Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone
Objective: The nucleus pulposus (NP) comprises notochordal NP cells (NCs) and chondrocyte-like NP cells (CLCs). Although morphological similarities between CLCs and chondrocytes have been reported, interactions between CLCs and NCs remain unclear. In this study, we aimed to clarify regulatory mechan...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1151947/full |
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| author | Hiroki Hagizawa Hiroki Hagizawa Saeko Koyamatsu Seiji Okada Takashi Kaito Noriyuki Tsumaki Noriyuki Tsumaki Noriyuki Tsumaki |
| author_facet | Hiroki Hagizawa Hiroki Hagizawa Saeko Koyamatsu Seiji Okada Takashi Kaito Noriyuki Tsumaki Noriyuki Tsumaki Noriyuki Tsumaki |
| author_sort | Hiroki Hagizawa |
| collection | DOAJ |
| description | Objective: The nucleus pulposus (NP) comprises notochordal NP cells (NCs) and chondrocyte-like NP cells (CLCs). Although morphological similarities between CLCs and chondrocytes have been reported, interactions between CLCs and NCs remain unclear. In this study, we aimed to clarify regulatory mechanisms of cells in the NP and chondrocytes.Design: We performed single-cell RNA sequencing (scRNA-seq) analysis of the articular cartilage (AC) and NP of three-year-old cynomolgus monkeys in which NCs were present. We then performed immunohistochemical analysis of NP and distal femur. We added sonic hedgehog (SHH) to primary chondrocyte culture.Results: The scRNA-seq analysis revealed that CLCs and some articular chondrocytes had similar gene expression profiles, particularly related to GLI1, the nuclear mediator of the hedgehog pathway. In the NP, cell–cell interaction analysis revealed SHH expression in NCs, resulting in hedgehog signaling to CLCs. In contrast, no hedgehog ligands were expressed by chondrocytes in AC samples. Immunohistochemical analysis of the distal end of femur indicated that SHH and Indian hedgehog (IHH) were expressed around the subchondral bone that was excluded from our scRNA-seq sample. scRNA-seq data analysis and treatment of primary chondrocytes with SHH revealed that hedgehog proteins mediated an increase in hypoxia-inducible factor 1-alpha (HIF-1α) levels.Conclusion: CLCs and some articular chondrocytes have similar transcriptional profiles, regulated by paracrine hedgehog proteins secreted from NCs in the NP and from the subchondral bone in the AC to promote the HIF-1α pathway. |
| first_indexed | 2024-04-09T12:38:26Z |
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| id | doaj.art-3494d11b51fa43959c722a6a34a18503 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-09T12:38:26Z |
| publishDate | 2023-05-01 |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-3494d11b51fa43959c722a6a34a185032023-05-15T04:57:53ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-05-011110.3389/fcell.2023.11519471151947Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral boneHiroki Hagizawa0Hiroki Hagizawa1Saeko Koyamatsu2Seiji Okada3Takashi Kaito4Noriyuki Tsumaki5Noriyuki Tsumaki6Noriyuki Tsumaki7Department of Tissue Biochemistry, Graduate School of Medicine and Frontier Biosciences, Osaka University, Osaka, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Osaka, JapanDepartment of Tissue Biochemistry, Graduate School of Medicine and Frontier Biosciences, Osaka University, Osaka, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Osaka, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Osaka, JapanDepartment of Tissue Biochemistry, Graduate School of Medicine and Frontier Biosciences, Osaka University, Osaka, JapanDepartment of Clinical Application, Center for IPS Cell Research and Application, Kyoto University, Kyoto, JapanPremium Research Institute for Human Metaverse Medicine (WPI-PRIMe), Osaka University, Osaka, JapanObjective: The nucleus pulposus (NP) comprises notochordal NP cells (NCs) and chondrocyte-like NP cells (CLCs). Although morphological similarities between CLCs and chondrocytes have been reported, interactions between CLCs and NCs remain unclear. In this study, we aimed to clarify regulatory mechanisms of cells in the NP and chondrocytes.Design: We performed single-cell RNA sequencing (scRNA-seq) analysis of the articular cartilage (AC) and NP of three-year-old cynomolgus monkeys in which NCs were present. We then performed immunohistochemical analysis of NP and distal femur. We added sonic hedgehog (SHH) to primary chondrocyte culture.Results: The scRNA-seq analysis revealed that CLCs and some articular chondrocytes had similar gene expression profiles, particularly related to GLI1, the nuclear mediator of the hedgehog pathway. In the NP, cell–cell interaction analysis revealed SHH expression in NCs, resulting in hedgehog signaling to CLCs. In contrast, no hedgehog ligands were expressed by chondrocytes in AC samples. Immunohistochemical analysis of the distal end of femur indicated that SHH and Indian hedgehog (IHH) were expressed around the subchondral bone that was excluded from our scRNA-seq sample. scRNA-seq data analysis and treatment of primary chondrocytes with SHH revealed that hedgehog proteins mediated an increase in hypoxia-inducible factor 1-alpha (HIF-1α) levels.Conclusion: CLCs and some articular chondrocytes have similar transcriptional profiles, regulated by paracrine hedgehog proteins secreted from NCs in the NP and from the subchondral bone in the AC to promote the HIF-1α pathway.https://www.frontiersin.org/articles/10.3389/fcell.2023.1151947/fullnucleus pulposus cellschondrocytessingle-cell RNA sequencinghedgehogHIF-1α |
| spellingShingle | Hiroki Hagizawa Hiroki Hagizawa Saeko Koyamatsu Seiji Okada Takashi Kaito Noriyuki Tsumaki Noriyuki Tsumaki Noriyuki Tsumaki Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone Frontiers in Cell and Developmental Biology nucleus pulposus cells chondrocytes single-cell RNA sequencing hedgehog HIF-1α |
| title | Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone |
| title_full | Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone |
| title_fullStr | Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone |
| title_full_unstemmed | Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone |
| title_short | Chondrocyte-like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine-regulated by hedgehog from notochordal cells and subchondral bone |
| title_sort | chondrocyte like cells in nucleus pulposus and articular chondrocytes have similar transcriptomic profiles and are paracrine regulated by hedgehog from notochordal cells and subchondral bone |
| topic | nucleus pulposus cells chondrocytes single-cell RNA sequencing hedgehog HIF-1α |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1151947/full |
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