Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan.
<h4>Background</h4>Few studies on molecular epidemiology have studied people with newly diagnosed HIV infection and ART Failure Patients at the same time in rural China. With more serious HIV epidemic than in other provinces in China, Sichuan is an area suitable for this study.<h4>...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2024-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298324&type=printable |
| _version_ | 1797301375991808000 |
|---|---|
| author | Xia Zhong Dan Yuan Shuang Feng Fan Yang Liu Ling Su Shi Jiao He Shu Liang Yi Yang |
| author_facet | Xia Zhong Dan Yuan Shuang Feng Fan Yang Liu Ling Su Shi Jiao He Shu Liang Yi Yang |
| author_sort | Xia Zhong |
| collection | DOAJ |
| description | <h4>Background</h4>Few studies on molecular epidemiology have studied people with newly diagnosed HIV infection and ART Failure Patients at the same time in rural China. With more serious HIV epidemic than in other provinces in China, Sichuan is an area suitable for this study.<h4>Objective</h4>To analyze the characteristics of HIV-1 molecular networks and factors related to network entry among newly diagnosed HIV infection and ART Failure Patients in three county-level cities (A, B, C) in Sichuan Province, to provide scientific basis for accurate prevention and control.<h4>Methods</h4>Nested PCR amplification method was used to amplify HIV-1 pol gene region of 530 blood samples, Sequencer 4.9 was used to edit, clean and splice the gene sequence, Bioedit correction, Fastree 2.1.8 and Figtree 1.4.2 to construct evolutionary tree and determine genotype. HyPhy2.2.4 and Cytoscape 3.6.1 software were used to construct molecular network. Logistic regression analysis was applied.<h4>Results</h4>523(98.68%) pol sequences were obtained, and a total of 518 valid sequences with basic information came into the final analyses. A total of 6 genotypes were detected, namely CRF01_AE (320,61.78%), CRF07_BC (149,28.76%), B (30,5.79%), CRF08_BC (11, 2.12%), CRF55_01B (6, 1.16%) and C (2, 0.39%). 186 of 518(35.91%) sequences entered the network at a genetic distance of 0.8%, forming 42 propagation clusters. "High-risk transmitters"(connected with two and more) accounted for 21.62%. Logistic regression showed that≥50 years old (OR = 2.474) were more risky than 18-49 years old, CRF07_BC sub-type (OR = 0.174) were less risky than CRF01_AE sub-type, B sub-type (OR = 6.698) is higher risky than CRF01_AE sub-type, and District B (OR = 0.077) less risky than that of A city.<h4>Conclusion</h4>The sources of HIV infection in rural Sichuan are diversified and complicated. The prevention and control of HIV infection in Sichuan Province should focus on strengthening the long-term dynamic detection of elderly population, B strain sub-type, and in City A. |
| first_indexed | 2024-03-07T23:21:38Z |
| format | Article |
| id | doaj.art-349af49d820e4ada8b06e7a3fb2d5912 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-07T23:21:38Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-349af49d820e4ada8b06e7a3fb2d59122024-02-21T05:31:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01192e029832410.1371/journal.pone.0298324Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan.Xia ZhongDan YuanShuang Feng FanYang LiuLing SuShi Jiao HeShu LiangYi Yang<h4>Background</h4>Few studies on molecular epidemiology have studied people with newly diagnosed HIV infection and ART Failure Patients at the same time in rural China. With more serious HIV epidemic than in other provinces in China, Sichuan is an area suitable for this study.<h4>Objective</h4>To analyze the characteristics of HIV-1 molecular networks and factors related to network entry among newly diagnosed HIV infection and ART Failure Patients in three county-level cities (A, B, C) in Sichuan Province, to provide scientific basis for accurate prevention and control.<h4>Methods</h4>Nested PCR amplification method was used to amplify HIV-1 pol gene region of 530 blood samples, Sequencer 4.9 was used to edit, clean and splice the gene sequence, Bioedit correction, Fastree 2.1.8 and Figtree 1.4.2 to construct evolutionary tree and determine genotype. HyPhy2.2.4 and Cytoscape 3.6.1 software were used to construct molecular network. Logistic regression analysis was applied.<h4>Results</h4>523(98.68%) pol sequences were obtained, and a total of 518 valid sequences with basic information came into the final analyses. A total of 6 genotypes were detected, namely CRF01_AE (320,61.78%), CRF07_BC (149,28.76%), B (30,5.79%), CRF08_BC (11, 2.12%), CRF55_01B (6, 1.16%) and C (2, 0.39%). 186 of 518(35.91%) sequences entered the network at a genetic distance of 0.8%, forming 42 propagation clusters. "High-risk transmitters"(connected with two and more) accounted for 21.62%. Logistic regression showed that≥50 years old (OR = 2.474) were more risky than 18-49 years old, CRF07_BC sub-type (OR = 0.174) were less risky than CRF01_AE sub-type, B sub-type (OR = 6.698) is higher risky than CRF01_AE sub-type, and District B (OR = 0.077) less risky than that of A city.<h4>Conclusion</h4>The sources of HIV infection in rural Sichuan are diversified and complicated. The prevention and control of HIV infection in Sichuan Province should focus on strengthening the long-term dynamic detection of elderly population, B strain sub-type, and in City A.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298324&type=printable |
| spellingShingle | Xia Zhong Dan Yuan Shuang Feng Fan Yang Liu Ling Su Shi Jiao He Shu Liang Yi Yang Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. PLoS ONE |
| title | Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. |
| title_full | Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. |
| title_fullStr | Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. |
| title_full_unstemmed | Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. |
| title_short | Molecular network analysis of 308 newly diagnosed HIV infection and 210 ART failure patients from rural counties in Sichuan. |
| title_sort | molecular network analysis of 308 newly diagnosed hiv infection and 210 art failure patients from rural counties in sichuan |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298324&type=printable |
| work_keys_str_mv | AT xiazhong molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT danyuan molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT shuangfengfan molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT yangliu molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT lingsu molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT shijiaohe molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT shuliang molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan AT yiyang molecularnetworkanalysisof308newlydiagnosedhivinfectionand210artfailurepatientsfromruralcountiesinsichuan |