Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage
The previous study successfully established an expression strain of ζ-carotene-like compounds (CLC) and demonstrated its remarkable antioxidant activity, which exhibited resistance to photodamage caused by UVB radiation on the skin following gavage administration. The objective of this study was to...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13970 |
| _version_ | 1797579717038047232 |
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| author | Liping Zhang Shaoxin Liang Zhi Zhang Kai Wang Junhan Cao Mengke Yao Ling Qin Changfeng Qu Jinlai Miao |
| author_facet | Liping Zhang Shaoxin Liang Zhi Zhang Kai Wang Junhan Cao Mengke Yao Ling Qin Changfeng Qu Jinlai Miao |
| author_sort | Liping Zhang |
| collection | DOAJ |
| description | The previous study successfully established an expression strain of ζ-carotene-like compounds (CLC) and demonstrated its remarkable antioxidant activity, which exhibited resistance to photodamage caused by UVB radiation on the skin following gavage administration. The objective of this study was to investigate the impact and mechanism of CLC on UVB-induced skin damage through topical application. Cell viability, anti-apoptotic activity, ROS scavenging ability, the inhibition of melanin synthesis, the regulation of inflammatory factors and collagen deposition were assessed in cells and mice using qRT-PCR, WB, Elisa assays, immunohistochemistry staining and biochemical kits, etc. The experimental results demonstrated that CLC-mitigated apoptosis induced by UVB irradiation up-regulated the Keap1/Nrf2/ARE antioxidant pathway to attenuate levels of ROS and inflammatory factors (NF-κB, TNF-α, IL-6 and IL-β), and suppressed MAPK/AP-1 and CAMP/PKA/CREB signaling pathways to mitigate collagen degradation, skin aging and melanin formation. In conclusion, this study underscored the potential of CLC as a safe and efficacious source of antioxidants, positioning it as a promising ingredient in the formulation of cosmetics targeting anti-aging, skin brightening and sunburn repair. |
| first_indexed | 2024-03-10T22:40:07Z |
| format | Article |
| id | doaj.art-349172d983024f65b6c533674a42d622 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:40:07Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-349172d983024f65b6c533674a42d6222023-11-19T11:05:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181397010.3390/ijms241813970Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin DamageLiping Zhang0Shaoxin Liang1Zhi Zhang2Kai Wang3Junhan Cao4Mengke Yao5Ling Qin6Changfeng Qu7Jinlai Miao8Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao 266071, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaDepartment of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao 266071, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaKey Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, ChinaThe previous study successfully established an expression strain of ζ-carotene-like compounds (CLC) and demonstrated its remarkable antioxidant activity, which exhibited resistance to photodamage caused by UVB radiation on the skin following gavage administration. The objective of this study was to investigate the impact and mechanism of CLC on UVB-induced skin damage through topical application. Cell viability, anti-apoptotic activity, ROS scavenging ability, the inhibition of melanin synthesis, the regulation of inflammatory factors and collagen deposition were assessed in cells and mice using qRT-PCR, WB, Elisa assays, immunohistochemistry staining and biochemical kits, etc. The experimental results demonstrated that CLC-mitigated apoptosis induced by UVB irradiation up-regulated the Keap1/Nrf2/ARE antioxidant pathway to attenuate levels of ROS and inflammatory factors (NF-κB, TNF-α, IL-6 and IL-β), and suppressed MAPK/AP-1 and CAMP/PKA/CREB signaling pathways to mitigate collagen degradation, skin aging and melanin formation. In conclusion, this study underscored the potential of CLC as a safe and efficacious source of antioxidants, positioning it as a promising ingredient in the formulation of cosmetics targeting anti-aging, skin brightening and sunburn repair.https://www.mdpi.com/1422-0067/24/18/13970ζ-carotene-like compoundsUVBskinantioxidantROS |
| spellingShingle | Liping Zhang Shaoxin Liang Zhi Zhang Kai Wang Junhan Cao Mengke Yao Ling Qin Changfeng Qu Jinlai Miao Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage International Journal of Molecular Sciences ζ-carotene-like compounds UVB skin antioxidant ROS |
| title | Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage |
| title_full | Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage |
| title_fullStr | Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage |
| title_full_unstemmed | Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage |
| title_short | Protective Effects of ζ-Carotene-like Compounds against Acute UVB-Induced Skin Damage |
| title_sort | protective effects of ζ carotene like compounds against acute uvb induced skin damage |
| topic | ζ-carotene-like compounds UVB skin antioxidant ROS |
| url | https://www.mdpi.com/1422-0067/24/18/13970 |
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