Identification of key genes involved in nasopharyngeal carcinoma
Abstract Introduction: Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. Objective: To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. Methods: GSE12452 was downloaded from the Gene Expression Omnibus database and included...
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Otorhinolaryngology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942017000600670&lng=en&tlng=en |
| _version_ | 1818956446489903104 |
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| author | Xue Jiang Lichun Feng Baoqiang Dai Liping Li Weiwei Lu |
| author_facet | Xue Jiang Lichun Feng Baoqiang Dai Liping Li Weiwei Lu |
| author_sort | Xue Jiang |
| collection | DOAJ |
| description | Abstract Introduction: Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. Objective: To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. Methods: GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software. Results: A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2→PTGS2 and OVOL1→CXCL10). Conclusion: PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma. |
| first_indexed | 2024-12-20T10:54:05Z |
| format | Article |
| id | doaj.art-345f39fcc3364377a4a87ef6e7716b7a |
| institution | Directory Open Access Journal |
| issn | 1808-8686 |
| language | English |
| last_indexed | 2024-12-20T10:54:05Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj.art-345f39fcc3364377a4a87ef6e7716b7a2022-12-21T19:43:10ZengElsevierBrazilian Journal of Otorhinolaryngology1808-868683667067610.1016/j.bjorl.2016.09.003S1808-86942017000600670Identification of key genes involved in nasopharyngeal carcinomaXue JiangLichun FengBaoqiang DaiLiping LiWeiwei LuAbstract Introduction: Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. Objective: To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. Methods: GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software. Results: A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2→PTGS2 and OVOL1→CXCL10). Conclusion: PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942017000600670&lng=en&tlng=enNasopharyngeal carcinomaDifferentially expressed genesEnrichment analysisRegulatory network |
| spellingShingle | Xue Jiang Lichun Feng Baoqiang Dai Liping Li Weiwei Lu Identification of key genes involved in nasopharyngeal carcinoma Brazilian Journal of Otorhinolaryngology Nasopharyngeal carcinoma Differentially expressed genes Enrichment analysis Regulatory network |
| title | Identification of key genes involved in nasopharyngeal carcinoma |
| title_full | Identification of key genes involved in nasopharyngeal carcinoma |
| title_fullStr | Identification of key genes involved in nasopharyngeal carcinoma |
| title_full_unstemmed | Identification of key genes involved in nasopharyngeal carcinoma |
| title_short | Identification of key genes involved in nasopharyngeal carcinoma |
| title_sort | identification of key genes involved in nasopharyngeal carcinoma |
| topic | Nasopharyngeal carcinoma Differentially expressed genes Enrichment analysis Regulatory network |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942017000600670&lng=en&tlng=en |
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