Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan
Abstract Background Sarcocephalus latifolius is used as a traditional medicine for curing many diseases in Sudan. The main objective of the current study was to determine the antioxidant activity and acetylcholinesterase inhibition (AChEI) of S. latifolius, and to estimate its total phenolic and fla...
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| Format: | Article |
| Language: | English |
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BMC
2017-05-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1772-6 |
| _version_ | 1819227063864786944 |
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| author | Alsiddig Osama Sufyan Awadelkarim Amna Ali |
| author_facet | Alsiddig Osama Sufyan Awadelkarim Amna Ali |
| author_sort | Alsiddig Osama |
| collection | DOAJ |
| description | Abstract Background Sarcocephalus latifolius is used as a traditional medicine for curing many diseases in Sudan. The main objective of the current study was to determine the antioxidant activity and acetylcholinesterase inhibition (AChEI) of S. latifolius, and to estimate its total phenolic and flavonoid contents. Methods Antioxidant activity of the tested plant extracts was carried out by determining their ability to scavenge the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical. On the other hand, AChE inhibitory activity was determined spectrophotometrically using the Ellman’s colorimetric method. The levels of total phenols and flavonoids were determined quantitatively using spectrophotometric methods. MTT assay was consumed to assess the cytotoxic effect of the most active fractions. These fractions were subjected to phytochemical analysis using GC-MS techniques to determine thier chemical composition. Results Hexane and chloroform fractions exhibited the highest antioxidant activity with IC50 values of (0.098 ± 0.08 and 0.099 ± 0.029 mg/ml) respectively. Standard propyl gallate had the lowest IC50 value of 0.0414 ± 0.11 mg/ml. The ethanolic crude extract showed low AChEI activity with 40.2 ± 0.10%. High concentrations of phenolic and flavonoid contents were observed. GCMS revealed the presence of well-known antioxidants compounds e.g. Vitamin E and caffeic acid. Conclusion The ethanolic extract of bark of S. latifolius showed potent antioxidant effects and low AChEI activity, high phenolic and flavonoid contents and presence of pharmacologically active compounds. These findings explain its wide usages in traditional medicine. |
| first_indexed | 2024-12-23T10:35:26Z |
| format | Article |
| id | doaj.art-347b0ceff4234d1b9e81fea04ec57b41 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-23T10:35:26Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-347b0ceff4234d1b9e81fea04ec57b412022-12-21T17:50:18ZengBMCBMC Complementary and Alternative Medicine1472-68822017-05-0117111010.1186/s12906-017-1772-6Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in SudanAlsiddig Osama0Sufyan Awadelkarim1Amna Ali2Chemistry Department, Omdurman Islamic UniversityChemistry Department, Omdurman Islamic UniversityMedical Biochemistry Research Unit, Medicinal and Aromatic Plants Research Institute, National Centre for ResearchAbstract Background Sarcocephalus latifolius is used as a traditional medicine for curing many diseases in Sudan. The main objective of the current study was to determine the antioxidant activity and acetylcholinesterase inhibition (AChEI) of S. latifolius, and to estimate its total phenolic and flavonoid contents. Methods Antioxidant activity of the tested plant extracts was carried out by determining their ability to scavenge the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical. On the other hand, AChE inhibitory activity was determined spectrophotometrically using the Ellman’s colorimetric method. The levels of total phenols and flavonoids were determined quantitatively using spectrophotometric methods. MTT assay was consumed to assess the cytotoxic effect of the most active fractions. These fractions were subjected to phytochemical analysis using GC-MS techniques to determine thier chemical composition. Results Hexane and chloroform fractions exhibited the highest antioxidant activity with IC50 values of (0.098 ± 0.08 and 0.099 ± 0.029 mg/ml) respectively. Standard propyl gallate had the lowest IC50 value of 0.0414 ± 0.11 mg/ml. The ethanolic crude extract showed low AChEI activity with 40.2 ± 0.10%. High concentrations of phenolic and flavonoid contents were observed. GCMS revealed the presence of well-known antioxidants compounds e.g. Vitamin E and caffeic acid. Conclusion The ethanolic extract of bark of S. latifolius showed potent antioxidant effects and low AChEI activity, high phenolic and flavonoid contents and presence of pharmacologically active compounds. These findings explain its wide usages in traditional medicine.http://link.springer.com/article/10.1186/s12906-017-1772-6S. latifoliusAntioxidantAcetylcholinesterasePhenolicsFlavonoidsGcms |
| spellingShingle | Alsiddig Osama Sufyan Awadelkarim Amna Ali Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan BMC Complementary and Alternative Medicine S. latifolius Antioxidant Acetylcholinesterase Phenolics Flavonoids Gcms |
| title | Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan |
| title_full | Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan |
| title_fullStr | Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan |
| title_full_unstemmed | Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan |
| title_short | Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. bark used in traditional medicine in Sudan |
| title_sort | antioxidant activity acetylcholinesterase inhibitory potential and phytochemical analysis of sarcocephalus latifolius sm bark used in traditional medicine in sudan |
| topic | S. latifolius Antioxidant Acetylcholinesterase Phenolics Flavonoids Gcms |
| url | http://link.springer.com/article/10.1186/s12906-017-1772-6 |
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