The metabolism of 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid into cholic: an enzyme assay using homogenates of human liver
An enzyme assay was developed to measure the conversion of the bile acid precursor, 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 ± 0.5 (± 1 SD) pmoles of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1976-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752036990X |
| Summary: | An enzyme assay was developed to measure the conversion of the bile acid precursor, 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 ± 0.5 (± 1 SD) pmoles of cholic acid formed/mg liver/minute in twelve normal liver biopsies. This assay system can be used to determine if the syndrome of neonatal cholestasis associated with a metabolic block in the conversion of THCA into cholic acid is transmitted as a genetic trait. |
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| ISSN: | 0022-2275 |