IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES)
Objective: To study the morphophysiological features of burn wound healing in rats using a polysaccharide gel form based on pectin and polysaccharide from apricot gum with encapsulated sea buckthorn oil. Methods: The study was carried out on two groups of laboratory rats with 18 animals in each g...
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| Format: | Article |
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Avicenna Tajik State Medical University
2023-02-01
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| Series: | Паёми Сино |
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| Online Access: | https://doi.org/10.25005/2074-0581-2023-25-1-84-93 |
| _version_ | 1797796918048325632 |
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| author | M.A. OLIMOV M.U. SHAROFOVA F.M. KHODZHAEVA A.D. KHOLBEKOV J.T. BOBOKALONOV |
| author_facet | M.A. OLIMOV M.U. SHAROFOVA F.M. KHODZHAEVA A.D. KHOLBEKOV J.T. BOBOKALONOV |
| author_sort | M.A. OLIMOV |
| collection | DOAJ |
| description | Objective: To study the morphophysiological features of burn wound healing in rats using a polysaccharide gel form based on pectin and polysaccharide from apricot gum with encapsulated sea buckthorn oil.
Methods: The study was carried out on two groups of laboratory rats with 18 animals in each group. A skin burn injury was inflicted in both groups (for 5 seconds at a temperature of 150°C). In the main group, in order to facilitate regeneration, a new gel form based on a polysaccharide composite with encapsulated sea buckthorn oil was applied to the burn site. In the control group, instead of the gel, a 3% hydrogen peroxide solution was applied to the surface of the wound. The skin, subcutaneous tissue, and muscles were sampled for morphological studies, sample size being 2×2 cm. The tissue was sampled within unchanged skin and processed for histological examination. Histological slides were stained with hematoxylin-eosin and viewed under Axiostar PLUS microscope (Carl Zeiss, Germany).
Results: In the control group, physiological wound healing took place which corresponds to incomplete healing (reparative regeneration) and the formation of scars. In the main group after the gel treatment, the formation of granulation tissue within the epidermis, dermis, and subcutaneous fat was accelerated. It was noted that the use of the proposed gel limited destructive changes, and contributed to the steady maturation of young connective tissue, ensuring the process of re-epithelialization and complete closure of the defect
Conclusion: The proposed polysaccharide gel with encapsulated sea buckthorn oil has great potential for use as a dressing for burn wounds to expedite their healing. |
| first_indexed | 2024-03-13T03:40:21Z |
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| id | doaj.art-34d30d8a74d74bc2ab6e2a3316a98b92 |
| institution | Directory Open Access Journal |
| issn | 2074-0581 2959-6327 |
| language | English |
| last_indexed | 2024-03-13T03:40:21Z |
| publishDate | 2023-02-01 |
| publisher | Avicenna Tajik State Medical University |
| record_format | Article |
| series | Паёми Сино |
| spelling | doaj.art-34d30d8a74d74bc2ab6e2a3316a98b922023-06-23T08:49:19ZengAvicenna Tajik State Medical UniversityПаёми Сино2074-05812959-63272023-02-01251849310.25005/2074-0581-2023-25-1-84-93IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES)M.A. OLIMOV0M.U. SHAROFOVA1F.M. KHODZHAEVA2A.D. KHOLBEKOV3J.T. BOBOKALONOV4Department of Morphology, Faculty of Medicine, Tajik National University, Dushanbe, Republic of Tajikistannstitute of Avicenna's Medicine and Pharmacology, Dushanbe, Republic of Tajikistan. Center for Research of Innovative Technologies of the National Academy of Sciences of Tajikistan, Dushanbe, Republic of TajikistanDepartment of Pharmacology with a Course of Clinical Pharmacology, Avicenna Tajik State Medical University, Dushanbe, Republic of TajikistanDepartment of Morphology, Faculty of Medicine, Tajik National University, Dushanbe, Republic of TajikistanLaboratory of Biopolymers, Institute of Chemistry named after V.I. Nikitin, National Academy of Sciences of Tajikistan, Dushanbe, Republic of TajikistanObjective: To study the morphophysiological features of burn wound healing in rats using a polysaccharide gel form based on pectin and polysaccharide from apricot gum with encapsulated sea buckthorn oil. Methods: The study was carried out on two groups of laboratory rats with 18 animals in each group. A skin burn injury was inflicted in both groups (for 5 seconds at a temperature of 150°C). In the main group, in order to facilitate regeneration, a new gel form based on a polysaccharide composite with encapsulated sea buckthorn oil was applied to the burn site. In the control group, instead of the gel, a 3% hydrogen peroxide solution was applied to the surface of the wound. The skin, subcutaneous tissue, and muscles were sampled for morphological studies, sample size being 2×2 cm. The tissue was sampled within unchanged skin and processed for histological examination. Histological slides were stained with hematoxylin-eosin and viewed under Axiostar PLUS microscope (Carl Zeiss, Germany). Results: In the control group, physiological wound healing took place which corresponds to incomplete healing (reparative regeneration) and the formation of scars. In the main group after the gel treatment, the formation of granulation tissue within the epidermis, dermis, and subcutaneous fat was accelerated. It was noted that the use of the proposed gel limited destructive changes, and contributed to the steady maturation of young connective tissue, ensuring the process of re-epithelialization and complete closure of the defect Conclusion: The proposed polysaccharide gel with encapsulated sea buckthorn oil has great potential for use as a dressing for burn wounds to expedite their healing.https://doi.org/10.25005/2074-0581-2023-25-1-84-93burn woundwound healingpolysaccharide gelpectinapricot gumsea buckthorn oil (hippophae rhamnoides). |
| spellingShingle | M.A. OLIMOV M.U. SHAROFOVA F.M. KHODZHAEVA A.D. KHOLBEKOV J.T. BOBOKALONOV IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) Паёми Сино burn wound wound healing polysaccharide gel pectin apricot gum sea buckthorn oil (hippophae rhamnoides). |
| title | IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) |
| title_full | IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) |
| title_fullStr | IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) |
| title_full_unstemmed | IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) |
| title_short | IN VIVO STUDY OF WOUND-HEALING ACTIVITY OF POLYSACCHARIDE GEL WITH ENCAPSULATED SEA-BUCKTHORN OIL (HIPPOPHAE RHAMNOIDES) |
| title_sort | in vivo study of wound healing activity of polysaccharide gel with encapsulated sea buckthorn oil hippophae rhamnoides |
| topic | burn wound wound healing polysaccharide gel pectin apricot gum sea buckthorn oil (hippophae rhamnoides). |
| url | https://doi.org/10.25005/2074-0581-2023-25-1-84-93 |
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