Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir

E.coli is natural flour in large intestine and main common pathogen between human and animal , and its transportable by food , that its important cause of diarrhea infections in cows , particularly in calves. The aim of this survey is identify and separating E.coli and verotoxicogenic, that by refer...

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Main Authors: M Khakpour, F Nazeri, J Khandagi, J Shaieg
Format: Article
Language:fas
Published: Islamic Azad University, Tabriz Branch 2012-02-01
Series:Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī
Subjects:
Online Access:http://jvcp.iaut.ac.ir/article_517939_287f5fdebafa1cc7786e556ab1ef5d11.pdf
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author M Khakpour
F Nazeri
J Khandagi
J Shaieg
author_facet M Khakpour
F Nazeri
J Khandagi
J Shaieg
author_sort M Khakpour
collection DOAJ
description E.coli is natural flour in large intestine and main common pathogen between human and animal , and its transportable by food , that its important cause of diarrhea infections in cows , particularly in calves. The aim of this survey is identify and separating E.coli and verotoxicogenic, that by referring to Tabriz slaughterhouse in chance 43 excrement samples from calves and 151 excrement samples from cows has been taken and transport to microbiology laboratory of veterinary faculty of Islamic azad TabrizUniversity. After in riching the samples and culturing in plates this results has been taken: From 194 taken samples, 113 E.coli samples were separated that were 85 positive sorbitol samples and 28 negative sorbitol samples .From 85 positive sorbitol samples were 19 samples of calves and 66 samples of cows and from 28 negative sorbitol samples were 13 samples of calves and 15 samples of cows. Also serological test accomplished for determining E.coli non O157 serotypes on the positive sorbitol samples that 9 samples of cows and 42 samples of cows demonstrate positive reaction, then on 28 negative sorbitol samples and 85 positive sorbitol samples, the PCR test accomplished by using secans of stx1, stx2 gens, that 15 samples of 28 negative sorbitol samples and 19 samples of 42 positive sorbitol samples were E.coli and verotoxicogenic. The result demonstrated that high amount of E.coli and verotoxicogenic exist in excrement of cows and calves slaughtered in Tabriz slaughterhouse.
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spelling doaj.art-34bd3576862e4c01a4a3ffeba37ef73e2022-12-21T18:15:10ZfasIslamic Azad University, Tabriz BranchĀsīb/shināsī-i Darmāngāhī-i Dāmpizishkī2322-47462476-69842012-02-0154 (20) زمستان13791386517939Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoirM Khakpour0F Nazeri1J Khandagi2J Shaieg3Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, IranGgraduate of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, IranDepartment of Food Industry, Sarab Branch, Islamic Azad University, Sarab, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, IranE.coli is natural flour in large intestine and main common pathogen between human and animal , and its transportable by food , that its important cause of diarrhea infections in cows , particularly in calves. The aim of this survey is identify and separating E.coli and verotoxicogenic, that by referring to Tabriz slaughterhouse in chance 43 excrement samples from calves and 151 excrement samples from cows has been taken and transport to microbiology laboratory of veterinary faculty of Islamic azad TabrizUniversity. After in riching the samples and culturing in plates this results has been taken: From 194 taken samples, 113 E.coli samples were separated that were 85 positive sorbitol samples and 28 negative sorbitol samples .From 85 positive sorbitol samples were 19 samples of calves and 66 samples of cows and from 28 negative sorbitol samples were 13 samples of calves and 15 samples of cows. Also serological test accomplished for determining E.coli non O157 serotypes on the positive sorbitol samples that 9 samples of cows and 42 samples of cows demonstrate positive reaction, then on 28 negative sorbitol samples and 85 positive sorbitol samples, the PCR test accomplished by using secans of stx1, stx2 gens, that 15 samples of 28 negative sorbitol samples and 19 samples of 42 positive sorbitol samples were E.coli and verotoxicogenic. The result demonstrated that high amount of E.coli and verotoxicogenic exist in excrement of cows and calves slaughtered in Tabriz slaughterhouse.http://jvcp.iaut.ac.ir/article_517939_287f5fdebafa1cc7786e556ab1ef5d11.pdfVerotoxicogenic E.coliCowCalveTabriz slaughterhousePCR
spellingShingle M Khakpour
F Nazeri
J Khandagi
J Shaieg
Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
Āsīb/shināsī-i Darmāngāhī-i Dāmpizishkī
Verotoxicogenic E.coli
Cow
Calve
Tabriz slaughterhouse
PCR
title Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
title_full Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
title_fullStr Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
title_full_unstemmed Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
title_short Prevalence of verotoxigenic Escherichia coli (VTEC) on slaughtered cattle and calves in Tabriz abattoir
title_sort prevalence of verotoxigenic escherichia coli vtec on slaughtered cattle and calves in tabriz abattoir
topic Verotoxicogenic E.coli
Cow
Calve
Tabriz slaughterhouse
PCR
url http://jvcp.iaut.ac.ir/article_517939_287f5fdebafa1cc7786e556ab1ef5d11.pdf
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AT jkhandagi prevalenceofverotoxigenicescherichiacolivteconslaughteredcattleandcalvesintabrizabattoir
AT jshaieg prevalenceofverotoxigenicescherichiacolivteconslaughteredcattleandcalvesintabrizabattoir