Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography
Acotiamide hydrochloride trihydrate, which is the first gastrointestinal prokinetic agent, has been proven effective in treating functional malnutrition. There is little method in analyzing this new medicine. The only reported method is using High Performance Liquid Chromatography (HPLC), with metha...
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| Format: | Article |
| Language: | English |
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Science Press, PR China
2014-11-01
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| Series: | Yankuang ceshi |
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| Online Access: | http://www.ykcs.ac.cn/cn/article/id/742ab818-c3d6-43ac-a7bd-8ee3244cc72b |
| _version_ | 1811168394510073856 |
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| author | ZHOU Wei-qun QIAO Jun-qin LIAN Hong-zhen |
| author_facet | ZHOU Wei-qun QIAO Jun-qin LIAN Hong-zhen |
| author_sort | ZHOU Wei-qun |
| collection | DOAJ |
| description | Acotiamide hydrochloride trihydrate, which is the first gastrointestinal prokinetic agent, has been proven effective in treating functional malnutrition. There is little method in analyzing this new medicine. The only reported method is using High Performance Liquid Chromatography (HPLC), with methanol-monopotassium phosphate-sodium octanesulfonate as the mobile phase. However, this method is time wasting and unstable because of the using of an ion paring agent in the mobile phase. In this paper, a new HPLC method is established to quantitatively analyze industrial acotiamide hydrochloride trihydrate based on its amphoteric structure. The separation is accomplished in a C18 (4.6 mm×150 mm, 5 μm) column with methanol and 20 mmol/L ammonium acetate aqueous solution (pH 6.8) (45:55, V/V) as the mobile phase. The detection wavelength is set at UV 280 nm. The retention time of acotiamide hydrochloride trihydrate is 8 min with good peak shape, and the total analysis time is 23 min. Under the optimized chromatographic condition, the calibration curve of acotiamide hydrochloride trihydrate is linear in the range of 0.0006 and 1.0 mg/mL, detection limit is 0.0002 mg/mL, and both the intra-and inter-day relative standard deviations (RSDs) are lower than 1%. This method contains no ion paring agent, benefiting the rapid balance of chromatographic condition and improving the analysis repeatability and maneuverability. The method can be performed for rapid detection of acotiamide hydrochloride trihydrate and used in the quality control of raw medicine. |
| first_indexed | 2024-04-10T16:25:34Z |
| format | Article |
| id | doaj.art-34c2337a7ffc4212b59680d07ab596c2 |
| institution | Directory Open Access Journal |
| issn | 0254-5357 |
| language | English |
| last_indexed | 2024-04-10T16:25:34Z |
| publishDate | 2014-11-01 |
| publisher | Science Press, PR China |
| record_format | Article |
| series | Yankuang ceshi |
| spelling | doaj.art-34c2337a7ffc4212b59680d07ab596c22023-02-09T06:28:41ZengScience Press, PR ChinaYankuang ceshi0254-53572014-11-01336871875ykcs-33-6-871Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid ChromatographyZHOU Wei-qun0QIAO Jun-qin1LIAN Hong-zhen2Nantong Radio and TV University, Nantong 226006, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210093, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Center of Materials Analysis, Nanjing University, Nanjing 210093, ChinaAcotiamide hydrochloride trihydrate, which is the first gastrointestinal prokinetic agent, has been proven effective in treating functional malnutrition. There is little method in analyzing this new medicine. The only reported method is using High Performance Liquid Chromatography (HPLC), with methanol-monopotassium phosphate-sodium octanesulfonate as the mobile phase. However, this method is time wasting and unstable because of the using of an ion paring agent in the mobile phase. In this paper, a new HPLC method is established to quantitatively analyze industrial acotiamide hydrochloride trihydrate based on its amphoteric structure. The separation is accomplished in a C18 (4.6 mm×150 mm, 5 μm) column with methanol and 20 mmol/L ammonium acetate aqueous solution (pH 6.8) (45:55, V/V) as the mobile phase. The detection wavelength is set at UV 280 nm. The retention time of acotiamide hydrochloride trihydrate is 8 min with good peak shape, and the total analysis time is 23 min. Under the optimized chromatographic condition, the calibration curve of acotiamide hydrochloride trihydrate is linear in the range of 0.0006 and 1.0 mg/mL, detection limit is 0.0002 mg/mL, and both the intra-and inter-day relative standard deviations (RSDs) are lower than 1%. This method contains no ion paring agent, benefiting the rapid balance of chromatographic condition and improving the analysis repeatability and maneuverability. The method can be performed for rapid detection of acotiamide hydrochloride trihydrate and used in the quality control of raw medicine.http://www.ykcs.ac.cn/cn/article/id/742ab818-c3d6-43ac-a7bd-8ee3244cc72bhigh performance liquid chromatography (hplc)acotiamide hydrochloride trihydratesodium octanesulfonatemethanol-ammonium acetatequality control |
| spellingShingle | ZHOU Wei-qun QIAO Jun-qin LIAN Hong-zhen Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography Yankuang ceshi high performance liquid chromatography (hplc) acotiamide hydrochloride trihydrate sodium octanesulfonate methanol-ammonium acetate quality control |
| title | Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography |
| title_full | Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography |
| title_fullStr | Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography |
| title_full_unstemmed | Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography |
| title_short | Determination of Industrial Acotiamide Hydrochloride Trihydrate by High Performance Liquid Chromatography |
| title_sort | determination of industrial acotiamide hydrochloride trihydrate by high performance liquid chromatography |
| topic | high performance liquid chromatography (hplc) acotiamide hydrochloride trihydrate sodium octanesulfonate methanol-ammonium acetate quality control |
| url | http://www.ykcs.ac.cn/cn/article/id/742ab818-c3d6-43ac-a7bd-8ee3244cc72b |
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