Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study
Abstract Background Cystic fibrosis is a debilitating, autosomal recessive disease which results in chronic upper and lower airway infection and inflammation. In this study, four adult patients presenting with cystic fibrosis and chronic rhinosinusitis were recruited. Culture and molecular technique...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | Respiratory Research |
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| Online Access: | https://doi.org/10.1186/s12931-021-01697-w |
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| author | Brett Wagner Mackenzie Camila Dassi Anitha Vivekanandan Melissa Zoing Richard G. Douglas Kristi Biswas |
| author_facet | Brett Wagner Mackenzie Camila Dassi Anitha Vivekanandan Melissa Zoing Richard G. Douglas Kristi Biswas |
| author_sort | Brett Wagner Mackenzie |
| collection | DOAJ |
| description | Abstract Background Cystic fibrosis is a debilitating, autosomal recessive disease which results in chronic upper and lower airway infection and inflammation. In this study, four adult patients presenting with cystic fibrosis and chronic rhinosinusitis were recruited. Culture and molecular techniques were employed to evaluate changes in microbial profiles, host gene expression and antimicrobial resistance (AMR) in the upper respiratory tract over time. Methods Swab samples from the sinonasal cavity were collected at the time of surgery and at follow-up clinics at regular time intervals for up to 18 months. Nucleic acids were extracted, and DNA amplicon sequencing was applied to describe bacterial and fungal composition. In parallel, RNA was used to evaluate the expression of 17 AMR genes and two inflammatory markers (interleukins 6 and 8) using custom qPCR array cards. Molecular results were compared with routine sinus and sputum culture reports within each patient. Results Bacterial amplicon sequencing and swab culture reports from the sinonasal cavity were mostly congruent and relatively stable for each patient across time. The predominant species detected in patients P02 and P04 were Pseudomonas aeruginosa, Staphylococcus aureus in patient P03, and a mixture of Enterobacter and S. aureus in patient P01. Fungal profiles were variable and less subject specific than bacterial communities. Increased expressions of interleukins 6 and 8 were observed in all patients throughout the sampling period compared with other measured genes. The most prevalent AMR gene detected was ampC. However, the prevalence of AMR gene expression was low in all patient samples across varying time-points. Conclusions We observed a surprising degree of stability of sinonasal microbial composition, and inflammatory and AMR gene expression across all patients post sinus surgery. |
| first_indexed | 2024-12-16T15:28:04Z |
| format | Article |
| id | doaj.art-34c4952fd0a547d6bfecde19ff94ad0e |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-12-16T15:28:04Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-34c4952fd0a547d6bfecde19ff94ad0e2022-12-21T22:26:26ZengBMCRespiratory Research1465-993X2021-04-0122111210.1186/s12931-021-01697-wLongitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot studyBrett Wagner Mackenzie0Camila Dassi1Anitha Vivekanandan2Melissa Zoing3Richard G. Douglas4Kristi Biswas5Department of Surgery, The University of AucklandDepartment of Surgery, The University of AucklandDepartment of Surgery, The University of AucklandDepartment of Surgery, The University of AucklandDepartment of Surgery, The University of AucklandDepartment of Surgery, The University of AucklandAbstract Background Cystic fibrosis is a debilitating, autosomal recessive disease which results in chronic upper and lower airway infection and inflammation. In this study, four adult patients presenting with cystic fibrosis and chronic rhinosinusitis were recruited. Culture and molecular techniques were employed to evaluate changes in microbial profiles, host gene expression and antimicrobial resistance (AMR) in the upper respiratory tract over time. Methods Swab samples from the sinonasal cavity were collected at the time of surgery and at follow-up clinics at regular time intervals for up to 18 months. Nucleic acids were extracted, and DNA amplicon sequencing was applied to describe bacterial and fungal composition. In parallel, RNA was used to evaluate the expression of 17 AMR genes and two inflammatory markers (interleukins 6 and 8) using custom qPCR array cards. Molecular results were compared with routine sinus and sputum culture reports within each patient. Results Bacterial amplicon sequencing and swab culture reports from the sinonasal cavity were mostly congruent and relatively stable for each patient across time. The predominant species detected in patients P02 and P04 were Pseudomonas aeruginosa, Staphylococcus aureus in patient P03, and a mixture of Enterobacter and S. aureus in patient P01. Fungal profiles were variable and less subject specific than bacterial communities. Increased expressions of interleukins 6 and 8 were observed in all patients throughout the sampling period compared with other measured genes. The most prevalent AMR gene detected was ampC. However, the prevalence of AMR gene expression was low in all patient samples across varying time-points. Conclusions We observed a surprising degree of stability of sinonasal microbial composition, and inflammatory and AMR gene expression across all patients post sinus surgery.https://doi.org/10.1186/s12931-021-01697-wCystic fibrosisChronic rhinosinusitisAntimicrobial resistanceSinonasal microbiotaCultivation |
| spellingShingle | Brett Wagner Mackenzie Camila Dassi Anitha Vivekanandan Melissa Zoing Richard G. Douglas Kristi Biswas Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study Respiratory Research Cystic fibrosis Chronic rhinosinusitis Antimicrobial resistance Sinonasal microbiota Cultivation |
| title | Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study |
| title_full | Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study |
| title_fullStr | Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study |
| title_full_unstemmed | Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study |
| title_short | Longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis: a pilot study |
| title_sort | longitudinal analysis of sinus microbiota post endoscopic surgery in patients with cystic fibrosis and chronic rhinosinusitis a pilot study |
| topic | Cystic fibrosis Chronic rhinosinusitis Antimicrobial resistance Sinonasal microbiota Cultivation |
| url | https://doi.org/10.1186/s12931-021-01697-w |
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