Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy
MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact de...
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MDPI AG
2023-01-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/277 |
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| author | Yuehui Zhang Maki Hosoki Masamitsu Oshima Toyoko Tajima Mayu Miyagi Swarnalakshmi Raman Resmi Raju Yoshizo Matsuka |
| author_facet | Yuehui Zhang Maki Hosoki Masamitsu Oshima Toyoko Tajima Mayu Miyagi Swarnalakshmi Raman Resmi Raju Yoshizo Matsuka |
| author_sort | Yuehui Zhang |
| collection | DOAJ |
| description | MicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (<i>n</i> = 3) were enrolled along with additional female participants with no medical history of metal allergy (<i>n</i> = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies. |
| first_indexed | 2024-03-11T09:07:32Z |
| format | Article |
| id | doaj.art-34c60a05edfe4c89980c2571b36fbe88 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:07:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-34c60a05edfe4c89980c2571b36fbe882023-11-16T19:15:49ZengMDPI AGBiomedicines2227-90592023-01-0111227710.3390/biomedicines11020277Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal AllergyYuehui Zhang0Maki Hosoki1Masamitsu Oshima2Toyoko Tajima3Mayu Miyagi4Swarnalakshmi Raman5Resmi Raju6Yoshizo Matsuka7Department of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanDepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanDepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanDepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanDepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanDepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanEunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD 20892, USADepartment of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima 7708504, JapanMicroRNA (miRNA) is a short (19–24 nucleotide) endogenous non-protein RNA that exists in the body and controls the translation process from genes to proteins. It has become useful as a diagnostic tool and a potential treatment target in cancer research. To explore the function of miRNA in contact dermatitis, female participants with a positive metal allergy diagnosis (<i>n</i> = 3) were enrolled along with additional female participants with no medical history of metal allergy (<i>n</i> = 3). A patch test was performed on each participant. Peripheral blood was collected from all the participants before the patch test and at days 3 and 7 after starting the patch test. After total RNA was obtained from peripheral blood leukocytes and cDNA was generated, microarray analysis was performed to analyze the large-scale circulating miRNA profile. Real-time polymerase chain reaction (RT-PCR) was then used to clarify the overall target miRNA expression. Downregulation of hsa-let-7d-5p, hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-26b-5p, and hsa-miR-150-5p was found on day 7. Certain miRNAs were confirmed using RT-PCR. These peripheral blood miRNAs could be diagnostic biomarkers for metal allergies.https://www.mdpi.com/2227-9059/11/2/277allergic contact dermatitisbiomarkermetal allergymicroRNAperipheral blood |
| spellingShingle | Yuehui Zhang Maki Hosoki Masamitsu Oshima Toyoko Tajima Mayu Miyagi Swarnalakshmi Raman Resmi Raju Yoshizo Matsuka Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy Biomedicines allergic contact dermatitis biomarker metal allergy microRNA peripheral blood |
| title | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
| title_full | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
| title_fullStr | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
| title_full_unstemmed | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
| title_short | Identification of microRNA Signatures in Peripheral Blood of Young Women as Potential Biomarkers for Metal Allergy |
| title_sort | identification of microrna signatures in peripheral blood of young women as potential biomarkers for metal allergy |
| topic | allergic contact dermatitis biomarker metal allergy microRNA peripheral blood |
| url | https://www.mdpi.com/2227-9059/11/2/277 |
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