In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts
Although <i>Erythrina senegalensis</i> is a plant widely used in traditional medicine in sub-Saharan Africa, its biological properties have been poorly investigated to date. We first characterized by conventional reactions the composition of several stem bark extracts and evaluated in ac...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/8/2583 |
| _version_ | 1827619568060727296 |
|---|---|
| author | Souleymane Fofana Cédric Delporte Rafaèle Calvo Esposito Moussa Ouédraogo Pierre Van Antwerpen Innocent Pierre Guissou Rasmané Semdé Véronique Mathieu |
| author_facet | Souleymane Fofana Cédric Delporte Rafaèle Calvo Esposito Moussa Ouédraogo Pierre Van Antwerpen Innocent Pierre Guissou Rasmané Semdé Véronique Mathieu |
| author_sort | Souleymane Fofana |
| collection | DOAJ |
| description | Although <i>Erythrina senegalensis</i> is a plant widely used in traditional medicine in sub-Saharan Africa, its biological properties have been poorly investigated to date. We first characterized by conventional reactions the composition of several stem bark extracts and evaluated in acellular and cellular assays their pro- or antioxidant properties supported by their high phenolic and flavonoid content, particularly with the methanolic extract. The pro- or antioxidant effects observed did not correlate with their IC<sub>50</sub> concentrations against five cancer cell lines determined by MTT assay. Indeed, the CH<sub>2</sub>Cl<sub>2</sub> extract and its ethyl acetate (EtOAc) subfraction appeared more potent although they harbored lower pro- or antioxidant effects. Nevertheless, at equipotent concentration, both extracts induced ER- and mitochondria-derived vacuoles observed by fluorescent microscopy that further led to non-apoptotic cell death. LC coupled to high resolution MS investigations have been performed to identify chemical compounds of the extracts. These investigations highlighted the presence of compounds formerly isolated from <i>E. senegalensis</i> including senegalensein that could be retrieved only in the EtOAc subfraction but also thirteen other compounds, such as 16:3-Glc-stigmasterol and hexadecanoic acid, whose anticancer properties have been previously reported. Nineteen other compounds remain to be identified. In conclusion, <i>E. senegalensis</i> appeared rich in compounds with antioxidant and anticancer properties, supporting its use in traditional practice and its status as a species of interest for further investigations in anticancer drug research. |
| first_indexed | 2024-03-09T10:30:58Z |
| format | Article |
| id | doaj.art-34c96a08b17846118230589bd927e33b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:30:58Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-34c96a08b17846118230589bd927e33b2023-12-01T21:16:27ZengMDPI AGMolecules1420-30492022-04-01278258310.3390/molecules27082583In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> ExtractsSouleymane Fofana0Cédric Delporte1Rafaèle Calvo Esposito2Moussa Ouédraogo3Pierre Van Antwerpen4Innocent Pierre Guissou5Rasmané Semdé6Véronique Mathieu7Laboratory of Drug Sciences, Higher Institute of Health Sciences (INSSA), Nazi BONI University, Bobo-Dioulasso 01 P.O. Box 1091, Burkina FasoRD3—Pharmacognosy, Bioanalysis and Drug Discovery Unit and Analytical Platform, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), 1050 Brussels, BelgiumProtein Chemistry Unit, Department of General Chemistry I, Faculty of Medicine, Université Libre de Bruxelles, Campus Erasme (CP 609), Route de Lennik, 1070 Brussels, BelgiumLaboratory of Drug Development (LADME), Center of Training, Research and Expertises of Pharmaceutical Sciences (CEA-CFOREM), Training and Research Unit, Health Sciences, Joseph KI-ZERBO University, Ouagadougou 03 P.O. Box 7021, Burkina FasoRD3—Pharmacognosy, Bioanalysis and Drug Discovery Unit and Analytical Platform, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), 1050 Brussels, BelgiumFaculty of Health Sciences, Saint Thomas d’Aquin University, Ouagadougou 06 P.O. Box 10212, Burkina FasoLaboratory of Drug Development (LADME), Center of Training, Research and Expertises of Pharmaceutical Sciences (CEA-CFOREM), Training and Research Unit, Health Sciences, Joseph KI-ZERBO University, Ouagadougou 03 P.O. Box 7021, Burkina FasoDepartment of Pharmacotherapy and Pharmaceuticals, Faculty of Pharmacy, Université Libre de Bruxelles (ULB), 1050 Brussels, BelgiumAlthough <i>Erythrina senegalensis</i> is a plant widely used in traditional medicine in sub-Saharan Africa, its biological properties have been poorly investigated to date. We first characterized by conventional reactions the composition of several stem bark extracts and evaluated in acellular and cellular assays their pro- or antioxidant properties supported by their high phenolic and flavonoid content, particularly with the methanolic extract. The pro- or antioxidant effects observed did not correlate with their IC<sub>50</sub> concentrations against five cancer cell lines determined by MTT assay. Indeed, the CH<sub>2</sub>Cl<sub>2</sub> extract and its ethyl acetate (EtOAc) subfraction appeared more potent although they harbored lower pro- or antioxidant effects. Nevertheless, at equipotent concentration, both extracts induced ER- and mitochondria-derived vacuoles observed by fluorescent microscopy that further led to non-apoptotic cell death. LC coupled to high resolution MS investigations have been performed to identify chemical compounds of the extracts. These investigations highlighted the presence of compounds formerly isolated from <i>E. senegalensis</i> including senegalensein that could be retrieved only in the EtOAc subfraction but also thirteen other compounds, such as 16:3-Glc-stigmasterol and hexadecanoic acid, whose anticancer properties have been previously reported. Nineteen other compounds remain to be identified. In conclusion, <i>E. senegalensis</i> appeared rich in compounds with antioxidant and anticancer properties, supporting its use in traditional practice and its status as a species of interest for further investigations in anticancer drug research.https://www.mdpi.com/1420-3049/27/8/2583<i>E. senegalensis</i>cytotoxicityparaptosisROSvacuolesanticancer |
| spellingShingle | Souleymane Fofana Cédric Delporte Rafaèle Calvo Esposito Moussa Ouédraogo Pierre Van Antwerpen Innocent Pierre Guissou Rasmané Semdé Véronique Mathieu In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts Molecules <i>E. senegalensis</i> cytotoxicity paraptosis ROS vacuoles anticancer |
| title | In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts |
| title_full | In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts |
| title_fullStr | In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts |
| title_full_unstemmed | In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts |
| title_short | In Vitro Antioxidant and Anticancer Properties of Various <i>E. senegalensis</i> Extracts |
| title_sort | in vitro antioxidant and anticancer properties of various i e senegalensis i extracts |
| topic | <i>E. senegalensis</i> cytotoxicity paraptosis ROS vacuoles anticancer |
| url | https://www.mdpi.com/1420-3049/27/8/2583 |
| work_keys_str_mv | AT souleymanefofana invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT cedricdelporte invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT rafaelecalvoesposito invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT moussaouedraogo invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT pierrevanantwerpen invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT innocentpierreguissou invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT rasmanesemde invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts AT veroniquemathieu invitroantioxidantandanticancerpropertiesofvariousiesenegalensisiextracts |