Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris
Xanthomonas campestris pv. campestris (Xcc) is a vascular bacteria pathogen causing black rot in cabbage. Here, the resistance mechanisms of cabbage against Xcc infection were explored by integrated metabolome and transcriptome analysis. Pathogen perception, hormone metabolisms, sugar metabolisms, a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1005764/full |
| _version_ | 1828337665420820480 |
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| author | Qingguo Sun Zhongmin Xu Wei Huang Dawei Li Qi Zeng Lin Chen Baohua Li Enhui Zhang |
| author_facet | Qingguo Sun Zhongmin Xu Wei Huang Dawei Li Qi Zeng Lin Chen Baohua Li Enhui Zhang |
| author_sort | Qingguo Sun |
| collection | DOAJ |
| description | Xanthomonas campestris pv. campestris (Xcc) is a vascular bacteria pathogen causing black rot in cabbage. Here, the resistance mechanisms of cabbage against Xcc infection were explored by integrated metabolome and transcriptome analysis. Pathogen perception, hormone metabolisms, sugar metabolisms, and phenylpropanoid metabolisms in cabbage were systemically re-programmed at both transcriptional and metabolic levels after Xcc infection. Notably, the salicylic acid (SA) metabolism pathway was highly enriched in resistant lines following Xcc infection, indicating that the SA metabolism pathway may positively regulate the resistance of Xcc. Moreover, we also validated our hypothesis by showing that the flavonoid pathway metabolites chlorogenic acid and caffeic acid could effectively inhibit the growth of Xcc. These findings provide valuable insights and resource datasets for further exploring Xcc–cabbage interactions and help uncover molecular breeding targets for black rot-resistant varieties in cabbage. |
| first_indexed | 2024-04-13T22:18:07Z |
| format | Article |
| id | doaj.art-34c15a81a7314d79acd77d50d6c77356 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T22:18:07Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-34c15a81a7314d79acd77d50d6c773562022-12-22T02:27:23ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10057641005764Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestrisQingguo SunZhongmin XuWei HuangDawei LiQi ZengLin ChenBaohua LiEnhui ZhangXanthomonas campestris pv. campestris (Xcc) is a vascular bacteria pathogen causing black rot in cabbage. Here, the resistance mechanisms of cabbage against Xcc infection were explored by integrated metabolome and transcriptome analysis. Pathogen perception, hormone metabolisms, sugar metabolisms, and phenylpropanoid metabolisms in cabbage were systemically re-programmed at both transcriptional and metabolic levels after Xcc infection. Notably, the salicylic acid (SA) metabolism pathway was highly enriched in resistant lines following Xcc infection, indicating that the SA metabolism pathway may positively regulate the resistance of Xcc. Moreover, we also validated our hypothesis by showing that the flavonoid pathway metabolites chlorogenic acid and caffeic acid could effectively inhibit the growth of Xcc. These findings provide valuable insights and resource datasets for further exploring Xcc–cabbage interactions and help uncover molecular breeding targets for black rot-resistant varieties in cabbage.https://www.frontiersin.org/articles/10.3389/fpls.2022.1005764/fullcabbageblack rottranscriptomemetabolomeSA metabolismflavonoid |
| spellingShingle | Qingguo Sun Zhongmin Xu Wei Huang Dawei Li Qi Zeng Lin Chen Baohua Li Enhui Zhang Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris Frontiers in Plant Science cabbage black rot transcriptome metabolome SA metabolism flavonoid |
| title | Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris |
| title_full | Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris |
| title_fullStr | Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris |
| title_full_unstemmed | Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris |
| title_short | Integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways’ key roles in cabbage’s defense responses to Xanthomonas campestris pv. campestris |
| title_sort | integrated metabolome and transcriptome analysis reveals salicylic acid and flavonoid pathways key roles in cabbage s defense responses to xanthomonas campestris pv campestris |
| topic | cabbage black rot transcriptome metabolome SA metabolism flavonoid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1005764/full |
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