Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence
Abstract Paclitaxel (PTX) is one of the most effective drugs used in the treatment of breast cancer. Nonetheless, the appearance of MDR1 (multidrug resistance 1) in tumor cells has become a significant hindrance for efficacious chemotherapy. In this study, we show that the expression level of Egr-1...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-08-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100397&lng=en&tlng=en |
| _version_ | 1797733680560472064 |
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| author | Pei-yao Gong |
| author_facet | Pei-yao Gong |
| author_sort | Pei-yao Gong |
| collection | DOAJ |
| description | Abstract Paclitaxel (PTX) is one of the most effective drugs used in the treatment of breast cancer. Nonetheless, the appearance of MDR1 (multidrug resistance 1) in tumor cells has become a significant hindrance for efficacious chemotherapy. In this study, we show that the expression level of Egr-1 (early growth response gene-1) in cancer tissues (from paclitaxel chemotherapy failure patients) and MCF-7/PTX cells (the breast cancer cell line that was resistant to paclitaxel) was increased. Cell proliferation assay and apoptosis assay revealed that Egr-1 could promote cell growth and inhibit apoptosis in MCF-7/PTX. Mechanistic studies indicated that Egr-1 could bind to the proximal MDR1 promoter and enhance MDR1 transcription. These findings indicate that paclitaxel induced Egr-1 accumulation and upregulated the expression of MDR1, thereby inducing the drug resistance in MCF-7/PTX. Our results suggest a novel pathway by which paclitaxel induces MDR1 expression, possibly illuminating a potential target pathway for the prevention of MDR1-mediated drug resistance. |
| first_indexed | 2024-03-12T12:32:41Z |
| format | Article |
| id | doaj.art-3470133069504fd1afbc96324912a571 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-03-12T12:32:41Z |
| publishDate | 2023-08-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-3470133069504fd1afbc96324912a5712023-08-29T07:45:03ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-08-015910.1590/s2175-97902023e18705Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 DependencePei-yao Gonghttps://orcid.org/0000-0002-1355-5879Abstract Paclitaxel (PTX) is one of the most effective drugs used in the treatment of breast cancer. Nonetheless, the appearance of MDR1 (multidrug resistance 1) in tumor cells has become a significant hindrance for efficacious chemotherapy. In this study, we show that the expression level of Egr-1 (early growth response gene-1) in cancer tissues (from paclitaxel chemotherapy failure patients) and MCF-7/PTX cells (the breast cancer cell line that was resistant to paclitaxel) was increased. Cell proliferation assay and apoptosis assay revealed that Egr-1 could promote cell growth and inhibit apoptosis in MCF-7/PTX. Mechanistic studies indicated that Egr-1 could bind to the proximal MDR1 promoter and enhance MDR1 transcription. These findings indicate that paclitaxel induced Egr-1 accumulation and upregulated the expression of MDR1, thereby inducing the drug resistance in MCF-7/PTX. Our results suggest a novel pathway by which paclitaxel induces MDR1 expression, possibly illuminating a potential target pathway for the prevention of MDR1-mediated drug resistance.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100397&lng=en&tlng=enEgr-1Breast cancerMDR1Drug resistance. |
| spellingShingle | Pei-yao Gong Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence Brazilian Journal of Pharmaceutical Sciences Egr-1 Breast cancer MDR1 Drug resistance. |
| title | Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence |
| title_full | Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence |
| title_fullStr | Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence |
| title_full_unstemmed | Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence |
| title_short | Egr-1 Enhances Drug Resistance of Breast Cancer Cells by MDR1 Dependence |
| title_sort | egr 1 enhances drug resistance of breast cancer cells by mdr1 dependence |
| topic | Egr-1 Breast cancer MDR1 Drug resistance. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502023000100397&lng=en&tlng=en |
| work_keys_str_mv | AT peiyaogong egr1enhancesdrugresistanceofbreastcancercellsbymdr1dependence |