Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage
The efficacy of aerial parts’ organic extracts of diploid and mixoploid Trigonella foenum-graecum L. plants, harvested at three developmental stages (vegetative, flowering and fruiting) was evaluated for their antifungal activity against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and F. ox...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535214000677 |
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| author | Faten Omezzine Mohamed Bouaziz Mejda Daami-Remadi Monique S.J. Simmonds Rabiaa Haouala |
| author_facet | Faten Omezzine Mohamed Bouaziz Mejda Daami-Remadi Monique S.J. Simmonds Rabiaa Haouala |
| author_sort | Faten Omezzine |
| collection | DOAJ |
| description | The efficacy of aerial parts’ organic extracts of diploid and mixoploid Trigonella foenum-graecum L. plants, harvested at three developmental stages (vegetative, flowering and fruiting) was evaluated for their antifungal activity against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and F. oxysporum f. sp. lycopersici (FOL). All tested extracts inhibited FORL and FOL mycelial growth. The organic extracts of diploid plants were found to be less toxic than mixoploid ones and this toxicity varied with the plant developmental stages. The diploids were most toxic, for the two strains, at the fruiting stage; however, mixoploids were more toxic at the vegetative stage for FOL and at flowering one for FORL. FOL was found to be more sensitive to fenugreek extracts when compared to FORL. LC–MS/MS analysis of methanolic extract of fenugreek aerial parts showed eleven different flavonol glycosides (quercetin, kaempferol and vitexin). Five novel components were identified, for the first time in fenugreek aerial parts, as kaempferol 3-O-β-d-glucopyranoside, kaempferol 7-O-glucoside, kaempferol 3-O-α-l-rhamnosyl (1→2) β-d-xyloside, kaempferol 7-O-β-d-glucopyranosyl (1–4) β-d-glucopyranoside and kaempferol 3-O-β-glucosyl (1→2) (6′-O-acetyl)-β-d-galactoside, along with other known compounds of this species. To operate with the maximum efficiency, the allelopathic potential of a given plant, our study showed that it would be advisable to identify the most productive developmental stage of allelochemicals. Similarly, it seems that mixoploidy would be a simple and effective biotechnology tool to improve (in quantity and quality) the allelochemicals’ production, since the extracts’ toxicity of diploid and mixoploid plants, was different. |
| first_indexed | 2024-04-12T13:26:56Z |
| format | Article |
| id | doaj.art-34d0d184593843c192b74c69b13cf5e8 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-12T13:26:56Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-34d0d184593843c192b74c69b13cf5e82022-12-22T03:31:17ZengElsevierArabian Journal of Chemistry1878-53522017-05-0110S2S3622S363110.1016/j.arabjc.2014.03.013Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stageFaten Omezzine0Mohamed Bouaziz1Mejda Daami-Remadi2Monique S.J. Simmonds3Rabiaa Haouala4Department of Biology, Faculty of Sciences of Bizerte, University of Carthage, Amilcar 1054, TunisiaElectro-chemical Environmental Laboratory, ENIS, University of Sfax, Route of Soukra, 3038, TunisiaIntegrated Horticultural Production in the Tunisian Centre East, The Regional Center of Research on Horticulture and Organic Agriculture of Chott-Mariem, Sousse University, Chott-Mariem 4042, TunisiaRoyal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UKDepartment of Biological Sciences and Plant Protection, Agronomic Higher Institute of Chott-Mariem, University of Sousse, Chott-Mariem 4042, Sousse, TunisiaThe efficacy of aerial parts’ organic extracts of diploid and mixoploid Trigonella foenum-graecum L. plants, harvested at three developmental stages (vegetative, flowering and fruiting) was evaluated for their antifungal activity against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) and F. oxysporum f. sp. lycopersici (FOL). All tested extracts inhibited FORL and FOL mycelial growth. The organic extracts of diploid plants were found to be less toxic than mixoploid ones and this toxicity varied with the plant developmental stages. The diploids were most toxic, for the two strains, at the fruiting stage; however, mixoploids were more toxic at the vegetative stage for FOL and at flowering one for FORL. FOL was found to be more sensitive to fenugreek extracts when compared to FORL. LC–MS/MS analysis of methanolic extract of fenugreek aerial parts showed eleven different flavonol glycosides (quercetin, kaempferol and vitexin). Five novel components were identified, for the first time in fenugreek aerial parts, as kaempferol 3-O-β-d-glucopyranoside, kaempferol 7-O-glucoside, kaempferol 3-O-α-l-rhamnosyl (1→2) β-d-xyloside, kaempferol 7-O-β-d-glucopyranosyl (1–4) β-d-glucopyranoside and kaempferol 3-O-β-glucosyl (1→2) (6′-O-acetyl)-β-d-galactoside, along with other known compounds of this species. To operate with the maximum efficiency, the allelopathic potential of a given plant, our study showed that it would be advisable to identify the most productive developmental stage of allelochemicals. Similarly, it seems that mixoploidy would be a simple and effective biotechnology tool to improve (in quantity and quality) the allelochemicals’ production, since the extracts’ toxicity of diploid and mixoploid plants, was different.http://www.sciencedirect.com/science/article/pii/S1878535214000677Antifungal activityDevelopmental stageFlavonol glycosidesPloidy level |
| spellingShingle | Faten Omezzine Mohamed Bouaziz Mejda Daami-Remadi Monique S.J. Simmonds Rabiaa Haouala Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage Arabian Journal of Chemistry Antifungal activity Developmental stage Flavonol glycosides Ploidy level |
| title | Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage |
| title_full | Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage |
| title_fullStr | Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage |
| title_full_unstemmed | Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage |
| title_short | Chemical composition and antifungal activity of Trigonella foenum-graecum L. varied with plant ploidy level and developmental stage |
| title_sort | chemical composition and antifungal activity of trigonella foenum graecum l varied with plant ploidy level and developmental stage |
| topic | Antifungal activity Developmental stage Flavonol glycosides Ploidy level |
| url | http://www.sciencedirect.com/science/article/pii/S1878535214000677 |
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