Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content
In the United States, over three million adults suffer from inflammatory bowel disease (IBD). The gut microbiome, host immune response, and nutrient-microbial interactions are known to play a role in IBD. The relationship between dairy and IBD is controversial; thus, the objectives of this study wer...
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MDPI AG
2022-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/23/5145 |
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| author | Chelsea Garcia Liya Anto Christopher N. Blesso |
| author_facet | Chelsea Garcia Liya Anto Christopher N. Blesso |
| author_sort | Chelsea Garcia |
| collection | DOAJ |
| description | In the United States, over three million adults suffer from inflammatory bowel disease (IBD). The gut microbiome, host immune response, and nutrient-microbial interactions are known to play a role in IBD. The relationship between dairy and IBD is controversial; thus, the objectives of this study were to identify how milk polar lipids (MPLs) and anhydrous milk fat affect colitis disease activity, the colonic transcriptome, and the gut microbiome in a mouse model of chemical-induced colitis. Male and female C57BL/6J mice (n = 120) were randomized into either a low (5% w/w) milk fat or a high (21% w/w) milk fat diet supplemented with either 0%, 1%, or 2% w/w of MPLs for three weeks (n = 10/group/sex). Afterwards, colitis was induced using 1% dextran sodium sulfate in drinking water for five days (colitis induction) and then switched to regular water for five days (colitis recovery). Mice fed added MPLs were protected against colitis when fed a high-fat diet, while added MPLs during low-fat diet attenuated disease activity during the colitis induction period yet promoted colitis and inflammation in male mice during the recovery period. Dietary fat content can alter colitis and influence the anti-inflammatory effect of milk polar lipids. |
| first_indexed | 2024-03-09T17:35:30Z |
| format | Article |
| id | doaj.art-34d8057985664a8d83113d6f53bd3b38 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T17:35:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-34d8057985664a8d83113d6f53bd3b382023-11-24T11:52:55ZengMDPI AGNutrients2072-66432022-12-011423514510.3390/nu14235145Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat ContentChelsea Garcia0Liya Anto1Christopher N. Blesso2Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USADepartment of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USADepartment of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USAIn the United States, over three million adults suffer from inflammatory bowel disease (IBD). The gut microbiome, host immune response, and nutrient-microbial interactions are known to play a role in IBD. The relationship between dairy and IBD is controversial; thus, the objectives of this study were to identify how milk polar lipids (MPLs) and anhydrous milk fat affect colitis disease activity, the colonic transcriptome, and the gut microbiome in a mouse model of chemical-induced colitis. Male and female C57BL/6J mice (n = 120) were randomized into either a low (5% w/w) milk fat or a high (21% w/w) milk fat diet supplemented with either 0%, 1%, or 2% w/w of MPLs for three weeks (n = 10/group/sex). Afterwards, colitis was induced using 1% dextran sodium sulfate in drinking water for five days (colitis induction) and then switched to regular water for five days (colitis recovery). Mice fed added MPLs were protected against colitis when fed a high-fat diet, while added MPLs during low-fat diet attenuated disease activity during the colitis induction period yet promoted colitis and inflammation in male mice during the recovery period. Dietary fat content can alter colitis and influence the anti-inflammatory effect of milk polar lipids.https://www.mdpi.com/2072-6643/14/23/5145colon transcriptomedietary fatmicrobiomeinflammationdairysphingolipids |
| spellingShingle | Chelsea Garcia Liya Anto Christopher N. Blesso Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content Nutrients colon transcriptome dietary fat microbiome inflammation dairy sphingolipids |
| title | Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content |
| title_full | Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content |
| title_fullStr | Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content |
| title_full_unstemmed | Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content |
| title_short | Effects of Milk Polar Lipids on DSS-Induced Colitis Severity Are Dependent on Dietary Fat Content |
| title_sort | effects of milk polar lipids on dss induced colitis severity are dependent on dietary fat content |
| topic | colon transcriptome dietary fat microbiome inflammation dairy sphingolipids |
| url | https://www.mdpi.com/2072-6643/14/23/5145 |
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