Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus
Abstract As a significant type of traumatic brain injury (TBI), blast-induced traumatic brain injury (bTBI) frequently results in severe neurological and psychological impairments. Due to its unique mechanistic and clinical features, bTBI presents diagnostic and therapeutic challenges compared to ot...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-023-02552-x |
| _version_ | 1827635609402867712 |
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| author | Lingxuan Zhang Qiuyun Yang Ruixuan Yuan Manrui Li Meili Lv Lin Zhang Xiaoqi Xie Weibo Liang Xiameng Chen |
| author_facet | Lingxuan Zhang Qiuyun Yang Ruixuan Yuan Manrui Li Meili Lv Lin Zhang Xiaoqi Xie Weibo Liang Xiameng Chen |
| author_sort | Lingxuan Zhang |
| collection | DOAJ |
| description | Abstract As a significant type of traumatic brain injury (TBI), blast-induced traumatic brain injury (bTBI) frequently results in severe neurological and psychological impairments. Due to its unique mechanistic and clinical features, bTBI presents diagnostic and therapeutic challenges compared to other TBI forms. The hippocampus, an important site for secondary injury of bTBI, serves as a key niche for neural regeneration and repair post-injury, and is closely associated with the neurological outcomes of bTBI patients. Nonetheless, the pathophysiological alterations of hippocampus underpinning bTBI remain enigmatic, and a corresponding transcriptomic dataset for research reference is yet to be established. In this investigation, the single-nucleus RNA sequencing (snRNA-seq) technique was employed to sequence individual hippocampal nuclei of mice from bTBI and sham group. Upon stringent quality control, gene expression data from 17,278 nuclei were obtained, with the dataset’s reliability substantiated through various analytical methods. This dataset holds considerable potential for exploring secondary hippocampal injury and neurogenesis mechanisms following bTBI, with important reference value for the identification of specific diagnostic and therapeutic targets for bTBI. |
| first_indexed | 2024-03-09T15:30:00Z |
| format | Article |
| id | doaj.art-349fb94bc29e449ab7063f47f3edabda |
| institution | Directory Open Access Journal |
| issn | 2052-4463 |
| language | English |
| last_indexed | 2024-03-09T15:30:00Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
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| series | Scientific Data |
| spelling | doaj.art-349fb94bc29e449ab7063f47f3edabda2023-11-26T12:17:49ZengNature PortfolioScientific Data2052-44632023-09-0110111110.1038/s41597-023-02552-xSingle-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampusLingxuan Zhang0Qiuyun Yang1Ruixuan Yuan2Manrui Li3Meili Lv4Lin Zhang5Xiaoqi Xie6Weibo Liang7Xiameng Chen8West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityDepartment of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityDepartment of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityDepartment of Immunology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityWest China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityDepartment of Critical Care Medicine, Sichuan UniversityDepartment of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityDepartment of Forensic Pathology and Forensic Clinical Medicine, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan UniversityAbstract As a significant type of traumatic brain injury (TBI), blast-induced traumatic brain injury (bTBI) frequently results in severe neurological and psychological impairments. Due to its unique mechanistic and clinical features, bTBI presents diagnostic and therapeutic challenges compared to other TBI forms. The hippocampus, an important site for secondary injury of bTBI, serves as a key niche for neural regeneration and repair post-injury, and is closely associated with the neurological outcomes of bTBI patients. Nonetheless, the pathophysiological alterations of hippocampus underpinning bTBI remain enigmatic, and a corresponding transcriptomic dataset for research reference is yet to be established. In this investigation, the single-nucleus RNA sequencing (snRNA-seq) technique was employed to sequence individual hippocampal nuclei of mice from bTBI and sham group. Upon stringent quality control, gene expression data from 17,278 nuclei were obtained, with the dataset’s reliability substantiated through various analytical methods. This dataset holds considerable potential for exploring secondary hippocampal injury and neurogenesis mechanisms following bTBI, with important reference value for the identification of specific diagnostic and therapeutic targets for bTBI.https://doi.org/10.1038/s41597-023-02552-x |
| spellingShingle | Lingxuan Zhang Qiuyun Yang Ruixuan Yuan Manrui Li Meili Lv Lin Zhang Xiaoqi Xie Weibo Liang Xiameng Chen Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus Scientific Data |
| title | Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus |
| title_full | Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus |
| title_fullStr | Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus |
| title_full_unstemmed | Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus |
| title_short | Single-nucleus transcriptomic mapping of blast-induced traumatic brain injury in mice hippocampus |
| title_sort | single nucleus transcriptomic mapping of blast induced traumatic brain injury in mice hippocampus |
| url | https://doi.org/10.1038/s41597-023-02552-x |
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