Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea
Background and purpose: No reports are found on biological activities of Nonea lutea (boraginaceae). The aim of this study was to investigate the antioxidant and antibacterial activities of this plant. Materials and methods: The aerial parts were extracted by three different methods, i.e. meceration...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2018-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-9755-en.html |
| _version_ | 1797943767297163264 |
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| author | Babak Babakhani Fatemeh Janbaz Mohammad Ali Ebrahimzadeh |
| author_facet | Babak Babakhani Fatemeh Janbaz Mohammad Ali Ebrahimzadeh |
| author_sort | Babak Babakhani |
| collection | DOAJ |
| description | Background and purpose: No reports are found on biological activities of Nonea lutea (boraginaceae). The aim of this study was to investigate the antioxidant and antibacterial activities of this plant.
Materials and methods: The aerial parts were extracted by three different methods, i.e. meceration (MA), ultrasonic assisted (US), and soxhlet assisted (SO) extractions with methanol. Antioxidative capacity was assessed by utilizing four methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also determined. Antibacterial activities were evaluated by disc diffusion method against four bacteria.
Results: The highest amount of total phenolic and flavonoid contents were found in SO and US extracts, respectively. In DPPH radical scavenging activity, SO extract (IC50 = 99.14 ± 0.3 µg ml-1) had a higher activity which was significantly different from that of other extracts (P< 0.01). In reducing power, the extracts showed similar activities (P>0.05). The extracts showed weak iron chelating and nitric oxide radical scavenging activities indicating no significant differences (P> 0.05). Findings showed that all extracts had the highest activity against B. subtilis and the least activity against S. aureus. SO extract showed the highest activity against B. subtilis, US extract showed the highest activity against E coli and P. aeruginosa, and MA extract showed the highest activity against S. aureus.
Conclusion: The results indicated that extraction methods significantly affect antioxidant and antibacterial capacities and total phenolic and flavonoid contents. For this plant, SO extraction method was found to be more efficient. |
| first_indexed | 2024-04-10T20:28:22Z |
| format | Article |
| id | doaj.art-34cd189b7f8d487bbdfe0e8e55ead56c |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:28:22Z |
| publishDate | 2018-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-34cd189b7f8d487bbdfe0e8e55ead56c2023-01-25T07:42:30ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792018-01-0127156129145Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea luteaBabak Babakhani0Fatemeh Janbaz1Mohammad Ali Ebrahimzadeh2 Background and purpose: No reports are found on biological activities of Nonea lutea (boraginaceae). The aim of this study was to investigate the antioxidant and antibacterial activities of this plant. Materials and methods: The aerial parts were extracted by three different methods, i.e. meceration (MA), ultrasonic assisted (US), and soxhlet assisted (SO) extractions with methanol. Antioxidative capacity was assessed by utilizing four methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also determined. Antibacterial activities were evaluated by disc diffusion method against four bacteria. Results: The highest amount of total phenolic and flavonoid contents were found in SO and US extracts, respectively. In DPPH radical scavenging activity, SO extract (IC50 = 99.14 ± 0.3 µg ml-1) had a higher activity which was significantly different from that of other extracts (P< 0.01). In reducing power, the extracts showed similar activities (P>0.05). The extracts showed weak iron chelating and nitric oxide radical scavenging activities indicating no significant differences (P> 0.05). Findings showed that all extracts had the highest activity against B. subtilis and the least activity against S. aureus. SO extract showed the highest activity against B. subtilis, US extract showed the highest activity against E coli and P. aeruginosa, and MA extract showed the highest activity against S. aureus. Conclusion: The results indicated that extraction methods significantly affect antioxidant and antibacterial capacities and total phenolic and flavonoid contents. For this plant, SO extraction method was found to be more efficient.http://jmums.mazums.ac.ir/article-1-9755-en.htmlnonnea luteaboraginaceaeantibacterialdisc diffusion methodextraction methodphenolflavonoid |
| spellingShingle | Babak Babakhani Fatemeh Janbaz Mohammad Ali Ebrahimzadeh Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea Journal of Mazandaran University of Medical Sciences nonnea lutea boraginaceae antibacterial disc diffusion method extraction method phenol flavonoid |
| title | Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea |
| title_full | Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea |
| title_fullStr | Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea |
| title_full_unstemmed | Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea |
| title_short | Influence of Different Extraction Methods on Antioxidant and Antibacterial Activities of Nonnea lutea |
| title_sort | influence of different extraction methods on antioxidant and antibacterial activities of nonnea lutea |
| topic | nonnea lutea boraginaceae antibacterial disc diffusion method extraction method phenol flavonoid |
| url | http://jmums.mazums.ac.ir/article-1-9755-en.html |
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