpi.com/2075-4418/13/13/2255 |
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