Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow
Laser ektacytometry is a technique widely used for measuring the deformability of red blood cells (erythrocytes) in blood samples in vitro. In ektacytometer, a flow of highly diluted suspension of erythrocytes in variable shear stress conditions is illuminated with a laser beam to obtain a diffracti...
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2015-07-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500315 |
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| author | S. Yu. Nikitin A. E. Lugovtsov V. D. Ustinov M. D. Lin A. V. Priezzhev |
| author_facet | S. Yu. Nikitin A. E. Lugovtsov V. D. Ustinov M. D. Lin A. V. Priezzhev |
| author_sort | S. Yu. Nikitin |
| collection | DOAJ |
| description | Laser ektacytometry is a technique widely used for measuring the deformability of red blood cells (erythrocytes) in blood samples in vitro. In ektacytometer, a flow of highly diluted suspension of erythrocytes in variable shear stress conditions is illuminated with a laser beam to obtain a diffraction pattern. The diffraction pattern provides information about the shapes (shear-induced elongations) of the cells under investigation. This paper is dedicated to developing the technique of laser ektacytometry so that it would enable one to measure the distribution of the erythrocytes in deformability. We discuss the problem of calibration of laser ektacytometer and test a novel data processing algorithm allowing to determine the parameters of the distribution of erythrocytes deformability. Experimentally, we examined 12 specimens of blood of rats under the action of 4 shear stresses. Analysis of the data shows that in conditions of a limited range of digitizing the diffraction patterns, the measurement errors for the mean deformability, deformability scatter and the skewness of erythrocytes distribution in deformability by our method are respectively 15%, 20% and 20%. |
| first_indexed | 2024-12-17T07:34:44Z |
| format | Article |
| id | doaj.art-348fe111fea44b72b04c8de34c66ece9 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-17T07:34:44Z |
| publishDate | 2015-07-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-348fe111fea44b72b04c8de34c66ece92022-12-21T21:58:22ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052015-07-01841550031-11550031-1210.1142/S179354581550031510.1142/S1793545815500315Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flowS. Yu. Nikitin0A. E. Lugovtsov1V. D. Ustinov2M. D. Lin3A. V. Priezzhev4Physics Faculty Lomonosov Moscow State University, Moscow, RussiaInternational Laser Center Lomonosov Moscow, State University, Moscow, RussiaFaculty of Computational Mathematics and Cybernetics Lomonosov Moscow State University, Moscow, RussiaFaculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, RussiaPhysics Faculty Lomonosov Moscow State University, Moscow, RussiaLaser ektacytometry is a technique widely used for measuring the deformability of red blood cells (erythrocytes) in blood samples in vitro. In ektacytometer, a flow of highly diluted suspension of erythrocytes in variable shear stress conditions is illuminated with a laser beam to obtain a diffraction pattern. The diffraction pattern provides information about the shapes (shear-induced elongations) of the cells under investigation. This paper is dedicated to developing the technique of laser ektacytometry so that it would enable one to measure the distribution of the erythrocytes in deformability. We discuss the problem of calibration of laser ektacytometer and test a novel data processing algorithm allowing to determine the parameters of the distribution of erythrocytes deformability. Experimentally, we examined 12 specimens of blood of rats under the action of 4 shear stresses. Analysis of the data shows that in conditions of a limited range of digitizing the diffraction patterns, the measurement errors for the mean deformability, deformability scatter and the skewness of erythrocytes distribution in deformability by our method are respectively 15%, 20% and 20%.http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500315Laser diffractometryektacytometryshear flowdiffraction patterndeformability of erythrocyteserythrocytes variance in shapesimage analysis |
| spellingShingle | S. Yu. Nikitin A. E. Lugovtsov V. D. Ustinov M. D. Lin A. V. Priezzhev Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow Journal of Innovative Optical Health Sciences Laser diffractometry ektacytometry shear flow diffraction pattern deformability of erythrocytes erythrocytes variance in shapes image analysis |
| title | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| title_full | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| title_fullStr | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| title_full_unstemmed | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| title_short | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| title_sort | study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow |
| topic | Laser diffractometry ektacytometry shear flow diffraction pattern deformability of erythrocytes erythrocytes variance in shapes image analysis |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S1793545815500315 |
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