Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates
Nanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were e...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/13/24/3099 |
| _version_ | 1797379837290086400 |
|---|---|
| author | Georgia Geka Anastasia Kanioura Ioannis Kochylas Vlassis Likodimos Spiros Gardelis Anastasios Dimitriou Nikolaos Papanikolaou Kalliopi Chatzantonaki Ekaterina Charvalos Anastasios Economou Sotirios Kakabakos Panagiota Petrou |
| author_facet | Georgia Geka Anastasia Kanioura Ioannis Kochylas Vlassis Likodimos Spiros Gardelis Anastasios Dimitriou Nikolaos Papanikolaou Kalliopi Chatzantonaki Ekaterina Charvalos Anastasios Economou Sotirios Kakabakos Panagiota Petrou |
| author_sort | Georgia Geka |
| collection | DOAJ |
| description | Nanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were evaluated as substrates for the immunochemical detection of two ovarian cancer indicators, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). The substrates were prepared by metal-enhanced chemical etching of silicon wafers to create, in one step, silicon nanowires and silver nanoparticles on top of them. For both analytes, non-competitive immunoassays were developed using pairs of highly specific monoclonal antibodies, one for analyte capture on the substrate and the other for detection. In order to facilitate the identification of the immunocomplexes through a reaction with streptavidin labeled with Rhodamine Red-X, the detection antibodies were biotinylated. An in-house-developed optical set-up was used for photoluminescence signal measurements after assay completion. The detection limits achieved were 2.5 U/mL and 3.12 pM for CA125 and HE4, respectively, with linear dynamic ranges extending up to 500 U/mL for CA125 and up to 500 pM for HE4, covering the concentration ranges of both healthy and ovarian cancer patients. Thus, the proposed method could be implemented for the early diagnosis and/or prognosis and monitoring of ovarian cancer. |
| first_indexed | 2024-03-08T20:28:48Z |
| format | Article |
| id | doaj.art-34704097cfc5489fa559849f27845d52 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-08T20:28:48Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-34704097cfc5489fa559849f27845d522023-12-22T14:29:02ZengMDPI AGNanomaterials2079-49912023-12-011324309910.3390/nano13243099Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver SubstratesGeorgia Geka0Anastasia Kanioura1Ioannis Kochylas2Vlassis Likodimos3Spiros Gardelis4Anastasios Dimitriou5Nikolaos Papanikolaou6Kalliopi Chatzantonaki7Ekaterina Charvalos8Anastasios Economou9Sotirios Kakabakos10Panagiota Petrou11Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceImmunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceSection of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, GreeceSection of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, GreeceSection of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, GreeceInstitute of Nanoscience & Nanotechnology, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceInstitute of Nanoscience & Nanotechnology, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceMolecular Diagnosis Department, INVITROLABS S.A., 12251 Peristeri, GreeceMolecular Diagnosis Department, INVITROLABS S.A., 12251 Peristeri, GreeceDepartment of Chemistry, National and Kapodistrian, University of Athens, University Campus, 15771 Athens, GreeceImmunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceImmunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR “Demokritos”, 15341 Aghia Paraskevi, GreeceNanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were evaluated as substrates for the immunochemical detection of two ovarian cancer indicators, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). The substrates were prepared by metal-enhanced chemical etching of silicon wafers to create, in one step, silicon nanowires and silver nanoparticles on top of them. For both analytes, non-competitive immunoassays were developed using pairs of highly specific monoclonal antibodies, one for analyte capture on the substrate and the other for detection. In order to facilitate the identification of the immunocomplexes through a reaction with streptavidin labeled with Rhodamine Red-X, the detection antibodies were biotinylated. An in-house-developed optical set-up was used for photoluminescence signal measurements after assay completion. The detection limits achieved were 2.5 U/mL and 3.12 pM for CA125 and HE4, respectively, with linear dynamic ranges extending up to 500 U/mL for CA125 and up to 500 pM for HE4, covering the concentration ranges of both healthy and ovarian cancer patients. Thus, the proposed method could be implemented for the early diagnosis and/or prognosis and monitoring of ovarian cancer.https://www.mdpi.com/2079-4991/13/24/3099optical biosensorovarian cancerCA125HE4immunochemical detectionphotoluminescence |
| spellingShingle | Georgia Geka Anastasia Kanioura Ioannis Kochylas Vlassis Likodimos Spiros Gardelis Anastasios Dimitriou Nikolaos Papanikolaou Kalliopi Chatzantonaki Ekaterina Charvalos Anastasios Economou Sotirios Kakabakos Panagiota Petrou Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates Nanomaterials optical biosensor ovarian cancer CA125 HE4 immunochemical detection photoluminescence |
| title | Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates |
| title_full | Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates |
| title_fullStr | Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates |
| title_full_unstemmed | Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates |
| title_short | Cancer Marker Immunosensing through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates |
| title_sort | cancer marker immunosensing through surface enhanced photoluminescence on nanostructured silver substrates |
| topic | optical biosensor ovarian cancer CA125 HE4 immunochemical detection photoluminescence |
| url | https://www.mdpi.com/2079-4991/13/24/3099 |
| work_keys_str_mv | AT georgiageka cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT anastasiakanioura cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT ioanniskochylas cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT vlassislikodimos cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT spirosgardelis cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT anastasiosdimitriou cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT nikolaospapanikolaou cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT kalliopichatzantonaki cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT ekaterinacharvalos cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT anastasioseconomou cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT sotirioskakabakos cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates AT panagiotapetrou cancermarkerimmunosensingthroughsurfaceenhancedphotoluminescenceonnanostructuredsilversubstrates |