BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage
High oleic acid peanut (HOAP) is extensively embraced in China because of its high nutritional value and enhanced oxidative stability. However, its dissemination has been severely constrained in high altitude and high latitude areas due to chilling stress during sowing, resulting in significant yiel...
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MDPI AG
2022-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/1/18 |
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| author | Hongxi Sun Liang Ren Feiyan Qi Haixin Wang Shutao Yu Ziqi Sun Bingyan Huang Suoyi Han Puxiang Shi Yibo Wang Xinyou Zhang Guoqing Yu |
| author_facet | Hongxi Sun Liang Ren Feiyan Qi Haixin Wang Shutao Yu Ziqi Sun Bingyan Huang Suoyi Han Puxiang Shi Yibo Wang Xinyou Zhang Guoqing Yu |
| author_sort | Hongxi Sun |
| collection | DOAJ |
| description | High oleic acid peanut (HOAP) is extensively embraced in China because of its high nutritional value and enhanced oxidative stability. However, its dissemination has been severely constrained in high altitude and high latitude areas due to chilling stress during sowing, resulting in significant yield loss in these regions. Despite the lack of understanding of the molecular mechanisms underlying low temperature germination (LTG) in HOAP, discovering the quantitative trait loci (QTL) that confer this trait will undoubtedly benefit breeding efforts. In the present study, we identified putative genomic regions and single nucleotide polymorphisms (SNPs) that govern LTG tolerance of HOAP in an F<sub>2</sub> population derived from the cross of chilling-tolerant YH65 and chilling-sensitive FL14 using bulk segregant analysis (BSA). Analysis of ΔSNP-index and Euclidean distance (ED) value association pinpointed the overlapped region to a 2.29 Mb interval on chromosome A05. The candidate interval showed that 122 genes were significantly related to response to abiotic stress and plant–pathogen interaction. Furthermore, an SNP site associated with LTG tolerance was discovered. The SNP site was employed as a Kompetitive Allele Specific PCR (KASP) marker and validated in a universal peanut panel. These findings may provide valuable insight into the molecular mechanism underpinning LTG tolerance and facilitate marker-assisted selective breeding in HOAP. |
| first_indexed | 2024-03-09T13:52:50Z |
| format | Article |
| id | doaj.art-34cc238d11fd47ebb8f709e5745a2851 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T13:52:50Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-34cc238d11fd47ebb8f709e5745a28512023-11-30T20:47:47ZengMDPI AGAgronomy2073-43952022-12-011311810.3390/agronomy13010018BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination StageHongxi Sun0Liang Ren1Feiyan Qi2Haixin Wang3Shutao Yu4Ziqi Sun5Bingyan Huang6Suoyi Han7Puxiang Shi8Yibo Wang9Xinyou Zhang10Guoqing Yu11College of Agronomy, Shenyang Agricultural University, Shenyang 110161, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaHenan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaHenan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaHenan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaHenan Academy of Crop Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou 450002, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110161, ChinaInstitute of Sandy Land Management and Utilization of Liaoning, Fuxin 123000, ChinaHigh oleic acid peanut (HOAP) is extensively embraced in China because of its high nutritional value and enhanced oxidative stability. However, its dissemination has been severely constrained in high altitude and high latitude areas due to chilling stress during sowing, resulting in significant yield loss in these regions. Despite the lack of understanding of the molecular mechanisms underlying low temperature germination (LTG) in HOAP, discovering the quantitative trait loci (QTL) that confer this trait will undoubtedly benefit breeding efforts. In the present study, we identified putative genomic regions and single nucleotide polymorphisms (SNPs) that govern LTG tolerance of HOAP in an F<sub>2</sub> population derived from the cross of chilling-tolerant YH65 and chilling-sensitive FL14 using bulk segregant analysis (BSA). Analysis of ΔSNP-index and Euclidean distance (ED) value association pinpointed the overlapped region to a 2.29 Mb interval on chromosome A05. The candidate interval showed that 122 genes were significantly related to response to abiotic stress and plant–pathogen interaction. Furthermore, an SNP site associated with LTG tolerance was discovered. The SNP site was employed as a Kompetitive Allele Specific PCR (KASP) marker and validated in a universal peanut panel. These findings may provide valuable insight into the molecular mechanism underpinning LTG tolerance and facilitate marker-assisted selective breeding in HOAP.https://www.mdpi.com/2073-4395/13/1/18peanuthigh oleic acidcold toleranceBSA-seqgermination stage |
| spellingShingle | Hongxi Sun Liang Ren Feiyan Qi Haixin Wang Shutao Yu Ziqi Sun Bingyan Huang Suoyi Han Puxiang Shi Yibo Wang Xinyou Zhang Guoqing Yu BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage Agronomy peanut high oleic acid cold tolerance BSA-seq germination stage |
| title | BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage |
| title_full | BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage |
| title_fullStr | BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage |
| title_full_unstemmed | BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage |
| title_short | BSA-Seq Approach Identified Candidate Region and Diagnostic Marker for Chilling Tolerance of High Oleic Acid Peanut at Germination Stage |
| title_sort | bsa seq approach identified candidate region and diagnostic marker for chilling tolerance of high oleic acid peanut at germination stage |
| topic | peanut high oleic acid cold tolerance BSA-seq germination stage |
| url | https://www.mdpi.com/2073-4395/13/1/18 |
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