Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine
Abstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a b...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02081-y |
| _version_ | 1818208739866443776 |
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| author | Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang |
| author_facet | Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang |
| author_sort | Na Wu |
| collection | DOAJ |
| description | Abstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae. |
| first_indexed | 2024-12-12T04:49:37Z |
| format | Article |
| id | doaj.art-34af404bdbc84d27bd5951a727deffad |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T04:49:37Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-34af404bdbc84d27bd5951a727deffad2022-12-22T00:37:30ZengBMCBiotechnology for Biofuels1754-68342021-12-0114111110.1186/s13068-021-02081-yTranscriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamineNa Wu0Jiahui Zhang1Wen Ou2Yaru Chen3Ru Wang4Ke Li5Xiao-man Sun6Yingfeng Li7Qing Xu8He Huang9School of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversitySchool of Food Science and Pharmaceutical Engineering, Nanjing Normal UniversityAbstract Rhizopus oryzae (R. oryzae) can effectively produce organic acids, and its pellet formation in seed cultures has been shown to significantly enhance subsequent fermentation processes. Despite advances in strain development, simple and effective methods for inducing pellet morphology and a basic understanding of the mechanisms controlling this process could facilitate substantial increases in efficiency and product output. Here, we report that 1.5% triethanolamine (TEOA) in seed culture medium can activate the growth of R. oryzae spores in compact and uniform pellets which is optimal for fermentation conditions. Analysis of fermentation kinetics showed that the production of fumaric and L-malic acid increases 293% and 177%, respectively. Transcriptomic analysis revealed that exposure of R. oryzae to 1.5% TEOA during the seed culture activated the phosphatidylinositol and mitogen-activated protein kinase signaling pathways. Theses pathways subsequently stimulated the downstream carbohydrate-active synthases and hydrolases that required for cell wall component synthesis and reconstruction. Our results thus provide insight into the regulatory pathways controlling pellet morphology germane to the viability of seed cultures, and provide valuable reference data for subsequent optimization of organic acid fermentation by R. oryzae.https://doi.org/10.1186/s13068-021-02081-yR. oryzaeSeed pellet formationTriethanolamineOrganic acidTranscriptomic analysis |
| spellingShingle | Na Wu Jiahui Zhang Wen Ou Yaru Chen Ru Wang Ke Li Xiao-man Sun Yingfeng Li Qing Xu He Huang Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine Biotechnology for Biofuels R. oryzae Seed pellet formation Triethanolamine Organic acid Transcriptomic analysis |
| title | Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine |
| title_full | Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine |
| title_fullStr | Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine |
| title_full_unstemmed | Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine |
| title_short | Transcriptome analysis of Rhizopus oryzae seed pellet formation using triethanolamine |
| title_sort | transcriptome analysis of rhizopus oryzae seed pellet formation using triethanolamine |
| topic | R. oryzae Seed pellet formation Triethanolamine Organic acid Transcriptomic analysis |
| url | https://doi.org/10.1186/s13068-021-02081-y |
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