Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice
The intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/19/8/456 |
| _version_ | 1827684845832110080 |
|---|---|
| author | Xing-Wei Xiang Hui-Zhen Zheng Rui Wang Hui Chen Jin-Xing Xiao Bin Zheng Shu-Lai Liu Yu-Ting Ding |
| author_facet | Xing-Wei Xiang Hui-Zhen Zheng Rui Wang Hui Chen Jin-Xing Xiao Bin Zheng Shu-Lai Liu Yu-Ting Ding |
| author_sort | Xing-Wei Xiang |
| collection | DOAJ |
| description | The intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored the indexes of thymus, spleen and liver, stimulated cytokines secretion and promoted the relative mRNA levels of Th1/Th2 cytokines (IL-2, IFN-γ, IL-4 and IL-10). The mRNA levels of Occludin, Claudin-1, ZO-1, and Mucin-2 were up-regulated, and the NF-κB signaling pathway was also activated after oyster peptides administration. Furthermore, oyster peptides treatment reduced the proportion of <i>Firmicutes</i>/<i>Bacteroidetes</i>, increased the relative abundance of <i>Alistipes</i>, <i>Lactobacillus</i>, <i>Rikenell</i> and the content of short-chain fatty acids, and reversed the composition of intestinal microflora similar to that of normal mice. In conclusion, oyster peptides effectively ameliorated cyclophosphamide-induced intestinal damage and modified gut microbiota structure in mice, and might be utilized as a beneficial ingredient in functional foods for immune regulation. |
| first_indexed | 2024-03-10T08:38:54Z |
| format | Article |
| id | doaj.art-348c21a1e4e2409498114f1d952a2364 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T08:38:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-348c21a1e4e2409498114f1d952a23642023-11-22T08:25:58ZengMDPI AGMarine Drugs1660-33972021-08-0119845610.3390/md19080456Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated MiceXing-Wei Xiang0Hui-Zhen Zheng1Rui Wang2Hui Chen3Jin-Xing Xiao4Bin Zheng5Shu-Lai Liu6Yu-Ting Ding7College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaOcean Research Center of Zhoushan, Zhejiang University, Zhoushan 316000, ChinaFood and Pharmacy College, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, ChinaThe intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored the indexes of thymus, spleen and liver, stimulated cytokines secretion and promoted the relative mRNA levels of Th1/Th2 cytokines (IL-2, IFN-γ, IL-4 and IL-10). The mRNA levels of Occludin, Claudin-1, ZO-1, and Mucin-2 were up-regulated, and the NF-κB signaling pathway was also activated after oyster peptides administration. Furthermore, oyster peptides treatment reduced the proportion of <i>Firmicutes</i>/<i>Bacteroidetes</i>, increased the relative abundance of <i>Alistipes</i>, <i>Lactobacillus</i>, <i>Rikenell</i> and the content of short-chain fatty acids, and reversed the composition of intestinal microflora similar to that of normal mice. In conclusion, oyster peptides effectively ameliorated cyclophosphamide-induced intestinal damage and modified gut microbiota structure in mice, and might be utilized as a beneficial ingredient in functional foods for immune regulation.https://www.mdpi.com/1660-3397/19/8/456oyster (<i>Crassostrea gigas</i>)peptidescyclophosphamideimmunomodulatorygut microbiotashort-chain fatty acid |
| spellingShingle | Xing-Wei Xiang Hui-Zhen Zheng Rui Wang Hui Chen Jin-Xing Xiao Bin Zheng Shu-Lai Liu Yu-Ting Ding Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice Marine Drugs oyster (<i>Crassostrea gigas</i>) peptides cyclophosphamide immunomodulatory gut microbiota short-chain fatty acid |
| title | Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice |
| title_full | Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice |
| title_fullStr | Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice |
| title_full_unstemmed | Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice |
| title_short | Ameliorative Effects of Peptides Derived from Oyster (<i>Crassostrea gigas</i>) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice |
| title_sort | ameliorative effects of peptides derived from oyster i crassostrea gigas i on immunomodulatory function and gut microbiota structure in cyclophosphamide treated mice |
| topic | oyster (<i>Crassostrea gigas</i>) peptides cyclophosphamide immunomodulatory gut microbiota short-chain fatty acid |
| url | https://www.mdpi.com/1660-3397/19/8/456 |
| work_keys_str_mv | AT xingweixiang ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT huizhenzheng ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT ruiwang ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT huichen ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT jinxingxiao ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT binzheng ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT shulailiu ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice AT yutingding ameliorativeeffectsofpeptidesderivedfromoystericrassostreagigasionimmunomodulatoryfunctionandgutmicrobiotastructureincyclophosphamidetreatedmice |