| Summary: | Ischemia-reperfusion (IR) can lead to serious tissue oxidative injury in animals. ShuJinHuoXue tablet (SJHXT) is a Chinese Traditional Medicine which can relax the muscles and stimulate the blood circulation and has been used as a clinical medicine. In the present study, we investigated the effects of SJHXT pretreatment on oxidative injury using an animal model of acute limb IR. Results showed that SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced serum endothelin-1 (ET-1), thromboxane B2 (TXB<sub>2</sub>) levels and thromboxane B2/<em>6</em>-<em>keto</em>- prostaglandin F1α (TXB<sub>2</sub>/6-Keto-PGF<sub>1α</sub>), wet weight/dried weight (W/D) ratio, m<em>yeloperoxidase</em> (MPO), creatine kinase (CK), lactate dehydrogenase (LDH) activities, and increased serum nitric oxide (NO), 6-Keto-PGF<sub>1α</sub> levels and NO/ET-1 ratio in the IR+SJHXT groups. In addition, the SJHXT pre-treatment (200, 300 and 400 mg/kg/day) markedly reduced skeletal muscle Ca<sup>2+</sup>, malondialdehyde (MDA) levels, increased Na<sup>+</sup>-K<sup>+</sup>-ATPase, Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. Our results suggest that SJHXT pre-treatment may improve skeletal muscle blood vessel microcirculation, decrease skeletal muscle oxidative injury and enhance antioxidant enzymes activities in IR animals.
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