New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish
Functional bacteria that could biotransform triterpenoids may exist in the diverse microflora of fish intestines. Ganoderic acid A (GAA) is a major triterpenoid from the medicinal fungus Ganoderma lucidum. In studying the microbial biotransformation of GAA, dozens of intestinal bacteria were isolate...
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MDPI AG
2018-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/9/2345 |
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| author | Te-Sheng Chang Chien-Min Chiang Tzi-Yuan Wang Chun-Hsien Lee Yu-Wen Lee Jiumn-Yih Wu |
| author_facet | Te-Sheng Chang Chien-Min Chiang Tzi-Yuan Wang Chun-Hsien Lee Yu-Wen Lee Jiumn-Yih Wu |
| author_sort | Te-Sheng Chang |
| collection | DOAJ |
| description | Functional bacteria that could biotransform triterpenoids may exist in the diverse microflora of fish intestines. Ganoderic acid A (GAA) is a major triterpenoid from the medicinal fungus Ganoderma lucidum. In studying the microbial biotransformation of GAA, dozens of intestinal bacteria were isolated from the excreta of zebrafish. The bacteria’s ability to catalyze GAA were determined using ultra-performance liquid chromatography analysis. One positive strain, GA A07, was selected for functional studies. GA A07 was confirmed as Bacillus sp., based on the DNA sequences of the 16S rRNA gene. The biotransformed metabolite was purified with the preparative high-performance liquid chromatography method and identified as GAA-15-O-β-glucoside, based on the mass and nuclear magnetic resonance spectral data. The present study is the first to report the glycosylation of Ganoderma triterpenoids. Moreover, 15-O-glycosylation is a new microbial biotransformation of triterpenoids, and the biotransformed metabolite, GAA-15-O-β-glucoside, is a new compound. |
| first_indexed | 2024-12-13T14:46:34Z |
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| id | doaj.art-34d97a72645f4f3db663e8a1f65bb5c9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T14:46:34Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-34d97a72645f4f3db663e8a1f65bb5c92022-12-21T23:41:27ZengMDPI AGMolecules1420-30492018-09-01239234510.3390/molecules23092345molecules23092345New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of ZebrafishTe-Sheng Chang0Chien-Min Chiang1Tzi-Yuan Wang2Chun-Hsien Lee3Yu-Wen Lee4Jiumn-Yih Wu5Department of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, TaiwanDepartment of Biotechnology, Chia Nan University of Pharmacy and Science, No. 60, Sec. 1, Erh-Jen Rd., Jen-Te District, Tainan 71710, TaiwanBiodiversity Research Center, Academia Sinica, Taipei 115, TaiwanDepartment of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, TaiwanDepartment of Biological Sciences and Technology, National University of Tainan, No. 33, Sec. 2, Shu-Lin St., Tainan 70005, TaiwanDepartment of Food Science, National Quemoy University, No. 1, University Rd., Jin-Ning Township, Kinmen County 892, TaiwanFunctional bacteria that could biotransform triterpenoids may exist in the diverse microflora of fish intestines. Ganoderic acid A (GAA) is a major triterpenoid from the medicinal fungus Ganoderma lucidum. In studying the microbial biotransformation of GAA, dozens of intestinal bacteria were isolated from the excreta of zebrafish. The bacteria’s ability to catalyze GAA were determined using ultra-performance liquid chromatography analysis. One positive strain, GA A07, was selected for functional studies. GA A07 was confirmed as Bacillus sp., based on the DNA sequences of the 16S rRNA gene. The biotransformed metabolite was purified with the preparative high-performance liquid chromatography method and identified as GAA-15-O-β-glucoside, based on the mass and nuclear magnetic resonance spectral data. The present study is the first to report the glycosylation of Ganoderma triterpenoids. Moreover, 15-O-glycosylation is a new microbial biotransformation of triterpenoids, and the biotransformed metabolite, GAA-15-O-β-glucoside, is a new compound.http://www.mdpi.com/1420-3049/23/9/2345biotransformationGanoderma lucidumganoderic acid ABacillus |
| spellingShingle | Te-Sheng Chang Chien-Min Chiang Tzi-Yuan Wang Chun-Hsien Lee Yu-Wen Lee Jiumn-Yih Wu New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish Molecules biotransformation Ganoderma lucidum ganoderic acid A Bacillus |
| title | New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish |
| title_full | New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish |
| title_fullStr | New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish |
| title_full_unstemmed | New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish |
| title_short | New Triterpenoid from Novel Triterpenoid 15-O-Glycosylation on Ganoderic Acid A by Intestinal Bacteria of Zebrafish |
| title_sort | new triterpenoid from novel triterpenoid 15 o glycosylation on ganoderic acid a by intestinal bacteria of zebrafish |
| topic | biotransformation Ganoderma lucidum ganoderic acid A Bacillus |
| url | http://www.mdpi.com/1420-3049/23/9/2345 |
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