| Summary: | Lignocellulosic biomass is of great interest as an alternative energy resource because it offers a range of merits. <i>Miscanthus × giganteus</i> is a lignocellulosic feedstock of special interest, as it combines a high biomass productivity with a low environmental impact, including CO<sub>2</sub> emission control. The chemical composition of lignocellulose determines the application potential for efficient industrial processing. Here, we compiled a sample collection of <i>Miscanthus × giganteus</i> that had been cultivated in different climate regions between 2019 and 2021. The chemical composition was quantified by the conventional wet methods. The findings were compared with each other and with the known data. Starting as soon as the first vegetation year, <i>Miscanthus</i> was shown to feature the following chemical composition: 43.2–55.5% cellulose content, 17.1–25.1% acid-insoluble lignin content, 17.9–22.9% pentosan content, 0.90–2.95% ash content, and 0.3–1.2% extractives. The habitat and the surrounding environment were discovered herein to affect the chemical composition of <i>Miscanthus</i>. The stem part of <i>Miscanthus</i> was found to be richer in cellulose than the leaf (48.4–54.9% vs. 47.2–48.9%, respectively), regardless of the planation age and habitat. The obtained findings broaden the investigative geography of the chemical composition of <i>Miscanthus</i> and corroborate the high value of <i>Miscanthus</i> for industrial conversion thereof into cellulosic products worldwide.
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