Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway
A synthetic dibenzothiophene (DBT) mineralization pathway has been engineered in recombinant cells of Pseudomonas azelaica Aramco J strain for its use in biodesulfurization of thiophenic compounds and crude oil. This functional pathway consists of a combination of a recombinant 4S pathway responsibl...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.987084/full |
| _version_ | 1811204841341452288 |
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| author | Igor Martínez Magdy El-Said Mohamed José Luis García Eduardo Díaz |
| author_facet | Igor Martínez Magdy El-Said Mohamed José Luis García Eduardo Díaz |
| author_sort | Igor Martínez |
| collection | DOAJ |
| description | A synthetic dibenzothiophene (DBT) mineralization pathway has been engineered in recombinant cells of Pseudomonas azelaica Aramco J strain for its use in biodesulfurization of thiophenic compounds and crude oil. This functional pathway consists of a combination of a recombinant 4S pathway responsible for the conversion of DBT into 2-hydroxybiphenyl (2HBP) and a 2HBP mineralization pathway that is naturally present in the parental P. azelaica Aramco J strain. This novel approach allows overcoming one of the major bottlenecks of the biodesulfurization process, i.e., the feedback inhibitory effect of 2HBP on the 4S pathway enzymes. Resting cells-based biodesulfurization assays using DBT as a sulfur source showed that the 2HBP generated from the 4S pathway is subsequently metabolized by the cell, yielding an increase of 100% in DBT removal with respect to previously optimized Pseudomonas putida biodesulfurizing strains. Moreover, the recombinant P. azelaica Aramco J strain was able to use DBT as a carbon source, representing the best characterized biocatalyst harboring a DBT mineralization pathway and constituting a suitable candidate to develop future bioremediation/bioconversion strategies for oil-contaminated sites. |
| first_indexed | 2024-04-12T03:20:31Z |
| format | Article |
| id | doaj.art-348731744e5d4aa586bb49ecd1119b27 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T03:20:31Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-348731744e5d4aa586bb49ecd1119b272022-12-22T03:49:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.987084987084Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathwayIgor Martínez0Magdy El-Said Mohamed1José Luis García2Eduardo Díaz3Department of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas-Consejo Superior de Investigaciones Científicas, Madrid, SpainResearch and Development Center, Saudi Aramco, Dhahran, Saudi ArabiaDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas-Consejo Superior de Investigaciones Científicas, Madrid, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas-Consejo Superior de Investigaciones Científicas, Madrid, SpainA synthetic dibenzothiophene (DBT) mineralization pathway has been engineered in recombinant cells of Pseudomonas azelaica Aramco J strain for its use in biodesulfurization of thiophenic compounds and crude oil. This functional pathway consists of a combination of a recombinant 4S pathway responsible for the conversion of DBT into 2-hydroxybiphenyl (2HBP) and a 2HBP mineralization pathway that is naturally present in the parental P. azelaica Aramco J strain. This novel approach allows overcoming one of the major bottlenecks of the biodesulfurization process, i.e., the feedback inhibitory effect of 2HBP on the 4S pathway enzymes. Resting cells-based biodesulfurization assays using DBT as a sulfur source showed that the 2HBP generated from the 4S pathway is subsequently metabolized by the cell, yielding an increase of 100% in DBT removal with respect to previously optimized Pseudomonas putida biodesulfurizing strains. Moreover, the recombinant P. azelaica Aramco J strain was able to use DBT as a carbon source, representing the best characterized biocatalyst harboring a DBT mineralization pathway and constituting a suitable candidate to develop future bioremediation/bioconversion strategies for oil-contaminated sites.https://www.frontiersin.org/articles/10.3389/fmicb.2022.987084/fullPseudomonas azelaicadibenzothiophenemetabolic engineeringbiodesulfurization4S pathway |
| spellingShingle | Igor Martínez Magdy El-Said Mohamed José Luis García Eduardo Díaz Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway Frontiers in Microbiology Pseudomonas azelaica dibenzothiophene metabolic engineering biodesulfurization 4S pathway |
| title | Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| title_full | Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| title_fullStr | Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| title_full_unstemmed | Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| title_short | Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| title_sort | enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway |
| topic | Pseudomonas azelaica dibenzothiophene metabolic engineering biodesulfurization 4S pathway |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.987084/full |
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