The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box
The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is...
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| Format: | Article |
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MDPI AG
2015-01-01
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| Series: | Life |
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| Online Access: | http://www.mdpi.com/2075-1729/5/1/130 |
| _version_ | 1798024185878937600 |
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| author | Paulo Oliveira Nuno M. Martins Marina Santos Narciso A. S. Couto Phillip C. Wright Paula Tamagnini |
| author_facet | Paulo Oliveira Nuno M. Martins Marina Santos Narciso A. S. Couto Phillip C. Wright Paula Tamagnini |
| author_sort | Paulo Oliveira |
| collection | DOAJ |
| description | The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as “hypothetical”, falls into the “other categories” set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins. |
| first_indexed | 2024-04-11T17:58:23Z |
| format | Article |
| id | doaj.art-347dd7fdd84f4191a96b2691660443ea |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-04-11T17:58:23Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-347dd7fdd84f4191a96b2691660443ea2022-12-22T04:10:35ZengMDPI AGLife2075-17292015-01-015113016310.3390/life5010130life5010130The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the BoxPaulo Oliveira0Nuno M. Martins1Marina Santos2Narciso A. S. Couto3Phillip C. Wright4Paula Tamagnini5Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, PortugalInstituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, PortugalInstituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, PortugalChELSI Institute, Chemical and Biological Engineering, University of Sheffield, Sheffield S1 3JD, UKChELSI Institute, Chemical and Biological Engineering, University of Sheffield, Sheffield S1 3JD, UKInstituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, PortugalThe interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as “hypothetical”, falls into the “other categories” set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins.http://www.mdpi.com/2075-1729/5/1/130cyanobacteriaexoproteinprotein secretionmembrane vesicles |
| spellingShingle | Paulo Oliveira Nuno M. Martins Marina Santos Narciso A. S. Couto Phillip C. Wright Paula Tamagnini The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box Life cyanobacteria exoprotein protein secretion membrane vesicles |
| title | The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box |
| title_full | The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box |
| title_fullStr | The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box |
| title_full_unstemmed | The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box |
| title_short | The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box |
| title_sort | anabaena sp pcc 7120 exoproteome taking a peek outside the box |
| topic | cyanobacteria exoprotein protein secretion membrane vesicles |
| url | http://www.mdpi.com/2075-1729/5/1/130 |
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