CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis
Objective Hepatocellular carcinoma (HCC) is a disease that is associated with high mortality; currently, there is no curative and reliable treatment. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) is the key signaling molecule in the wingless and INT-1/planar cell polarity (WNT/PCP) pathway....
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PeerJ Inc.
2019-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/7816.pdf |
| _version_ | 1797420399157313536 |
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| author | Xuefeng Gu Hongbo Li Ling Sha Yuan Mao Chuanbing Shi Wei Zhao |
| author_facet | Xuefeng Gu Hongbo Li Ling Sha Yuan Mao Chuanbing Shi Wei Zhao |
| author_sort | Xuefeng Gu |
| collection | DOAJ |
| description | Objective Hepatocellular carcinoma (HCC) is a disease that is associated with high mortality; currently, there is no curative and reliable treatment. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) is the key signaling molecule in the wingless and INT-1/planar cell polarity (WNT/PCP) pathway. This study aimed to elucidate the prognostic significance of CELSR3 in HCC patients. Methods The Cancer Genome Atlas (TCGA) database, the Cancer Cell Line Encyclopedia (CCLE) database and the Gene Expression Omnibus (GEO) database were used to analyze the expression of CELSR3 mRNA in HCC samples and cells. The relationship between CELSR3 mRNA and clinical features was assessed by the chi-square test. the diagnostic and predictive value of CELSR3 mRNA expression were analyzed using the receiver operating characteristic (ROC) curve. Kaplan–Meier curve and Cox regression analyses were performed to assess the prognostic value of CELSR3 mRNA in HCC patients. Finally, all three cohorts database was used for gene set enrichment analysis(GSEA) and the identification of CELSR3-related signal transduction pathways. Results The expression of CELSR3 mRNA was upregulated in HCC, and its expression was correlated with age (P = 0.025), tumor status (P = 0.022), clinical stage (P = 0.003), T classification (P = 0.010), vital status (P = 0.001), and relapse (P = 0.005). The ROC curve assessment indicated that CELSR3 mRNA expression has high diagnostic value in HCC and in the subgroup analysis of stage. In addition, the Kaplan-Meier curve and Cox analyses suggested that patients with high CELSR3 mRNA expression have a poor prognosis, indicating that CELSR3 mRNA is an independent prognostic factor for the overall survival of HCC patients. GSEA showed that GO somatic diversification of immune receptors, GO endonuclease activity, GO DNA repair complex and GO somatic cell DNA recombination, were differentially enriched in the meta-GEO cohort, the HCC cell line cohort and the TCGA cohort of the high CELSR3 mRNA expression phenotype. Conclusion Our results indicate that CELSR3 mRNA is involved in the progression of cancer and can be used as a biomarker for the prognosis of HCC patients. |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:01:58Z |
| publishDate | 2019-10-01 |
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| spelling | doaj.art-3470da24c199404c841cd218a40a0e4c2023-12-03T09:50:14ZengPeerJ Inc.PeerJ2167-83592019-10-017e781610.7717/peerj.7816CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosisXuefeng Gu0Hongbo Li1Ling Sha2Yuan Mao3Chuanbing Shi4Wei Zhao5Medical School, Southeast University, Nanjing, Jiangsu, ChinaDepartment of Hepatology, Infectious diseases Hospital Affliated to Soochow University, Suzhou, Jiangsu, ChinaDepartment of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu, ChinaDepartment of Hematology and Oncology, Geriatric Hospital of Nanjing Medical University, Jiangsu Province Geriatric Hospital, Nanjing, Jiangsu, ChinaDepartment of Pathology, Pukou District Central Hospital, Pukou Branch of Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, ChinaMedical School, Southeast University, Nanjing, Jiangsu, ChinaObjective Hepatocellular carcinoma (HCC) is a disease that is associated with high mortality; currently, there is no curative and reliable treatment. Cadherin EGF LAG seven-pass G-type receptor 3 (CELSR3) is the key signaling molecule in the wingless and INT-1/planar cell polarity (WNT/PCP) pathway. This study aimed to elucidate the prognostic significance of CELSR3 in HCC patients. Methods The Cancer Genome Atlas (TCGA) database, the Cancer Cell Line Encyclopedia (CCLE) database and the Gene Expression Omnibus (GEO) database were used to analyze the expression of CELSR3 mRNA in HCC samples and cells. The relationship between CELSR3 mRNA and clinical features was assessed by the chi-square test. the diagnostic and predictive value of CELSR3 mRNA expression were analyzed using the receiver operating characteristic (ROC) curve. Kaplan–Meier curve and Cox regression analyses were performed to assess the prognostic value of CELSR3 mRNA in HCC patients. Finally, all three cohorts database was used for gene set enrichment analysis(GSEA) and the identification of CELSR3-related signal transduction pathways. Results The expression of CELSR3 mRNA was upregulated in HCC, and its expression was correlated with age (P = 0.025), tumor status (P = 0.022), clinical stage (P = 0.003), T classification (P = 0.010), vital status (P = 0.001), and relapse (P = 0.005). The ROC curve assessment indicated that CELSR3 mRNA expression has high diagnostic value in HCC and in the subgroup analysis of stage. In addition, the Kaplan-Meier curve and Cox analyses suggested that patients with high CELSR3 mRNA expression have a poor prognosis, indicating that CELSR3 mRNA is an independent prognostic factor for the overall survival of HCC patients. GSEA showed that GO somatic diversification of immune receptors, GO endonuclease activity, GO DNA repair complex and GO somatic cell DNA recombination, were differentially enriched in the meta-GEO cohort, the HCC cell line cohort and the TCGA cohort of the high CELSR3 mRNA expression phenotype. Conclusion Our results indicate that CELSR3 mRNA is involved in the progression of cancer and can be used as a biomarker for the prognosis of HCC patients.https://peerj.com/articles/7816.pdfGEOThe Cancer Genome AtlasGSEAHCCCELSR3CCLE |
| spellingShingle | Xuefeng Gu Hongbo Li Ling Sha Yuan Mao Chuanbing Shi Wei Zhao CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis PeerJ GEO The Cancer Genome Atlas GSEA HCC CELSR3 CCLE |
| title | CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| title_full | CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| title_fullStr | CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| title_full_unstemmed | CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| title_short | CELSR3 mRNA expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| title_sort | celsr3 mrna expression is increased in hepatocellular carcinoma and indicates poor prognosis |
| topic | GEO The Cancer Genome Atlas GSEA HCC CELSR3 CCLE |
| url | https://peerj.com/articles/7816.pdf |
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