Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance
More than 180 mammalian species have been found naturally infected with <i>Trypanosoma cruzi</i>. Many of them play an important role in the maintenance of this parasite. In particular, new studies have appeared which indicate that some species of Procyonidae family may play a role as &l...
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MDPI AG
2020-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/6/464 |
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| author | Guiehdani Villalobos Claudia I. Muñoz-García Roberto Rodríguez-Cabo-Mercado Nancy Mendoza-Bazán Adrián Hernández-Ortiz Claudia Villanueva-García Fernando Martínez-Hernández Emilio Rendón-Franco |
| author_facet | Guiehdani Villalobos Claudia I. Muñoz-García Roberto Rodríguez-Cabo-Mercado Nancy Mendoza-Bazán Adrián Hernández-Ortiz Claudia Villanueva-García Fernando Martínez-Hernández Emilio Rendón-Franco |
| author_sort | Guiehdani Villalobos |
| collection | DOAJ |
| description | More than 180 mammalian species have been found naturally infected with <i>Trypanosoma cruzi</i>. Many of them play an important role in the maintenance of this parasite. In particular, new studies have appeared which indicate that some species of Procyonidae family may play a role as <i>T. cruzi</i> hosts, however, more data are needed to evaluate their long-term physiological response to parasite infection, especially for specific antibodies. In this study, antibodies to <i>T. cruzi</i> were detected and prevalence and epitope recognition were assessed by ELISA (using discrete typing unit (DTU) I as antigen) and WB (using DTU I and DTU II as antigens) and sera from two procyonid species obtained through five-year follow-up of two semicaptive populations living in the same habitat. Marked heterogeneity in antigens recognition between species and differences in seroprevalence (<i>p</i> = 0.0002) between white-nosed coatis (<i>Nasua narica</i>), 51.8% (115/222), and common raccoons (<i>Procyon lotor</i>), 28.3% (23/81), were found. Antigens with high molecular weight when DTU-I was used were the most recognized, while a greater antigen diversity recognition was observed with DTU-II; for white-nosed coatis, low-molecular-weight antigens were mainly recognized, while for common raccoons proteins with molecular weights greater than 80 kDa were recognized most. These divergent humoral immune responses could be related to an alleged pattern of recognition receptors and major histocompatibility complex molecules difference in the procyonids species. |
| first_indexed | 2024-03-10T19:12:48Z |
| format | Article |
| id | doaj.art-347ce59aee9a43119de9249a8155d32d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:12:48Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-347ce59aee9a43119de9249a8155d32d2023-11-20T03:36:04ZengMDPI AGPathogens2076-08172020-06-019646410.3390/pathogens9060464Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host ResistanceGuiehdani Villalobos0Claudia I. Muñoz-García1Roberto Rodríguez-Cabo-Mercado2Nancy Mendoza-Bazán3Adrián Hernández-Ortiz4Claudia Villanueva-García5Fernando Martínez-Hernández6Emilio Rendón-Franco7Departamento de Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Calzada de Tlalpan # 4800, Del. Tlalpan, C.P. 14080 Mexico City, MexicoDepartamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana, Calzada del Hueso 1100, Col. Villa Quietud, Del. Coyoacán, C.P. 04960 Mexico City, MexicoIndependent researcher. Siracusa 87, Col. Lomas Estrella, Del. Iztapalapa, C.P. 09890 Mexico City, MexicoDepartamento de Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Calzada de Tlalpan # 4800, Del. Tlalpan, C.P. 14080 Mexico City, MexicoDepartment of Veterinary Microbiology, University of Saskatchewan, 52 Campus Drive, Saskatoon, SK S7N5B4, CanadaLaboratorio de Ecología del Paisaje y Cambio Global, División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, S/N, Rancheria Emiliano Zapata, C.P. 86150 Tabasco, MexicoDepartamento de Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Calzada de Tlalpan # 4800, Del. Tlalpan, C.P. 14080 Mexico City, MexicoDepartamento de Producción Agrícola y Animal, Universidad Autónoma Metropolitana, Calzada del Hueso 1100, Col. Villa Quietud, Del. Coyoacán, C.P. 04960 Mexico City, MexicoMore than 180 mammalian species have been found naturally infected with <i>Trypanosoma cruzi</i>. Many of them play an important role in the maintenance of this parasite. In particular, new studies have appeared which indicate that some species of Procyonidae family may play a role as <i>T. cruzi</i> hosts, however, more data are needed to evaluate their long-term physiological response to parasite infection, especially for specific antibodies. In this study, antibodies to <i>T. cruzi</i> were detected and prevalence and epitope recognition were assessed by ELISA (using discrete typing unit (DTU) I as antigen) and WB (using DTU I and DTU II as antigens) and sera from two procyonid species obtained through five-year follow-up of two semicaptive populations living in the same habitat. Marked heterogeneity in antigens recognition between species and differences in seroprevalence (<i>p</i> = 0.0002) between white-nosed coatis (<i>Nasua narica</i>), 51.8% (115/222), and common raccoons (<i>Procyon lotor</i>), 28.3% (23/81), were found. Antigens with high molecular weight when DTU-I was used were the most recognized, while a greater antigen diversity recognition was observed with DTU-II; for white-nosed coatis, low-molecular-weight antigens were mainly recognized, while for common raccoons proteins with molecular weights greater than 80 kDa were recognized most. These divergent humoral immune responses could be related to an alleged pattern of recognition receptors and major histocompatibility complex molecules difference in the procyonids species.https://www.mdpi.com/2076-0817/9/6/464antibodiesELISA and western blotepitope recognition<i>Nasua narica</i><i>Procyon lotor</i>Procyonidae |
| spellingShingle | Guiehdani Villalobos Claudia I. Muñoz-García Roberto Rodríguez-Cabo-Mercado Nancy Mendoza-Bazán Adrián Hernández-Ortiz Claudia Villanueva-García Fernando Martínez-Hernández Emilio Rendón-Franco Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance Pathogens antibodies ELISA and western blot epitope recognition <i>Nasua narica</i> <i>Procyon lotor</i> Procyonidae |
| title | Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance |
| title_full | Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance |
| title_fullStr | Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance |
| title_full_unstemmed | Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance |
| title_short | Prevalence and Epitope Recognition of Anti-<i>Trypanosoma cruzi</i> Antibodies in Two Procyonid Species: Implications for Host Resistance |
| title_sort | prevalence and epitope recognition of anti i trypanosoma cruzi i antibodies in two procyonid species implications for host resistance |
| topic | antibodies ELISA and western blot epitope recognition <i>Nasua narica</i> <i>Procyon lotor</i> Procyonidae |
| url | https://www.mdpi.com/2076-0817/9/6/464 |
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