Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide
Plant-derived polysaccharide’s conformation and chain structure play a key role in their various biological activities. <i>Lycium barbarum</i> L. leaves’ polysaccharide is well renowned for its health functions. However, its functional bioactivities are greatly hindered by its compact gl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/7/1404 |
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| author | Majida Al-Wraikat Yun Liu Limei Wu Zeshan Ali Jianke Li |
| author_facet | Majida Al-Wraikat Yun Liu Limei Wu Zeshan Ali Jianke Li |
| author_sort | Majida Al-Wraikat |
| collection | DOAJ |
| description | Plant-derived polysaccharide’s conformation and chain structure play a key role in their various biological activities. <i>Lycium barbarum</i> L. leaves’ polysaccharide is well renowned for its health functions. However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight. To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation. In this study, the ethanol extract containing crude <i>Lycium barbarum</i> L. leaves’ polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) to produce degraded <i>Lycium barbarum</i> L. leaves’ polysaccharide. To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography–mass spectrometry (GC–MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM). Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition. However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation. |
| first_indexed | 2024-03-09T11:29:20Z |
| format | Article |
| id | doaj.art-349d04c905074c918f43caf01bdd7fee |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T11:29:20Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-349d04c905074c918f43caf01bdd7fee2023-11-30T23:53:58ZengMDPI AGPolymers2073-43602022-03-01147140410.3390/polym14071404Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen PeroxideMajida Al-Wraikat0Yun Liu1Limei Wu2Zeshan Ali3Jianke Li4College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, ChinaPlant-derived polysaccharide’s conformation and chain structure play a key role in their various biological activities. <i>Lycium barbarum</i> L. leaves’ polysaccharide is well renowned for its health functions. However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight. To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation. In this study, the ethanol extract containing crude <i>Lycium barbarum</i> L. leaves’ polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) to produce degraded <i>Lycium barbarum</i> L. leaves’ polysaccharide. To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography–mass spectrometry (GC–MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM). Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition. However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.https://www.mdpi.com/2073-4360/14/7/1404<i>Lycium barbarum</i> L. leavespolysaccharidedegradationascorbic acidmicrostructure |
| spellingShingle | Majida Al-Wraikat Yun Liu Limei Wu Zeshan Ali Jianke Li Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide Polymers <i>Lycium barbarum</i> L. leaves polysaccharide degradation ascorbic acid microstructure |
| title | Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide |
| title_full | Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide |
| title_fullStr | Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide |
| title_full_unstemmed | Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide |
| title_short | Structural Characterization of Degraded <i>Lycium barbarum</i> L. Leaves’ Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide |
| title_sort | structural characterization of degraded i lycium barbarum i l leaves polysaccharide using ascorbic acid and hydrogen peroxide |
| topic | <i>Lycium barbarum</i> L. leaves polysaccharide degradation ascorbic acid microstructure |
| url | https://www.mdpi.com/2073-4360/14/7/1404 |
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