Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention
3-Monochloropropane-1, 2-diol (3-MCPD), a food-borne contaminant, is widely regarded as the primary cause of male infertility. At present, identifying a method to improve/reduce the male reproductive toxicity caused by 3-MCPD is important. In our study, we explored the potential application of resve...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324000538 |
| _version_ | 1827361001698230272 |
|---|---|
| author | Kai-Lee Wang Yi-Fen Chiang Ko-Chieh Huang Hsin-Yuan Chen Mohamed Ali Shih-Min Hsia |
| author_facet | Kai-Lee Wang Yi-Fen Chiang Ko-Chieh Huang Hsin-Yuan Chen Mohamed Ali Shih-Min Hsia |
| author_sort | Kai-Lee Wang |
| collection | DOAJ |
| description | 3-Monochloropropane-1, 2-diol (3-MCPD), a food-borne contaminant, is widely regarded as the primary cause of male infertility. At present, identifying a method to improve/reduce the male reproductive toxicity caused by 3-MCPD is important. In our study, we explored the potential application of resveratrol (RSV) in mitigating the adverse effects of 3-MCPD. Using 7-week-old Sprague-Dawley (SD) rats as animal models, we investigated the impacts and underlying mechanisms of 3-MCPD and RSV on reproductive function. The administration of 3-MCPD led to significant reductions in testicular and epididymal weights, as well as disruptions in spermatogenesis and histological abnormalities. However, co-treatment with RSV and 3-MCPD mitigated these adverse effects. In vitro study, RSV exhibited the ability to reverse the decline in Leydig and Sertoli cell populations inflicted by 3-MCPD treatment. Mechanistically, RSV reduced endoplasmic reticulum stress (PARP), inflammasome activation (NLRP3), and autophagy-mediated lysosome dysfunction (p62 and LC3BII) induced by 3-MCPD. In addition, 3-MCPD treatment increased the expression level of steroidogenesis-related proteins, steroidogenic acute regulatory (StAR) and CYP11A1, but RSV normalized StAR expression. Moreover, 3-MCPD-induced pro-inflammatory responses were counteracted by RSV treatment, with the cytokine reduction and modulation of CD206 expression, a marker of macrophage activation. These findings indicate that RSV attenuates 3-MCPD-induced reproductive toxicity, highlighting its application potential as an adjuvant agent for male reproductive health. |
| first_indexed | 2024-03-08T06:56:41Z |
| format | Article |
| id | doaj.art-3482d847ad5e4a96a947340ecfb9ac2c |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-08T06:56:41Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-3482d847ad5e4a96a947340ecfb9ac2c2024-02-03T06:34:31ZengElsevierEcotoxicology and Environmental Safety0147-65132024-02-01271115978Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol interventionKai-Lee Wang0Yi-Fen Chiang1Ko-Chieh Huang2Hsin-Yuan Chen3Mohamed Ali4Shih-Min Hsia5Department of Nursing, Deh Yu College of Nursing and Health, Keelung 203301, Taiwan; School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, TaiwanSchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, TaiwanClinical Pharmacy Department, Faculty of Pharmacy, Ain Shams University, 11566 Cairo, Egypt; Department of Obstetrics and Gynecology, University of Chicago, Chicago, IL 60637, USASchool of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, Taiwan; Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, 11031 Taipei, Taiwan; School of Food and Safety, Taipei Medical University, 11031 Taipei, Taiwan; Nutrition Research Center, Taipei Medical University Hospital, 11031 Taipei, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, 11031 Taipei, Taiwan; Correspondence to: School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan.3-Monochloropropane-1, 2-diol (3-MCPD), a food-borne contaminant, is widely regarded as the primary cause of male infertility. At present, identifying a method to improve/reduce the male reproductive toxicity caused by 3-MCPD is important. In our study, we explored the potential application of resveratrol (RSV) in mitigating the adverse effects of 3-MCPD. Using 7-week-old Sprague-Dawley (SD) rats as animal models, we investigated the impacts and underlying mechanisms of 3-MCPD and RSV on reproductive function. The administration of 3-MCPD led to significant reductions in testicular and epididymal weights, as well as disruptions in spermatogenesis and histological abnormalities. However, co-treatment with RSV and 3-MCPD mitigated these adverse effects. In vitro study, RSV exhibited the ability to reverse the decline in Leydig and Sertoli cell populations inflicted by 3-MCPD treatment. Mechanistically, RSV reduced endoplasmic reticulum stress (PARP), inflammasome activation (NLRP3), and autophagy-mediated lysosome dysfunction (p62 and LC3BII) induced by 3-MCPD. In addition, 3-MCPD treatment increased the expression level of steroidogenesis-related proteins, steroidogenic acute regulatory (StAR) and CYP11A1, but RSV normalized StAR expression. Moreover, 3-MCPD-induced pro-inflammatory responses were counteracted by RSV treatment, with the cytokine reduction and modulation of CD206 expression, a marker of macrophage activation. These findings indicate that RSV attenuates 3-MCPD-induced reproductive toxicity, highlighting its application potential as an adjuvant agent for male reproductive health.http://www.sciencedirect.com/science/article/pii/S0147651324000538TestosteroneLeydig cellsSteroidogenesis3-MCPD |
| spellingShingle | Kai-Lee Wang Yi-Fen Chiang Ko-Chieh Huang Hsin-Yuan Chen Mohamed Ali Shih-Min Hsia Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention Ecotoxicology and Environmental Safety Testosterone Leydig cells Steroidogenesis 3-MCPD |
| title | Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention |
| title_full | Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention |
| title_fullStr | Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention |
| title_full_unstemmed | Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention |
| title_short | Alleviating 3-MCPD-induced male reproductive toxicity: Mechanistic insights and resveratrol intervention |
| title_sort | alleviating 3 mcpd induced male reproductive toxicity mechanistic insights and resveratrol intervention |
| topic | Testosterone Leydig cells Steroidogenesis 3-MCPD |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324000538 |
| work_keys_str_mv | AT kaileewang alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention AT yifenchiang alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention AT kochiehhuang alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention AT hsinyuanchen alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention AT mohamedali alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention AT shihminhsia alleviating3mcpdinducedmalereproductivetoxicitymechanisticinsightsandresveratrolintervention |