Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats
Background: Methamphetamine (mAMP) as a recreational drug has devastating effects on the central nervous system (CNS). Several studies have shown that mAMP has inhibitory effects on oogenesis and spermatogenesis, and causes impaired fertility. This study designed to investigate the effect of mAM Pad...
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| Format: | Article |
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Kerman University of Medical Sciences
2017-11-01
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| Series: | Addiction and Health |
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| Online Access: | https://ahj.kmu.ac.ir/article_84729_2cfbb6a429307dec13acc651b701ce5c.pdf |
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| author | Arezoo Saberi Gholamreza Sepehri Zohreh Safi Behzad Razavi Faranak Jahandari Kouros Divsalar Ehsan Salarkia |
| author_facet | Arezoo Saberi Gholamreza Sepehri Zohreh Safi Behzad Razavi Faranak Jahandari Kouros Divsalar Ehsan Salarkia |
| author_sort | Arezoo Saberi |
| collection | DOAJ |
| description | Background: Methamphetamine (mAMP) as a recreational drug has devastating effects on the central nervous system (CNS). Several studies have shown that mAMP has inhibitory effects on oogenesis and spermatogenesis, and causes impaired fertility. This study designed to investigate the effect of mAM Padministration on histological changes and spermatogenesis indices in the testis of adult male rats.Methods: In this experimental study, 50 male Wistar rats were randomly divided into control (received no treatment, n = 10), vehicle (received saline for 7 and 14 days, n = 20), and experimental group [received mAMP, 5 ml/kg, intraperitoneal (IP) for 7 and 14 days, n = 20]. Testicular tissue samples were stained by hematoxylin and eosin (H&E) technique. For histological study, we counted the number of spermatogonia, spermatocytes and Leydig cells. Spermatogenesis indices which include: tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI) and the mean seminiferous tubules diameter (MSTD) were studied. Data were analyzed by one-way ANOVA, using SPSS software. P < 0.05 was considered statistically significant.Findings: This study showed that mAMP caused a significant decrease in number of seminiferous tubules cells and spermatogenesis in treated group compared with the control group. Moreover, results showed a significant decrease in spermatogenesis indices including TDI, SI, RI, and MSTD in 14th day, compared to control group (P < 0.001).Conclusion: The data showed the adverse effects of mAMP administration (for 7 and 14 days) on testes structure and spermatogenesis indices in rat testis tissue. The underlying mechanism(s) needs further investigation. |
| first_indexed | 2024-03-11T23:51:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2008-4633 2008-8469 |
| language | English |
| last_indexed | 2024-03-11T23:51:55Z |
| publishDate | 2017-11-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Addiction and Health |
| spelling | doaj.art-349b3fcc140a40b68ee3f42ef17827f12023-09-19T06:50:18ZengKerman University of Medical SciencesAddiction and Health2008-46332008-84692017-11-019419920584729Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male RatsArezoo Saberi0Gholamreza Sepehri1Zohreh Safi2Behzad Razavi3Faranak Jahandari4Kouros Divsalar5Ehsan Salarkia6Neuroscience Research Center, Institute of Neuropharmacology AND Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranProfessor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranEndocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences Kerman, IranNeuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranPhysiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, IranNeuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranBackground: Methamphetamine (mAMP) as a recreational drug has devastating effects on the central nervous system (CNS). Several studies have shown that mAMP has inhibitory effects on oogenesis and spermatogenesis, and causes impaired fertility. This study designed to investigate the effect of mAM Padministration on histological changes and spermatogenesis indices in the testis of adult male rats.Methods: In this experimental study, 50 male Wistar rats were randomly divided into control (received no treatment, n = 10), vehicle (received saline for 7 and 14 days, n = 20), and experimental group [received mAMP, 5 ml/kg, intraperitoneal (IP) for 7 and 14 days, n = 20]. Testicular tissue samples were stained by hematoxylin and eosin (H&E) technique. For histological study, we counted the number of spermatogonia, spermatocytes and Leydig cells. Spermatogenesis indices which include: tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI) and the mean seminiferous tubules diameter (MSTD) were studied. Data were analyzed by one-way ANOVA, using SPSS software. P < 0.05 was considered statistically significant.Findings: This study showed that mAMP caused a significant decrease in number of seminiferous tubules cells and spermatogenesis in treated group compared with the control group. Moreover, results showed a significant decrease in spermatogenesis indices including TDI, SI, RI, and MSTD in 14th day, compared to control group (P < 0.001).Conclusion: The data showed the adverse effects of mAMP administration (for 7 and 14 days) on testes structure and spermatogenesis indices in rat testis tissue. The underlying mechanism(s) needs further investigation.https://ahj.kmu.ac.ir/article_84729_2cfbb6a429307dec13acc651b701ce5c.pdfmethamphetaminehistologyspermatogenesistestisrats |
| spellingShingle | Arezoo Saberi Gholamreza Sepehri Zohreh Safi Behzad Razavi Faranak Jahandari Kouros Divsalar Ehsan Salarkia Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats Addiction and Health methamphetamine histology spermatogenesis testis rats |
| title | Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats |
| title_full | Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats |
| title_fullStr | Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats |
| title_full_unstemmed | Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats |
| title_short | Effects of Methamphetamine on Testes Histopathology and Spermatogenesis Indices of Adult Male Rats |
| title_sort | effects of methamphetamine on testes histopathology and spermatogenesis indices of adult male rats |
| topic | methamphetamine histology spermatogenesis testis rats |
| url | https://ahj.kmu.ac.ir/article_84729_2cfbb6a429307dec13acc651b701ce5c.pdf |
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