Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins
The outermost component of cell envelopes of most bacteria and almost all archaea comprise a protein lattice, which is termed Surface (S-)layer. The S-layer lattice constitutes a highly porous structure with regularly arranged pores in the nm-range. Some archaea thrive in extreme milieus, thus produ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/14/2502 |
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| author | Kevin Pfeifer Eva-Kathrin Ehmoser Simon K.-M. R. Rittmann Christa Schleper Dietmar Pum Uwe B. Sleytr Bernhard Schuster |
| author_facet | Kevin Pfeifer Eva-Kathrin Ehmoser Simon K.-M. R. Rittmann Christa Schleper Dietmar Pum Uwe B. Sleytr Bernhard Schuster |
| author_sort | Kevin Pfeifer |
| collection | DOAJ |
| description | The outermost component of cell envelopes of most bacteria and almost all archaea comprise a protein lattice, which is termed Surface (S-)layer. The S-layer lattice constitutes a highly porous structure with regularly arranged pores in the nm-range. Some archaea thrive in extreme milieus, thus producing highly stable S-layer protein lattices that aid in protecting the organisms. In the present study, fragments of the cell envelope from the hyperthermophilic acidophilic archaeon <i>Saccharolobus solfataricus</i> P2 (SSO) have been isolated by two different methods and characterized. The organization of the fragments and the molecular sieving properties have been elucidated by transmission electron microscopy and by determining the retention efficiency of proteins varying in size, respectively. The porosity of the archaeal S-layer fragments was determined to be 45%. S-layer fragments of SSO showed a retention efficiency of up to 100% for proteins having a molecular mass of ≥ 66 kDa. Moreover, the extraction costs for SSO fragments have been reduced by more than 80% compared to conventional methods, which makes the use of these archaeal S-layer material economically attractive. |
| first_indexed | 2024-03-09T13:15:18Z |
| format | Article |
| id | doaj.art-34b7c19d796045dbbc9407b45774d890 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T13:15:18Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-34b7c19d796045dbbc9407b45774d8902023-11-30T21:36:37ZengMDPI AGNanomaterials2079-49912022-07-011214250210.3390/nano12142502Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer ProteinsKevin Pfeifer0Eva-Kathrin Ehmoser1Simon K.-M. R. Rittmann2Christa Schleper3Dietmar Pum4Uwe B. Sleytr5Bernhard Schuster6Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences, 1190 Vienna, AustriaInstitute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences, 1190 Vienna, AustriaArchaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, AustriaArchaea Biology and Ecogenomics Division, Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, AustriaInstitute of Biophysics, University of Natural Resources and Life Sciences, 1190 Vienna, AustriaInstitute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences, 1190 Vienna, AustriaInstitute of Synthetic Bioarchitectures, University of Natural Resources and Life Sciences, 1190 Vienna, AustriaThe outermost component of cell envelopes of most bacteria and almost all archaea comprise a protein lattice, which is termed Surface (S-)layer. The S-layer lattice constitutes a highly porous structure with regularly arranged pores in the nm-range. Some archaea thrive in extreme milieus, thus producing highly stable S-layer protein lattices that aid in protecting the organisms. In the present study, fragments of the cell envelope from the hyperthermophilic acidophilic archaeon <i>Saccharolobus solfataricus</i> P2 (SSO) have been isolated by two different methods and characterized. The organization of the fragments and the molecular sieving properties have been elucidated by transmission electron microscopy and by determining the retention efficiency of proteins varying in size, respectively. The porosity of the archaeal S-layer fragments was determined to be 45%. S-layer fragments of SSO showed a retention efficiency of up to 100% for proteins having a molecular mass of ≥ 66 kDa. Moreover, the extraction costs for SSO fragments have been reduced by more than 80% compared to conventional methods, which makes the use of these archaeal S-layer material economically attractive.https://www.mdpi.com/2079-4991/12/14/2502archaeaS-layer proteinArchaea Biotechnologymolecular sievingextraction methodbiomimetics |
| spellingShingle | Kevin Pfeifer Eva-Kathrin Ehmoser Simon K.-M. R. Rittmann Christa Schleper Dietmar Pum Uwe B. Sleytr Bernhard Schuster Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins Nanomaterials archaea S-layer protein Archaea Biotechnology molecular sieving extraction method biomimetics |
| title | Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins |
| title_full | Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins |
| title_fullStr | Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins |
| title_full_unstemmed | Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins |
| title_short | Isolation and Characterization of Cell Envelope Fragments Comprising Archaeal S-Layer Proteins |
| title_sort | isolation and characterization of cell envelope fragments comprising archaeal s layer proteins |
| topic | archaea S-layer protein Archaea Biotechnology molecular sieving extraction method biomimetics |
| url | https://www.mdpi.com/2079-4991/12/14/2502 |
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