Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah
Background: Background: Intraoral radiography use some lower LET (Linear Energy Transfer) and could penetrate submandibular salivary gland. Radiography have negative impact which is decrease catalase enzyme of human body. Brown algae (Sargassum sp.) has a flavonoid antioxidant, polysaccharides as Fu...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Muhammadiyah Surabaya
2017-07-01
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| Series: | Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya |
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| Online Access: | http://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/490 |
| _version_ | 1811307036497936384 |
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| author | sariano ferni |
| author_facet | sariano ferni |
| author_sort | sariano ferni |
| collection | DOAJ |
| description | Background: Background: Intraoral radiography use some lower LET (Linear Energy Transfer) and could penetrate submandibular salivary gland. Radiography have negative impact which is decrease catalase enzyme of human body. Brown algae (Sargassum sp.) has a flavonoid antioxidant, polysaccharides as Fucoidan and alginat (Na-alginat) can be used for immunomodulator, antioxidative and activation modulation of immune. Purpose: To knowing effectiveness of brown algae (Sargassum sp.) on activity catalase enzyme submandibular salivary gland Rattus Novergicus strain Wistar with irradiation low LET. Material and Methods: 28 samples of Rattus Novergicus strain Wistar, weight 200gr, age 2-3months, gender male, sample divide into 4 groups, K1 (control with brown algae dosage 0,018mg/kgbw) K2 (use brown algae and irradiation 4 times), K3 (use brown algae and irradiation 8 times), K4 (use brown algae and irradiation 14 times). Brown algae been given 7days before apply irradiotion on day 8, then did euthanasia and took submandibular salivary gland. After that did measurement activity of catalase enzyme and counted by spectrophotometer with 240 λ. Result: Data were analyze by Shapiro-wilk, One Way ANOVA and Bonferroni. The activity of catalase enzyme have increased; 0,2586 ± 0,1050 (K1), 0,2595 ± 0,0630 (K2), 0,3252 ± 0,1663 (K3), 0,3668 ± 0,0852 (K4) but theres no significant differences activity of catalase enzyme between one group to other group. Conclusion: Brown algae dosage 0,018mg/kgbw can’t increase activity of catalase enzyme on Rattus Novergicus strain Wistar. |
| first_indexed | 2024-04-13T08:57:22Z |
| format | Article |
| id | doaj.art-34a09309aaf246a6af2ac9bfb2b4aa9e |
| institution | Directory Open Access Journal |
| issn | 2541-2272 2548-9526 |
| language | Indonesian |
| last_indexed | 2024-04-13T08:57:22Z |
| publishDate | 2017-07-01 |
| publisher | Universitas Muhammadiyah Surabaya |
| record_format | Article |
| series | Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya |
| spelling | doaj.art-34a09309aaf246a6af2ac9bfb2b4aa9e2022-12-22T02:53:15ZindUniversitas Muhammadiyah SurabayaQanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya2541-22722548-95262017-07-01102524Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendahsariano ferni0Departement Radiologi Kedokteran Gigi Fakultas Kedokteran Gigi Universitas Hang TuahBackground: Background: Intraoral radiography use some lower LET (Linear Energy Transfer) and could penetrate submandibular salivary gland. Radiography have negative impact which is decrease catalase enzyme of human body. Brown algae (Sargassum sp.) has a flavonoid antioxidant, polysaccharides as Fucoidan and alginat (Na-alginat) can be used for immunomodulator, antioxidative and activation modulation of immune. Purpose: To knowing effectiveness of brown algae (Sargassum sp.) on activity catalase enzyme submandibular salivary gland Rattus Novergicus strain Wistar with irradiation low LET. Material and Methods: 28 samples of Rattus Novergicus strain Wistar, weight 200gr, age 2-3months, gender male, sample divide into 4 groups, K1 (control with brown algae dosage 0,018mg/kgbw) K2 (use brown algae and irradiation 4 times), K3 (use brown algae and irradiation 8 times), K4 (use brown algae and irradiation 14 times). Brown algae been given 7days before apply irradiotion on day 8, then did euthanasia and took submandibular salivary gland. After that did measurement activity of catalase enzyme and counted by spectrophotometer with 240 λ. Result: Data were analyze by Shapiro-wilk, One Way ANOVA and Bonferroni. The activity of catalase enzyme have increased; 0,2586 ± 0,1050 (K1), 0,2595 ± 0,0630 (K2), 0,3252 ± 0,1663 (K3), 0,3668 ± 0,0852 (K4) but theres no significant differences activity of catalase enzyme between one group to other group. Conclusion: Brown algae dosage 0,018mg/kgbw can’t increase activity of catalase enzyme on Rattus Novergicus strain Wistar.http://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/490Brown algae, catalase enzyme, irradiation, low LET |
| spellingShingle | sariano ferni Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya Brown algae, catalase enzyme, irradiation, low LET |
| title | Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah |
| title_full | Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah |
| title_fullStr | Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah |
| title_full_unstemmed | Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah |
| title_short | Pengaruh Pemberian Alga Coklat (Sargassum sp.) Terhadap Enzim Katalase Kelenjar Submandibularis Tikus Rattus Norvegicus Strain Wistar Akibat Iradiasi Linear Energy Transfer (LET) Rendah |
| title_sort | pengaruh pemberian alga coklat sargassum sp terhadap enzim katalase kelenjar submandibularis tikus rattus norvegicus strain wistar akibat iradiasi linear energy transfer let rendah |
| topic | Brown algae, catalase enzyme, irradiation, low LET |
| url | http://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/490 |
| work_keys_str_mv | AT sarianoferni pengaruhpemberianalgacoklatsargassumspterhadapenzimkatalasekelenjarsubmandibularistikusrattusnorvegicusstrainwistarakibatiradiasilinearenergytransferletrendah |