Treadmill-based task for assessing spatial memory in head-fixed mice
Summary: Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cue...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004767 |
| _version_ | 1819082296374853632 |
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| author | Jake T. Jordan Kelsey D. McDermott M. Agustina Frechou Matthew Shtrahman J. Tiago Gonçalves |
| author_facet | Jake T. Jordan Kelsey D. McDermott M. Agustina Frechou Matthew Shtrahman J. Tiago Gonçalves |
| author_sort | Jake T. Jordan |
| collection | DOAJ |
| description | Summary: Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task. |
| first_indexed | 2024-12-21T20:14:25Z |
| format | Article |
| id | doaj.art-348e5aa7ed7e40f9a7b573f84653ff07 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T20:14:25Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-348e5aa7ed7e40f9a7b573f84653ff072022-12-21T18:51:40ZengElsevierSTAR Protocols2666-16672021-09-0123100770Treadmill-based task for assessing spatial memory in head-fixed miceJake T. Jordan0Kelsey D. McDermott1M. Agustina Frechou2Matthew Shtrahman3J. Tiago Gonçalves4Dominick P. Purpura Department of Neuroscience and Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Corresponding authorDominick P. Purpura Department of Neuroscience and Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USADominick P. Purpura Department of Neuroscience and Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USADepartment of Neurosciences, University of California San Diego, La Jolla, CA 92093, USADominick P. Purpura Department of Neuroscience and Gottesman Institute for Stem Cell Biology and Regenerative Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Corresponding authorSummary: Several mouse in vivo neuronal recording techniques require head fixation. Head-fixed treadmill walking can be used to design tasks that enable the study of neural activity in the context of behavior. Here, we provide a detailed protocol for constructing a treadmill with tactile spatial cues, training mice on a rewarded behavioral task, and analyzing behavioral data. We discuss common problems and solutions we have developed to optimize training. Finally, we demonstrate how to test spatial memory performance using this task.http://www.sciencedirect.com/science/article/pii/S2666166721004767MicroscopyModel OrganismsNeuroscienceBehavior |
| spellingShingle | Jake T. Jordan Kelsey D. McDermott M. Agustina Frechou Matthew Shtrahman J. Tiago Gonçalves Treadmill-based task for assessing spatial memory in head-fixed mice STAR Protocols Microscopy Model Organisms Neuroscience Behavior |
| title | Treadmill-based task for assessing spatial memory in head-fixed mice |
| title_full | Treadmill-based task for assessing spatial memory in head-fixed mice |
| title_fullStr | Treadmill-based task for assessing spatial memory in head-fixed mice |
| title_full_unstemmed | Treadmill-based task for assessing spatial memory in head-fixed mice |
| title_short | Treadmill-based task for assessing spatial memory in head-fixed mice |
| title_sort | treadmill based task for assessing spatial memory in head fixed mice |
| topic | Microscopy Model Organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004767 |
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