Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock.
Interferon Regulatory Factor (IRF) 6, a member of the IRF family, is essential for epidermal and orofacial embryonic development. Irf6 is strongly expressed in keratinocytes, in which it regulates epidermal proliferation, differentiation, and migration. A recent role for Irf6 in Toll-like receptor 2...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4817988?pdf=render |
| _version_ | 1818863250745327616 |
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| author | Sophie Joly Lindsey Rhea Paige Volk Jessica G Moreland Martine Dunnwald |
| author_facet | Sophie Joly Lindsey Rhea Paige Volk Jessica G Moreland Martine Dunnwald |
| author_sort | Sophie Joly |
| collection | DOAJ |
| description | Interferon Regulatory Factor (IRF) 6, a member of the IRF family, is essential for epidermal and orofacial embryonic development. Irf6 is strongly expressed in keratinocytes, in which it regulates epidermal proliferation, differentiation, and migration. A recent role for Irf6 in Toll-like receptor 2-dependent chemokine gene expression was also reported in an epithelial cell line. However, a function for Irf6 in innate immune cells was not previously reported. In the present study, we investigated the expression and function of Irf6 in bone marrow-derived neutrophils and macrophages. We show here, using a conditional knockout of Irf6 in lysosymeM expressing cells, that Irf6 is required for resistance to LPS-induced endotoxic shock. In addition, Irf6-deficient bone marrow-derived neutrophils exhibited increased chemotactic index and velocity compared with wild-type cells in vitro. TLR4-specific KC and IL6 secretions were upregulated in Irf6-deficient bone marrow-derived macrophages in vitro. These cells also exhibited an increased level of phosphorylated IkBa. Collectively, our findings suggest a role for Irf6 in the resistance to endotoxic shock due to NFk-B-mediated alteration of cytokine production. |
| first_indexed | 2024-12-19T10:12:47Z |
| format | Article |
| id | doaj.art-34d21b2d616a468fa270a247bd9ee0da |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T10:12:47Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-34d21b2d616a468fa270a247bd9ee0da2022-12-21T20:26:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015238510.1371/journal.pone.0152385Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock.Sophie JolyLindsey RheaPaige VolkJessica G MorelandMartine DunnwaldInterferon Regulatory Factor (IRF) 6, a member of the IRF family, is essential for epidermal and orofacial embryonic development. Irf6 is strongly expressed in keratinocytes, in which it regulates epidermal proliferation, differentiation, and migration. A recent role for Irf6 in Toll-like receptor 2-dependent chemokine gene expression was also reported in an epithelial cell line. However, a function for Irf6 in innate immune cells was not previously reported. In the present study, we investigated the expression and function of Irf6 in bone marrow-derived neutrophils and macrophages. We show here, using a conditional knockout of Irf6 in lysosymeM expressing cells, that Irf6 is required for resistance to LPS-induced endotoxic shock. In addition, Irf6-deficient bone marrow-derived neutrophils exhibited increased chemotactic index and velocity compared with wild-type cells in vitro. TLR4-specific KC and IL6 secretions were upregulated in Irf6-deficient bone marrow-derived macrophages in vitro. These cells also exhibited an increased level of phosphorylated IkBa. Collectively, our findings suggest a role for Irf6 in the resistance to endotoxic shock due to NFk-B-mediated alteration of cytokine production.http://europepmc.org/articles/PMC4817988?pdf=render |
| spellingShingle | Sophie Joly Lindsey Rhea Paige Volk Jessica G Moreland Martine Dunnwald Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. PLoS ONE |
| title | Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. |
| title_full | Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. |
| title_fullStr | Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. |
| title_full_unstemmed | Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. |
| title_short | Interferon Regulatory Factor 6 Has a Protective Role in the Host Response to Endotoxic Shock. |
| title_sort | interferon regulatory factor 6 has a protective role in the host response to endotoxic shock |
| url | http://europepmc.org/articles/PMC4817988?pdf=render |
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