Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines
DNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), and hyaluronic acid (HA) to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-02-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/7/2/30 |
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| author | Fatin M. Nawwab Al-Deen Sue D. Xiang Charles Ma Kirsty Wilson Ross L. Coppel Cordelia Selomulya Magdalena Plebanski |
| author_facet | Fatin M. Nawwab Al-Deen Sue D. Xiang Charles Ma Kirsty Wilson Ross L. Coppel Cordelia Selomulya Magdalena Plebanski |
| author_sort | Fatin M. Nawwab Al-Deen |
| collection | DOAJ |
| description | DNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), and hyaluronic acid (HA) to deliver malaria DNA encoding Plasmodium yoelii (Py) merozoite surface protein MSP119 (SPIONs/PEI/DNA + HA gene complex) to dendritic cells and transfect them with high efficiency in vitro. Herein, we evaluate their immunogenicity in vivo by administering these potential vaccine complexes into BALB/c mice. The complexes induced antibodies against PyMSP119, with higher responses induced intraperitoneally than intramuscularly, and antibody levels further enhanced by applying an external magnetic field. The predominant IgG subclasses induced were IgG2a followed by IgG1 and IgG2b. The complexes further elicited high levels of interferon gamma (IFN-γ), and moderate levels of interleukin (IL)-4 and IL-17 antigen-specific splenocytes, indicating induction of T helper 1 (Th1), Th2, and Th17 cell mediated immunity. The ability of such DNA/nanoparticle complexes to induce cytophilic antibodies together with broad spectrum cellular immunity may benefit malaria vaccines. |
| first_indexed | 2024-12-20T03:25:20Z |
| format | Article |
| id | doaj.art-3486928d52084d3381a6f2d58d7b9620 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T03:25:20Z |
| publishDate | 2017-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-3486928d52084d3381a6f2d58d7b96202022-12-21T19:55:07ZengMDPI AGNanomaterials2079-49912017-02-01723010.3390/nano7020030nano7020030Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria VaccinesFatin M. Nawwab Al-Deen0Sue D. Xiang1Charles Ma2Kirsty Wilson3Ross L. Coppel4Cordelia Selomulya5Magdalena Plebanski6Department of Chemical Engineering, Monash University, 18 Alliance Lane, Clayton, VIC 3800, AustraliaDepartment of Immunology and Pathology, Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, 89 Commercial Road, Melbourne, VIC 3004, AustraliaDepartment of Microbiology, Monash University, Wellington Road, Clayton, VIC 3800, AustraliaDepartment of Immunology and Pathology, Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, 89 Commercial Road, Melbourne, VIC 3004, AustraliaDepartment of Microbiology, Monash University, Wellington Road, Clayton, VIC 3800, AustraliaDepartment of Chemical Engineering, Monash University, 18 Alliance Lane, Clayton, VIC 3800, AustraliaDepartment of Immunology and Pathology, Central Clinical School, Faculty of Medicine, Nursing and Health Sciences, Monash University, 89 Commercial Road, Melbourne, VIC 3004, AustraliaDNA vaccines offer cost, flexibility, and stability advantages, but administered alone have limited immunogenicity. Previously, we identified optimal configurations of magnetic vectors comprising superparamagnetic iron oxide nanoparticles (SPIONs), polyethylenimine (PEI), and hyaluronic acid (HA) to deliver malaria DNA encoding Plasmodium yoelii (Py) merozoite surface protein MSP119 (SPIONs/PEI/DNA + HA gene complex) to dendritic cells and transfect them with high efficiency in vitro. Herein, we evaluate their immunogenicity in vivo by administering these potential vaccine complexes into BALB/c mice. The complexes induced antibodies against PyMSP119, with higher responses induced intraperitoneally than intramuscularly, and antibody levels further enhanced by applying an external magnetic field. The predominant IgG subclasses induced were IgG2a followed by IgG1 and IgG2b. The complexes further elicited high levels of interferon gamma (IFN-γ), and moderate levels of interleukin (IL)-4 and IL-17 antigen-specific splenocytes, indicating induction of T helper 1 (Th1), Th2, and Th17 cell mediated immunity. The ability of such DNA/nanoparticle complexes to induce cytophilic antibodies together with broad spectrum cellular immunity may benefit malaria vaccines.http://www.mdpi.com/2079-4991/7/2/30hyaluronic acidMSP119superparamagnetic iron oxide nanoparticles (SPIONs)magnetic gene vectormalaria DNA vaccineantibodyimmune response |
| spellingShingle | Fatin M. Nawwab Al-Deen Sue D. Xiang Charles Ma Kirsty Wilson Ross L. Coppel Cordelia Selomulya Magdalena Plebanski Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines Nanomaterials hyaluronic acid MSP119 superparamagnetic iron oxide nanoparticles (SPIONs) magnetic gene vector malaria DNA vaccine antibody immune response |
| title | Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines |
| title_full | Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines |
| title_fullStr | Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines |
| title_full_unstemmed | Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines |
| title_short | Magnetic Nanovectors for the Development of DNA Blood-Stage Malaria Vaccines |
| title_sort | magnetic nanovectors for the development of dna blood stage malaria vaccines |
| topic | hyaluronic acid MSP119 superparamagnetic iron oxide nanoparticles (SPIONs) magnetic gene vector malaria DNA vaccine antibody immune response |
| url | http://www.mdpi.com/2079-4991/7/2/30 |
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