Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy
<p>Abstract</p> <p>Gene expression analysis has proven to be a very useful tool to gain knowledge of the factors involved in the pathogenesis of diseases, particularly in the initial or preclinical stages. With the aim of finding new data on the events occurring in the Central Nerv...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-10-01
|
| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/109 |
| _version_ | 1811266752526417920 |
|---|---|
| author | Tortosa Raül Castells Xavier Vidal Enric Costa Carme Ruiz de Villa María Sánchez Àlex Barceló Anna Torres Juan Pumarola Martí Ariño Joaquín |
| author_facet | Tortosa Raül Castells Xavier Vidal Enric Costa Carme Ruiz de Villa María Sánchez Àlex Barceló Anna Torres Juan Pumarola Martí Ariño Joaquín |
| author_sort | Tortosa Raül |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Gene expression analysis has proven to be a very useful tool to gain knowledge of the factors involved in the pathogenesis of diseases, particularly in the initial or preclinical stages. With the aim of finding new data on the events occurring in the Central Nervous System in animals affected with Bovine Spongiform Encephalopathy, a comprehensive genome wide gene expression study was conducted at different time points of the disease on mice genetically modified to model the bovine species brain in terms of cellular prion protein. An accurate analysis of the information generated by microarray technique was the key point to assess the biological relevance of the data obtained in terms of Transmissible Spongiform Encephalopathy pathogenesis. Validation of the microarray technique was achieved by RT-PCR confirming the RNA change and immunohistochemistry techniques that verified that expression changes were translated into variable levels of protein for selected genes. Our study reveals changes in the expression of genes, some of them not previously associated with prion diseases, at early stages of the disease previous to the detection of the pathological prion protein, that might have a role in neuronal degeneration and several transcriptional changes showing an important imbalance in the Central Nervous System homeostasis in advanced stages of the disease. Genes whose expression is altered at early stages of the disease should be considered as possible therapeutic targets and potential disease markers in preclinical diagnostic tool development. Genes non-previously related to prion diseases should be taken into consideration for further investigations.</p> |
| first_indexed | 2024-04-12T20:48:58Z |
| format | Article |
| id | doaj.art-348a0ffbbc654d23b33a1543764136f2 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-04-12T20:48:58Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-348a0ffbbc654d23b33a1543764136f22022-12-22T03:17:10ZengBMCVeterinary Research0928-42491297-97162011-10-0142110910.1186/1297-9716-42-109Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathyTortosa RaülCastells XavierVidal EnricCosta CarmeRuiz de Villa MaríaSánchez ÀlexBarceló AnnaTorres JuanPumarola MartíAriño Joaquín<p>Abstract</p> <p>Gene expression analysis has proven to be a very useful tool to gain knowledge of the factors involved in the pathogenesis of diseases, particularly in the initial or preclinical stages. With the aim of finding new data on the events occurring in the Central Nervous System in animals affected with Bovine Spongiform Encephalopathy, a comprehensive genome wide gene expression study was conducted at different time points of the disease on mice genetically modified to model the bovine species brain in terms of cellular prion protein. An accurate analysis of the information generated by microarray technique was the key point to assess the biological relevance of the data obtained in terms of Transmissible Spongiform Encephalopathy pathogenesis. Validation of the microarray technique was achieved by RT-PCR confirming the RNA change and immunohistochemistry techniques that verified that expression changes were translated into variable levels of protein for selected genes. Our study reveals changes in the expression of genes, some of them not previously associated with prion diseases, at early stages of the disease previous to the detection of the pathological prion protein, that might have a role in neuronal degeneration and several transcriptional changes showing an important imbalance in the Central Nervous System homeostasis in advanced stages of the disease. Genes whose expression is altered at early stages of the disease should be considered as possible therapeutic targets and potential disease markers in preclinical diagnostic tool development. Genes non-previously related to prion diseases should be taken into consideration for further investigations.</p>http://www.veterinaryresearch.org/content/42/1/109 |
| spellingShingle | Tortosa Raül Castells Xavier Vidal Enric Costa Carme Ruiz de Villa María Sánchez Àlex Barceló Anna Torres Juan Pumarola Martí Ariño Joaquín Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy Veterinary Research |
| title | Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| title_full | Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| title_fullStr | Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| title_full_unstemmed | Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| title_short | Central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| title_sort | central nervous system gene expression changes in a transgenic mouse model for bovine spongiform encephalopathy |
| url | http://www.veterinaryresearch.org/content/42/1/109 |
| work_keys_str_mv | AT tortosaraul centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT castellsxavier centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT vidalenric centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT costacarme centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT ruizdevillamaria centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT sanchezalex centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT barceloanna centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT torresjuan centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT pumarolamarti centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy AT arinojoaquin centralnervoussystemgeneexpressionchangesinatransgenicmousemodelforbovinespongiformencephalopathy |