Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors
Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/13/8/1258 |
| _version_ | 1797522346138927104 |
|---|---|
| author | Nuria Arias-Ramos Luis Exequiel Ibarra María Serrano-Torres Balbino Yagüe Matías Daniel Caverzán Carlos Alberto Chesta Rodrigo Emiliano Palacios Pilar López-Larrubia |
| author_facet | Nuria Arias-Ramos Luis Exequiel Ibarra María Serrano-Torres Balbino Yagüe Matías Daniel Caverzán Carlos Alberto Chesta Rodrigo Emiliano Palacios Pilar López-Larrubia |
| author_sort | Nuria Arias-Ramos |
| collection | DOAJ |
| description | Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functionalization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe<sub>3</sub>O<sub>4</sub> and NiFe<sub>2</sub>O<sub>4</sub> nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T<sub>2</sub>WI) signals and T<sub>2</sub> values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles. |
| first_indexed | 2024-03-10T08:28:07Z |
| format | Article |
| id | doaj.art-348571f5d5b241ab83fdca4ac0f81c58 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:28:07Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-348571f5d5b241ab83fdca4ac0f81c582023-11-22T09:15:06ZengMDPI AGPharmaceutics1999-49232021-08-01138125810.3390/pharmaceutics13081258Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain TumorsNuria Arias-Ramos0Luis Exequiel Ibarra1María Serrano-Torres2Balbino Yagüe3Matías Daniel Caverzán4Carlos Alberto Chesta5Rodrigo Emiliano Palacios6Pilar López-Larrubia7Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 28029 Madrid, SpainInstituto de Biotecnología Ambiental y Salud (INBIAS), Universidad Nacional de Río Cuarto (UNRC) y CONICET, Córdoba X5800BIA, ArgentinaInstituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 28029 Madrid, SpainInstituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 28029 Madrid, SpainDepartamento de Biología Molecular, Facultad de Ciencias Exactas, Fisicoquímicas y Naturales, Universidad Nacional de Río Cuarto (UNRC), Córdoba X5800BIA, ArgentinaInstituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados (IITEMA), Universidad Nacional de Río Cuarto y CONICET, Córdoba X5800BIA, ArgentinaInstituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados (IITEMA), Universidad Nacional de Río Cuarto y CONICET, Córdoba X5800BIA, ArgentinaInstituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 28029 Madrid, SpainConjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functionalization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe<sub>3</sub>O<sub>4</sub> and NiFe<sub>2</sub>O<sub>4</sub> nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T<sub>2</sub>WI) signals and T<sub>2</sub> values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles.https://www.mdpi.com/1999-4923/13/8/1258theranosticconjugated polymeriron oxide nanoparticlesglioblastomapreclinical trialsnanoparticles |
| spellingShingle | Nuria Arias-Ramos Luis Exequiel Ibarra María Serrano-Torres Balbino Yagüe Matías Daniel Caverzán Carlos Alberto Chesta Rodrigo Emiliano Palacios Pilar López-Larrubia Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors Pharmaceutics theranostic conjugated polymer iron oxide nanoparticles glioblastoma preclinical trials nanoparticles |
| title | Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors |
| title_full | Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors |
| title_fullStr | Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors |
| title_full_unstemmed | Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors |
| title_short | Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors |
| title_sort | iron oxide incorporated conjugated polymer nanoparticles for simultaneous use in magnetic resonance and fluorescent imaging of brain tumors |
| topic | theranostic conjugated polymer iron oxide nanoparticles glioblastoma preclinical trials nanoparticles |
| url | https://www.mdpi.com/1999-4923/13/8/1258 |
| work_keys_str_mv | AT nuriaariasramos ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT luisexequielibarra ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT mariaserranotorres ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT balbinoyague ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT matiasdanielcaverzan ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT carlosalbertochesta ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT rodrigoemilianopalacios ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors AT pilarlopezlarrubia ironoxideincorporatedconjugatedpolymernanoparticlesforsimultaneoususeinmagneticresonanceandfluorescentimagingofbraintumors |