Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum
Bacteriophages have been investigated for clinical utility, both as diagnostic tools and as therapeutic interventions. In order to be applied successfully, a detailed understanding of the influence of the human matrix on the interaction between bacteriophage and the host bacterium is required. In th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/8/1748 |
| _version_ | 1797407422960107520 |
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| author | Matthew Brown Alex Hall Henriett Zahn Marcia Eisenberg Stephen Erickson |
| author_facet | Matthew Brown Alex Hall Henriett Zahn Marcia Eisenberg Stephen Erickson |
| author_sort | Matthew Brown |
| collection | DOAJ |
| description | Bacteriophages have been investigated for clinical utility, both as diagnostic tools and as therapeutic interventions. In order to be applied successfully, a detailed understanding of the influence of the human matrix on the interaction between bacteriophage and the host bacterium is required. In this study, a cocktail of luciferase bacteriophage reporters was assessed for functionality in a matrix containing human serum and spiked with <i>Staphylococcus aureus</i>. The inhibition of signal and loss of sensitivity was evident with minimal amounts of serum. This phenotype was independent of bacterial growth and bacteriophage viability. Serum-mediated loss of signal was common, albeit not universal, among <i>S. aureus</i> strains. Immunoglobulin G was identified as an inhibitory component and partial inhibition was observed with both the f(ab’)<sub>2</sub> and Fc region. A modified bacteriophage cocktail containing recombinant protein A was developed, which substantially improved signal without the need for additional sample purification. This study highlights the importance of assessing bacteriophage activity in relevant host matrices. Furthermore, it identifies an effective solution, recombinant protein A, for promoting bacteriophage-based detection of <i>S. aureus</i> in matrices containing human serum. |
| first_indexed | 2024-03-09T03:41:16Z |
| format | Article |
| id | doaj.art-34935e88d560416dbc7cf845585cd835 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:41:16Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-34935e88d560416dbc7cf845585cd8352023-12-03T14:39:26ZengMDPI AGViruses1999-49152022-08-01148174810.3390/v14081748Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human SerumMatthew Brown0Alex Hall1Henriett Zahn2Marcia Eisenberg3Stephen Erickson4Laboratory Corporation of America Holdings, Burlington, NC 27215, USALaboratory Corporation of America Holdings, Burlington, NC 27215, USALaboratory Corporation of America Holdings, New Brighton, MN 55112, USALaboratory Corporation of America Holdings, Burlington, NC 27215, USALaboratory Corporation of America Holdings, New Brighton, MN 55112, USABacteriophages have been investigated for clinical utility, both as diagnostic tools and as therapeutic interventions. In order to be applied successfully, a detailed understanding of the influence of the human matrix on the interaction between bacteriophage and the host bacterium is required. In this study, a cocktail of luciferase bacteriophage reporters was assessed for functionality in a matrix containing human serum and spiked with <i>Staphylococcus aureus</i>. The inhibition of signal and loss of sensitivity was evident with minimal amounts of serum. This phenotype was independent of bacterial growth and bacteriophage viability. Serum-mediated loss of signal was common, albeit not universal, among <i>S. aureus</i> strains. Immunoglobulin G was identified as an inhibitory component and partial inhibition was observed with both the f(ab’)<sub>2</sub> and Fc region. A modified bacteriophage cocktail containing recombinant protein A was developed, which substantially improved signal without the need for additional sample purification. This study highlights the importance of assessing bacteriophage activity in relevant host matrices. Furthermore, it identifies an effective solution, recombinant protein A, for promoting bacteriophage-based detection of <i>S. aureus</i> in matrices containing human serum.https://www.mdpi.com/1999-4915/14/8/1748phage-based detection<i>Staphylococcus aureus</i>bacteriophageluciferase reporter phagephage therapyserum |
| spellingShingle | Matthew Brown Alex Hall Henriett Zahn Marcia Eisenberg Stephen Erickson Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum Viruses phage-based detection <i>Staphylococcus aureus</i> bacteriophage luciferase reporter phage phage therapy serum |
| title | Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum |
| title_full | Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum |
| title_fullStr | Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum |
| title_full_unstemmed | Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum |
| title_short | Bacteriophage-Based Detection of <i>Staphylococcus aureus</i> in Human Serum |
| title_sort | bacteriophage based detection of i staphylococcus aureus i in human serum |
| topic | phage-based detection <i>Staphylococcus aureus</i> bacteriophage luciferase reporter phage phage therapy serum |
| url | https://www.mdpi.com/1999-4915/14/8/1748 |
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