PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella
A tea made with the leaves or stem bark of Bauhinia pulchella Benth (Fabaceae) is used in the treatment of diabetes. Ethanol (EEF) and aqueous (EAF) extracts were obtained from the leaves and the antioxidant and citotoxic activities were tested, as well as quantify the content of flavonoids and phen...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
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| Series: | Química Nova |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000400405&lng=en&tlng=en |
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| author | Adonias Almeida Carvalho Lucivania Rodrigues dos Santos Ruth Raquel Soares de Farias Mariana Helena Chaves Chistiane Mendes Feitosa Gerardo Magela Vieira Júnior Mônica Regina Silva de Araújo Paulo Michel Pinheiro Ferreira Claudia do Ó Pessoa |
| author_facet | Adonias Almeida Carvalho Lucivania Rodrigues dos Santos Ruth Raquel Soares de Farias Mariana Helena Chaves Chistiane Mendes Feitosa Gerardo Magela Vieira Júnior Mônica Regina Silva de Araújo Paulo Michel Pinheiro Ferreira Claudia do Ó Pessoa |
| author_sort | Adonias Almeida Carvalho |
| collection | DOAJ |
| description | A tea made with the leaves or stem bark of Bauhinia pulchella Benth (Fabaceae) is used in the treatment of diabetes. Ethanol (EEF) and aqueous (EAF) extracts were obtained from the leaves and the antioxidant and citotoxic activities were tested, as well as quantify the content of flavonoids and phenolic compounds (TPC). EEF and EAF showed similar profiles by HPLC, with the presence of three compounds. Column chromatography reverse phase (C18) and Sephadex LH-20, followed by semi preparative HPLC-C18 of EAF resulted in three flavonoids. Their structures were identified by 1H and 13C NMR as myricitrin (1), quercitrin (2) and afzelin (3). In assay of determination of reactive substances to thiobarbituric acid (TBARS), with DPPH, ABTS and nitric oxide (NO•) free radicals, EAF showed antioxidant potential higher than the EEF. This is the first report of the occurrence of the flavonoids 1-3, in the species B. pulchella. EEF and EAF were inactive in the cytotoxicity assays. In short, the polar extracts from the leaves of B. pulchella proved to be promising sources of biomolecules phenolic, with antioxidant potential, which may, in the future, be used as chemical markers for species and validation of therapeutic use. |
| first_indexed | 2024-12-12T06:44:12Z |
| format | Article |
| id | doaj.art-34b510ff33254fd091848b702b9e7e65 |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-12-12T06:44:12Z |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-34b510ff33254fd091848b702b9e7e652022-12-22T00:34:14ZengSociedade Brasileira de QuímicaQuímica Nova1678-706441440541110.21577/0100-4042.20170183S0100-40422018000400405PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchellaAdonias Almeida CarvalhoLucivania Rodrigues dos SantosRuth Raquel Soares de FariasMariana Helena ChavesChistiane Mendes FeitosaGerardo Magela Vieira JúniorMônica Regina Silva de AraújoPaulo Michel Pinheiro FerreiraClaudia do Ó PessoaA tea made with the leaves or stem bark of Bauhinia pulchella Benth (Fabaceae) is used in the treatment of diabetes. Ethanol (EEF) and aqueous (EAF) extracts were obtained from the leaves and the antioxidant and citotoxic activities were tested, as well as quantify the content of flavonoids and phenolic compounds (TPC). EEF and EAF showed similar profiles by HPLC, with the presence of three compounds. Column chromatography reverse phase (C18) and Sephadex LH-20, followed by semi preparative HPLC-C18 of EAF resulted in three flavonoids. Their structures were identified by 1H and 13C NMR as myricitrin (1), quercitrin (2) and afzelin (3). In assay of determination of reactive substances to thiobarbituric acid (TBARS), with DPPH, ABTS and nitric oxide (NO•) free radicals, EAF showed antioxidant potential higher than the EEF. This is the first report of the occurrence of the flavonoids 1-3, in the species B. pulchella. EEF and EAF were inactive in the cytotoxicity assays. In short, the polar extracts from the leaves of B. pulchella proved to be promising sources of biomolecules phenolic, with antioxidant potential, which may, in the future, be used as chemical markers for species and validation of therapeutic use.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000400405&lng=en&tlng=enantiradical activityantioxidantsflavonoidstotal phenolic |
| spellingShingle | Adonias Almeida Carvalho Lucivania Rodrigues dos Santos Ruth Raquel Soares de Farias Mariana Helena Chaves Chistiane Mendes Feitosa Gerardo Magela Vieira Júnior Mônica Regina Silva de Araújo Paulo Michel Pinheiro Ferreira Claudia do Ó Pessoa PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella Química Nova antiradical activity antioxidants flavonoids total phenolic |
| title | PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella |
| title_full | PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella |
| title_fullStr | PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella |
| title_full_unstemmed | PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella |
| title_short | PHENOLIC DERIVATIVES AND ANTIOXIDANT ACTIVITY OF POLAR EXTRACTS FROM Bauhinia pulchella |
| title_sort | phenolic derivatives and antioxidant activity of polar extracts from bauhinia pulchella |
| topic | antiradical activity antioxidants flavonoids total phenolic |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000400405&lng=en&tlng=en |
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