Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate
Abstract Whole‐room indirect calorimeters (WRICs) provide accurate instruments for the measurement of respiratory exchange, energy expenditure, and macronutrient oxidation. Here, we aimed to determine the validity and reproducibility of a 7500 L WRIC for the measurement of ventilation rates and rest...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-04-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15658 |
| _version_ | 1797397125480316928 |
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| author | Hege Berg Henriksen Christine Henriksen Ana Rita Sequeira deSousa Dena Treider Alavi Elin Maria Sandstad Augestad Russell Rising Rebecca Dörner Franziska Anna Hägele Anja Bosy‐Westphal Rune Blomhoff Stine Marie Ulven Thomas Olsen |
| author_facet | Hege Berg Henriksen Christine Henriksen Ana Rita Sequeira deSousa Dena Treider Alavi Elin Maria Sandstad Augestad Russell Rising Rebecca Dörner Franziska Anna Hägele Anja Bosy‐Westphal Rune Blomhoff Stine Marie Ulven Thomas Olsen |
| author_sort | Hege Berg Henriksen |
| collection | DOAJ |
| description | Abstract Whole‐room indirect calorimeters (WRICs) provide accurate instruments for the measurement of respiratory exchange, energy expenditure, and macronutrient oxidation. Here, we aimed to determine the validity and reproducibility of a 7500 L WRIC for the measurement of ventilation rates and resting metabolic rate (RMR). Technical validation was performed with propane combustion tests (n = 10) whereas biological reproducibility was tested in healthy subjects (13 women, 6 men, mean ± SD age 39.6 ± 15.3) in two 60 min measurements separated by 24 h. Subjects followed a run‐in protocol prior to measurements. The coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated for ventilation rates of O2 (VO2), CO2 (VCO2), the respiratory quotient (RQ; VCO2/VO2), and RMR. Technical validation showed good validity with CVs ranging from 0.67% for VO2 to 1.00% for energy expenditure. For biological reproducibility, CVs were 2.89% for VO2; 2.67% for VCO2; 1.95% for RQ; and 2.68% for RMR. With the exception of RQ (74%), ICCs were excellent for VO2 (94%), VCO2 (96%) and RMR (95%). Excluding participants that deviated from the run‐in protocol did not alter results. In conclusion, the 7500 L WRIC is technically valid and reproducible for ventilation rates and RMR. |
| first_indexed | 2024-03-09T01:05:31Z |
| format | Article |
| id | doaj.art-345a3a24bdff48e59b8748342fd8b382 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:05:31Z |
| publishDate | 2023-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-345a3a24bdff48e59b8748342fd8b3822023-12-11T09:20:39ZengWileyPhysiological Reports2051-817X2023-04-01117n/an/a10.14814/phy2.15658Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rateHege Berg Henriksen0Christine Henriksen1Ana Rita Sequeira deSousa2Dena Treider Alavi3Elin Maria Sandstad Augestad4Russell Rising5Rebecca Dörner6Franziska Anna Hägele7Anja Bosy‐Westphal8Rune Blomhoff9Stine Marie Ulven10Thomas Olsen11Department of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayD & S Consulting Services, Inc. New York New York USAInstitute of Human Nutrition and Food Science Christian‐Albrechts University Kiel GermanyInstitute of Human Nutrition and Food Science Christian‐Albrechts University Kiel GermanyInstitute of Human Nutrition and Food Science Christian‐Albrechts University Kiel GermanyDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayDepartment of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences University of Oslo Oslo NorwayAbstract Whole‐room indirect calorimeters (WRICs) provide accurate instruments for the measurement of respiratory exchange, energy expenditure, and macronutrient oxidation. Here, we aimed to determine the validity and reproducibility of a 7500 L WRIC for the measurement of ventilation rates and resting metabolic rate (RMR). Technical validation was performed with propane combustion tests (n = 10) whereas biological reproducibility was tested in healthy subjects (13 women, 6 men, mean ± SD age 39.6 ± 15.3) in two 60 min measurements separated by 24 h. Subjects followed a run‐in protocol prior to measurements. The coefficient of variation (CV) and intraclass correlation coefficient (ICC) were calculated for ventilation rates of O2 (VO2), CO2 (VCO2), the respiratory quotient (RQ; VCO2/VO2), and RMR. Technical validation showed good validity with CVs ranging from 0.67% for VO2 to 1.00% for energy expenditure. For biological reproducibility, CVs were 2.89% for VO2; 2.67% for VCO2; 1.95% for RQ; and 2.68% for RMR. With the exception of RQ (74%), ICCs were excellent for VO2 (94%), VCO2 (96%) and RMR (95%). Excluding participants that deviated from the run‐in protocol did not alter results. In conclusion, the 7500 L WRIC is technically valid and reproducible for ventilation rates and RMR.https://doi.org/10.14814/phy2.15658energy expendituremetabolismresting metabolic ratewhole‐room indirect calorimetry |
| spellingShingle | Hege Berg Henriksen Christine Henriksen Ana Rita Sequeira deSousa Dena Treider Alavi Elin Maria Sandstad Augestad Russell Rising Rebecca Dörner Franziska Anna Hägele Anja Bosy‐Westphal Rune Blomhoff Stine Marie Ulven Thomas Olsen Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate Physiological Reports energy expenditure metabolism resting metabolic rate whole‐room indirect calorimetry |
| title | Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate |
| title_full | Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate |
| title_fullStr | Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate |
| title_full_unstemmed | Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate |
| title_short | Validity and reproducibility of a whole‐room indirect calorimeter for the estimation of VO2, VCO2, and resting metabolic rate |
| title_sort | validity and reproducibility of a whole room indirect calorimeter for the estimation of vo2 vco2 and resting metabolic rate |
| topic | energy expenditure metabolism resting metabolic rate whole‐room indirect calorimetry |
| url | https://doi.org/10.14814/phy2.15658 |
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