Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients
We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson’s disease (PD) patients’ peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients’ skin, using whole-geno...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2019.00013/full |
| _version_ | 1818493132732366848 |
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| author | Lille Kurvits Lille Kurvits Ene Reimann Liis Kadastik-Eerme Laura Truu Külli Kingo Külli Kingo Triin Erm Sulev Kõks Sulev Kõks Pille Taba Anu Planken |
| author_facet | Lille Kurvits Lille Kurvits Ene Reimann Liis Kadastik-Eerme Laura Truu Külli Kingo Külli Kingo Triin Erm Sulev Kõks Sulev Kõks Pille Taba Anu Planken |
| author_sort | Lille Kurvits |
| collection | DOAJ |
| description | We report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson’s disease (PD) patients’ peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients’ skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients’ serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients. |
| first_indexed | 2024-12-10T17:51:12Z |
| format | Article |
| id | doaj.art-34c10cc9c7524370a09e1faee0ab276e |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-10T17:51:12Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-34c10cc9c7524370a09e1faee0ab276e2022-12-22T01:39:03ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-01-011310.3389/fnins.2019.00013421012Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease PatientsLille Kurvits0Lille Kurvits1Ene Reimann2Liis Kadastik-Eerme3Laura Truu4Külli Kingo5Külli Kingo6Triin Erm7Sulev Kõks8Sulev Kõks9Pille Taba10Anu Planken11Department of Neurology and Neurosurgery, University of Tartu, Tartu, EstoniaDepartment of Neurology, Charité – Universitätsmedizin Berlin, Berlin, GermanyInstitute of Pathophysiology, University of Tartu, Tartu, EstoniaDepartment of Neurology and Neurosurgery, University of Tartu, Tartu, EstoniaLaboratory of Bioenergetics, National Institute of Chemical Physics and Biophysics, Tallinn, EstoniaDepartment of Dermatology, University of Tartu, Tartu, EstoniaDermatology Clinic, Tartu University Hospital, Tartu, EstoniaDepartment of Pathology, Tartu University Hospital, Tartu, EstoniaCentre for Comparative Genomics, Murdoch University, Perth, WA, AustraliaPerron Institute for Neurological and Translational Science, University of Western Australia, Perth, WA, AustraliaDepartment of Neurology and Neurosurgery, University of Tartu, Tartu, Estonia0Oncology and Haematology Clinic, North-Estonian Medical Centre, Tallinn, EstoniaWe report the changed levels of serum amyloid alpha, an immunologically active protein, in Parkinson’s disease (PD) patients’ peripheral tissues. We have previously shown that Saa-1 and -2 (serum amyloid alpha-1,-2, genes) were among the top downregulated genes in PD patients’ skin, using whole-genome RNA sequencing. In the current study, we characterized the gene and protein expression profiles of skin and blood samples from patients with confirmed PD diagnosis and age/sex matched controls. qRT-PCR analysis of PD skin demonstrated downregulation of Saa-1 and -2 genes in PD patients. However, the lowered amount of protein could not be visualized using immunohistochemistry, due to low quantity of SAA (Serum Amyloid Alpha, protein) in skin. Saa-1 and -2 expression levels in whole blood were below detection threshold based on RNA sequencing, however significantly lowered protein levels of SAA1/2 in PD patients’ serum were shown with ELISA, implying that SAA is secreted into the blood. These results show that SAA is differentially expressed in the peripheral tissues of PD patients.https://www.frontiersin.org/article/10.3389/fnins.2019.00013/fullneurodegenarative diseaseserum amyloid A (SAA)Parkinson’s disease (PD)intrinsically disordered protein (IDP)skin biopsy |
| spellingShingle | Lille Kurvits Lille Kurvits Ene Reimann Liis Kadastik-Eerme Laura Truu Külli Kingo Külli Kingo Triin Erm Sulev Kõks Sulev Kõks Pille Taba Anu Planken Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients Frontiers in Neuroscience neurodegenarative disease serum amyloid A (SAA) Parkinson’s disease (PD) intrinsically disordered protein (IDP) skin biopsy |
| title | Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients |
| title_full | Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients |
| title_fullStr | Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients |
| title_full_unstemmed | Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients |
| title_short | Serum Amyloid Alpha Is Downregulated in Peripheral Tissues of Parkinson’s Disease Patients |
| title_sort | serum amyloid alpha is downregulated in peripheral tissues of parkinson s disease patients |
| topic | neurodegenarative disease serum amyloid A (SAA) Parkinson’s disease (PD) intrinsically disordered protein (IDP) skin biopsy |
| url | https://www.frontiersin.org/article/10.3389/fnins.2019.00013/full |
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