Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice
The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of <i>OsPIN9</i> leads to altered plant architecture and chilling tolerance in rice. The expression profile anal...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/15/2809 |
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| author | Qiqi Ouyang Yanwen Zhang Xiaoyi Yang Chong Yang Dianyun Hou Hao Liu Huawei Xu |
| author_facet | Qiqi Ouyang Yanwen Zhang Xiaoyi Yang Chong Yang Dianyun Hou Hao Liu Huawei Xu |
| author_sort | Qiqi Ouyang |
| collection | DOAJ |
| description | The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of <i>OsPIN9</i> leads to altered plant architecture and chilling tolerance in rice. The expression profile analysis indicated that <i>OsPIN9</i> was gradually suppressed by chilling stress. The shoot height and adventitious root number of <i>OsPIN9-</i>overexpressing (OE) plants were significantly reduced at the seedling stage. The roots of OE plants were more tolerant to <i>N</i>-1-naphthylphthalamic acid (NPA) treatment than WT plants, indicating the disturbance of auxin homeostasis in OE lines. The chilling tolerance assay showed that the survival rate of OE plants was markedly lower than that of wild-type (WT) plants. Consistently, more dead cells, increased electrolyte leakage, and increased malondialdehyde (MDA) content were observed in OE plants compared to those in WT plants under chilling conditions. Notably, OE plants accumulated more hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and less superoxide anion radicals (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi mathvariant="normal">O</mi></mrow><mrow><mn>2</mn></mrow><mrow><mo>−</mo></mrow></msubsup></mrow></semantics></math></inline-formula>) than WT plants under chilling conditions. In contrast, catalase (CAT) and superoxide dismutase (SOD) activities in OE lines decreased significantly compared to those in WT plants at the early chilling stage, implying that the impaired chilling tolerance of transgenic plants is probably attributed to the sharp induction of H<sub>2</sub>O<sub>2</sub> and the delayed induction of antioxidant enzyme activities at this stage. In addition, several <i>OsRboh</i> genes, which play a crucial role in ROS production under abiotic stress, showed an obvious increase after chilling stress in OE plants compared to that in WT plants, which probably at least in part contributes to the production of ROS under chilling stress in OE plants. Together, our results reveal that <i>OsPIN9</i> plays a vital role in regulating plant architecture and, more importantly, is involved in regulating rice chilling tolerance by influencing auxin and ROS homeostasis. |
| first_indexed | 2024-03-11T00:18:14Z |
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| language | English |
| last_indexed | 2024-03-11T00:18:14Z |
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| spelling | doaj.art-34888ed2cec14ebeae716065e359f0e72023-11-18T23:26:10ZengMDPI AGPlants2223-77472023-07-011215280910.3390/plants12152809Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in RiceQiqi Ouyang0Yanwen Zhang1Xiaoyi Yang2Chong Yang3Dianyun Hou4Hao Liu5Huawei Xu6College of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Agriculture, Henan University of Science and Technology, Luoyang 471000, ChinaThe auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of <i>OsPIN9</i> leads to altered plant architecture and chilling tolerance in rice. The expression profile analysis indicated that <i>OsPIN9</i> was gradually suppressed by chilling stress. The shoot height and adventitious root number of <i>OsPIN9-</i>overexpressing (OE) plants were significantly reduced at the seedling stage. The roots of OE plants were more tolerant to <i>N</i>-1-naphthylphthalamic acid (NPA) treatment than WT plants, indicating the disturbance of auxin homeostasis in OE lines. The chilling tolerance assay showed that the survival rate of OE plants was markedly lower than that of wild-type (WT) plants. Consistently, more dead cells, increased electrolyte leakage, and increased malondialdehyde (MDA) content were observed in OE plants compared to those in WT plants under chilling conditions. Notably, OE plants accumulated more hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and less superoxide anion radicals (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msubsup><mrow><mi mathvariant="normal">O</mi></mrow><mrow><mn>2</mn></mrow><mrow><mo>−</mo></mrow></msubsup></mrow></semantics></math></inline-formula>) than WT plants under chilling conditions. In contrast, catalase (CAT) and superoxide dismutase (SOD) activities in OE lines decreased significantly compared to those in WT plants at the early chilling stage, implying that the impaired chilling tolerance of transgenic plants is probably attributed to the sharp induction of H<sub>2</sub>O<sub>2</sub> and the delayed induction of antioxidant enzyme activities at this stage. In addition, several <i>OsRboh</i> genes, which play a crucial role in ROS production under abiotic stress, showed an obvious increase after chilling stress in OE plants compared to that in WT plants, which probably at least in part contributes to the production of ROS under chilling stress in OE plants. Together, our results reveal that <i>OsPIN9</i> plays a vital role in regulating plant architecture and, more importantly, is involved in regulating rice chilling tolerance by influencing auxin and ROS homeostasis.https://www.mdpi.com/2223-7747/12/15/2809<i>OsPIN9</i>polar auxin transportchilling toleranceROS homeostasisantioxidant enzymesrice (<i>Oryza sativa</i> L.) |
| spellingShingle | Qiqi Ouyang Yanwen Zhang Xiaoyi Yang Chong Yang Dianyun Hou Hao Liu Huawei Xu Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice Plants <i>OsPIN9</i> polar auxin transport chilling tolerance ROS homeostasis antioxidant enzymes rice (<i>Oryza sativa</i> L.) |
| title | Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice |
| title_full | Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice |
| title_fullStr | Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice |
| title_full_unstemmed | Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice |
| title_short | Overexpression of <i>OsPIN9</i> Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice |
| title_sort | overexpression of i ospin9 i impairs chilling tolerance via disturbing ros homeostasis in rice |
| topic | <i>OsPIN9</i> polar auxin transport chilling tolerance ROS homeostasis antioxidant enzymes rice (<i>Oryza sativa</i> L.) |
| url | https://www.mdpi.com/2223-7747/12/15/2809 |
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