| Summary: | Objective To investigate the expression levels of serum interleukin-32 (IL-32), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in patients with nonalcoholic fatty liver disease (NAFLD) and their correlation with the severity of NAFLD. Methods A total of 87 patients with NAFLD who attended to The Affiliated Qingdao Central Hospital of Qingdao University were enrolled as observation group, and according to hepatic controlled attenuation parameter, the observation group was divided into mild group, moderate group, and severe group. A total of 23 individuals who underwent physical examination du-ring the same period of time were enrolled as control group. The data on related indices were collected from all subjects, including serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transpeptidase (γ-GT), and ELISA was used to measure the serum levels of IL-32, IL-6, and TNF-α. Results There was a significant difference in serum ALT level between the control/mild/moderate group and the severe group and between the moderate group and the control group (Z=-53.66-26.83,P<0.05). There was a significant difference in serum AST level between the control/moderate group and the severe group (Z=-38.92,-28.32,P<0.05). There was a significant difference in serum γ-GT level between the moderate/severe group and the control group (Z=29.54,-45.65,P<0.05). There were significant differences in the serum levels of IL-32, IL-6, and TNF-α between the control/mild/moderate group and the severe group (tLSD=3.44-6.71,P<0.05), and there were significant differences in the serum levels of IL-32 and TNF-α between the moderate group and the control group (tLSD=-3.44, 2.64,P<0.05). CAP was positively correlated with the serum levels of ALT, AST, γ-GT, IL-32, IL-6, and TNF-α in all subjects (r=0.51-0.66,P<0.05). Conclusion There are significant increases in the serum levels of IL-32, IL-6, and TNF-α in patients with NAFLD, which are positively correlated with the severity of NAFLD, and the expression levels of these indices have a certain clinical significance in reflecting the degree of hepatocyte injury and the severity of NAFLD.
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