Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
Abstract Background Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tr...
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04500-8 |
| _version_ | 1827634937642090496 |
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| author | Guo Liu Zhihua Wu Jianzhong Luo Chubiao Wang Xiuhua Shang Guowu Zhang |
| author_facet | Guo Liu Zhihua Wu Jianzhong Luo Chubiao Wang Xiuhua Shang Guowu Zhang |
| author_sort | Guo Liu |
| collection | DOAJ |
| description | Abstract Background Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes. Therefore, molecular level research on the vascular cambium of different tree ages can lead to the identification of both key and related genes involved in wood formation and further explain the molecular regulation mechanism of wood formation. Results In the present study, RNA-Seq and Pac-Bio Iso-Seq were used for profiling gene expression changes in Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) vascular cambium at four different ages. A total of 59,770 non-redundant transcripts and 1892 differentially expressed genes (DEGs) were identified. The expression trends of the DEGs related to cell division and differentiation, cell wall biosynthesis, phytohormone, and transcription factors were analyzed. The DEGs encoding expansin, kinesin, cycline, PAL, GRP9, KNOX, C2C2-dof, REV, etc., were highly expressed in E. urograndis at three years old, leading to positive effects on growth and development. Moreover, some gene family members, such as NAC, MYB, HD-ZIP III, RPK, and RAP, play different regulatory roles in wood formation because of their sophisticated transcriptional network and function redundantly. Conclusions These candidate genes are a potential resource to further study wood formation, especially in fast-growing and adaptable eucalyptus. The results may also serve as a basis for further research to unravel the molecular mechanism underlying wood formation. |
| first_indexed | 2024-03-09T15:21:15Z |
| format | Article |
| id | doaj.art-34d1a6b493094affa8c7e6ab9f705709 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T15:21:15Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-34d1a6b493094affa8c7e6ab9f7057092023-11-26T12:47:16ZengBMCBMC Plant Biology1471-22292023-10-0123111710.1186/s12870-023-04500-8Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different agesGuo Liu0Zhihua Wu1Jianzhong Luo2Chubiao Wang3Xiuhua Shang4Guowu Zhang5State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryResearch Institute of Fast-Growing Trees, Chinese Academy of ForestryState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of ForestryResearch Institute of Fast-Growing Trees, Chinese Academy of ForestryResearch Institute of Fast-Growing Trees, Chinese Academy of ForestryResearch Institute of Fast-Growing Trees, Chinese Academy of ForestryAbstract Background Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes. Therefore, molecular level research on the vascular cambium of different tree ages can lead to the identification of both key and related genes involved in wood formation and further explain the molecular regulation mechanism of wood formation. Results In the present study, RNA-Seq and Pac-Bio Iso-Seq were used for profiling gene expression changes in Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) vascular cambium at four different ages. A total of 59,770 non-redundant transcripts and 1892 differentially expressed genes (DEGs) were identified. The expression trends of the DEGs related to cell division and differentiation, cell wall biosynthesis, phytohormone, and transcription factors were analyzed. The DEGs encoding expansin, kinesin, cycline, PAL, GRP9, KNOX, C2C2-dof, REV, etc., were highly expressed in E. urograndis at three years old, leading to positive effects on growth and development. Moreover, some gene family members, such as NAC, MYB, HD-ZIP III, RPK, and RAP, play different regulatory roles in wood formation because of their sophisticated transcriptional network and function redundantly. Conclusions These candidate genes are a potential resource to further study wood formation, especially in fast-growing and adaptable eucalyptus. The results may also serve as a basis for further research to unravel the molecular mechanism underlying wood formation.https://doi.org/10.1186/s12870-023-04500-8TranscriptomeVascular cambiumEucalyptus urophylla × Eucalyptus grandisDifferent agesTranscription factorPhytohormone |
| spellingShingle | Guo Liu Zhihua Wu Jianzhong Luo Chubiao Wang Xiuhua Shang Guowu Zhang Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages BMC Plant Biology Transcriptome Vascular cambium Eucalyptus urophylla × Eucalyptus grandis Different ages Transcription factor Phytohormone |
| title | Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages |
| title_full | Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages |
| title_fullStr | Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages |
| title_full_unstemmed | Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages |
| title_short | Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages |
| title_sort | genes expression profiles in vascular cambium of eucalyptus urophylla eucalyptus grandis at different ages |
| topic | Transcriptome Vascular cambium Eucalyptus urophylla × Eucalyptus grandis Different ages Transcription factor Phytohormone |
| url | https://doi.org/10.1186/s12870-023-04500-8 |
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