Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes
Abstract Plants have evolved sophisticated defense systems to enhance drought tolerance. These include the microRNA (miRNA) group of small noncoding RNAs that act as post‐transcriptional regulators; however, details of the mechanisms by which they confer drought tolerance are not well understood. He...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.374 |
| _version_ | 1818952587399921664 |
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| author | Taeyoung Um Joohee Choi Taehyeon Park Pil Joong Chung Se Eun Jung Jae Sung Shim Youn Shic Kim Ik‐Young Choi Soo Chul Park Se‐Jun Oh Jun Sung Seo Ju‐Kon Kim |
| author_facet | Taeyoung Um Joohee Choi Taehyeon Park Pil Joong Chung Se Eun Jung Jae Sung Shim Youn Shic Kim Ik‐Young Choi Soo Chul Park Se‐Jun Oh Jun Sung Seo Ju‐Kon Kim |
| author_sort | Taeyoung Um |
| collection | DOAJ |
| description | Abstract Plants have evolved sophisticated defense systems to enhance drought tolerance. These include the microRNA (miRNA) group of small noncoding RNAs that act as post‐transcriptional regulators; however, details of the mechanisms by which they confer drought tolerance are not well understood. Here, we show that osa‐MIR171f, a member of osa‐MIR171 gene family, is mainly expressed in response to drought stress and regulates the transcript levels of SCARECROW‐LIKE6‐I (SCL6‐I) and SCL6‐II in rice (Oryza sativa). The SCL6 genes are known to be involved in shoot branching and flag leaf morphology. Osa‐MIR171f‐overexpressing (osa‐MIR171f‐OE) transgenic plants showed reduced drought symptoms compared with non‐transgenic (NT) control plants under both field drought and polyethylene glycol (PEG)‐mediated dehydration stress conditions. Transcriptome analysis of osa‐MIR171f‐OE plants and osa‐mir171f‐knockout (K/O) lines generated by clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) revealed that osa‐mature‐miR171a‐f (osa‐miR171) regulates the expression of flavonoid biosynthesis genes, consequently leading to drought tolerance. This upregulation in the osa‐MIR171f‐OE plants, which did not occur in NT control plants, was observed under both normal and drought conditions. Our findings indicate that osa‐miR171 plays a role in drought tolerance by regulating SCL6‐I and SCL6‐II transcript levels. |
| first_indexed | 2024-12-20T09:52:45Z |
| format | Article |
| id | doaj.art-34bf8d81b98c43d0a420bdb8cb2773da |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-20T09:52:45Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-34bf8d81b98c43d0a420bdb8cb2773da2022-12-21T19:44:32ZengWileyPlant Direct2475-44552022-01-0161n/an/a10.1002/pld3.374Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genesTaeyoung Um0Joohee Choi1Taehyeon Park2Pil Joong Chung3Se Eun Jung4Jae Sung Shim5Youn Shic Kim6Ik‐Young Choi7Soo Chul Park8Se‐Jun Oh9Jun Sung Seo10Ju‐Kon Kim11Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaDepartment of Agricultural and life industry Kangwon National University Chuncheon South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaLaSemilla Co. Ltd Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaGraduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology Seoul National University Pyeongchang South KoreaAbstract Plants have evolved sophisticated defense systems to enhance drought tolerance. These include the microRNA (miRNA) group of small noncoding RNAs that act as post‐transcriptional regulators; however, details of the mechanisms by which they confer drought tolerance are not well understood. Here, we show that osa‐MIR171f, a member of osa‐MIR171 gene family, is mainly expressed in response to drought stress and regulates the transcript levels of SCARECROW‐LIKE6‐I (SCL6‐I) and SCL6‐II in rice (Oryza sativa). The SCL6 genes are known to be involved in shoot branching and flag leaf morphology. Osa‐MIR171f‐overexpressing (osa‐MIR171f‐OE) transgenic plants showed reduced drought symptoms compared with non‐transgenic (NT) control plants under both field drought and polyethylene glycol (PEG)‐mediated dehydration stress conditions. Transcriptome analysis of osa‐MIR171f‐OE plants and osa‐mir171f‐knockout (K/O) lines generated by clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) revealed that osa‐mature‐miR171a‐f (osa‐miR171) regulates the expression of flavonoid biosynthesis genes, consequently leading to drought tolerance. This upregulation in the osa‐MIR171f‐OE plants, which did not occur in NT control plants, was observed under both normal and drought conditions. Our findings indicate that osa‐miR171 plays a role in drought tolerance by regulating SCL6‐I and SCL6‐II transcript levels.https://doi.org/10.1002/pld3.374droughtflavonoidsmiR171Osa‐miR171fRiceSCL6 |
| spellingShingle | Taeyoung Um Joohee Choi Taehyeon Park Pil Joong Chung Se Eun Jung Jae Sung Shim Youn Shic Kim Ik‐Young Choi Soo Chul Park Se‐Jun Oh Jun Sung Seo Ju‐Kon Kim Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes Plant Direct drought flavonoids miR171 Osa‐miR171f Rice SCL6 |
| title | Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| title_full | Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| title_fullStr | Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| title_full_unstemmed | Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| title_short | Rice microRNA171f/SCL6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| title_sort | rice microrna171f scl6 module enhances drought tolerance by regulation of flavonoid biosynthesis genes |
| topic | drought flavonoids miR171 Osa‐miR171f Rice SCL6 |
| url | https://doi.org/10.1002/pld3.374 |
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